New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 331270867 (1.110)5 (0.090) 5/266 7.6 8.0% coding (70/945 nt) truB tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase
?NC_000913_3_pae_tpiA = 3312724 52 (0.920)coding (54/945 nt) truB tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTTTGTCGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/3312555‑3312708
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttgctggaTAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTGGCGACCGGCATGTTGCCGATTTGCCTCGGGGAAGCGACGAAGTTTTCCCAG  <  NC_000913_3_pae_tpiA/3312724‑3312577
                                                                                                                                                                                                                                                                                                              
GTTTGTCGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGATACTTGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTG                                                                                                                                                           >  1:940084/1‑149
 TTTGTCGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGC                                                                                                                                                          >  1:364993/1‑149
    GTCGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGG                                                                                                                                                       >  1:436876/1‑149
     TCGGAGCCCAGCACATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGA                                                                                                                                                      <  1:548386/149‑1
      CGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTCGCGCTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGcc                                                                                                                                                     >  2:16157/1‑147
                                 TCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGTCAGCGCGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGATAAACCTCAGGGTATGTCCAGCAACGAT                                                                                                                          <  1:818656/149‑1
                                                                               GTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGATAAACCTCAGGGTATGTCC                                                                                                                                   <  1:523183/94‑1
                                                                               GTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGATAAACCTCAGGGTATGTCC                                                                                                                                   >  2:523183/1‑94
                                                                                                                                          GCGCATTGTTGCTGGATAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGG                                                                              <  1:370157/88‑1
                                                                                                                                          GCGCATTGTTGCTGGATAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGG                                                                              >  2:370157/1‑88
                                                                                                                                                     CTGGATAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTGGCGACCGGCATGTTGCCGATTTGCCTCGGGGAAGCGACGAAGTTTTC      >  2:936755/1‑149
                                                                                                                                                      TGGATAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTGGCGCCCGGCATGTTGCCGATTCGCCTCGGGGAAGCGGCGAAGCTTTCC     >  2:782368/1‑149
                                                                                                                                                      TGGATAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTGGCGACCGGCATGTTGCCGATTTGCCTCGGGGAAGCGACGAAGTTTTCC     >  2:1044768/1‑149
                                                                                                                                                      TGGATAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTG                                                     >  1:923527/1‑101
                                                                                                                                                      TGGATAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTG                                                     <  2:923527/101‑1
                                                                                                                                                      TGGAAAAACCGCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGGGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTGGCGACCGGCATGTTGCCGATTTGCCTCGGGGAAGCGACGAAGTTTTCC     <  2:958087/149‑1
                                                                                                                                                        GATAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTGGCGACCGGCATGTTGCCGCTTTGCCTCGGGGAAGCGACAAAGCTTTCCC  >  2:440247/1‑148
                                                                                                                                                        GATAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTGGCGACCGGCATGTTGCCGATTTGCCTCGGGGAAGCGACGAAGTTTTCCCA   <  2:153552/149‑1
                                                                                                                                                         ATAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTGGCGACCGGCATGTTGCCGATTTGCCTCGGGGAAGCGACGAAGTTTGCCCAG  <  2:916166/149‑1
                                                                                                                                                                                                                                                                                                              
GTTTGTCGGAGTCCAGCAGATACTGGGAAAACTTCGTCGCTTCCCCGAGGCAAATCGGCAACATGCCGGTCGCCAGCGGGTCCAGCGCACCGGTATGCCCGGCACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/3312555‑3312708
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttgctggaTAAACCTCAGGGTATGTCCAGCAACGATGCGCTGCAAAAAGTGAAACGTATATATAACGCCAACCGTGCCGGGCATACCGGTGCGCTGGACCCGCTGGCGACCGGCATGTTGCCGATTTGCCTCGGGGAAGCGACGAAGTTTTCCCAG  <  NC_000913_3_pae_tpiA/3312724‑3312577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.