New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 133852424 (0.850)4 (0.160) 4/256 3.3 14.9% intergenic (+18/+46) acnA/ribA aconitate hydratase 1; aconitase A/GTP cyclohydrolase II
?NC_000913_3_pae_tpiA = 1338535 24 (0.940)intergenic (+29/+35) acnA/ribA aconitate hydratase 1; aconitase A/GTP cyclohydrolase II
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGACACCGCGACGGAGTTGACCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/1338431‑1338524
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atttgcttgccggAAGCAAAATAACCGGCAAGCAAATAGTTGTTACTTCAACATATTACGAATGACATAATGCAAAATGCCGTCGTTCTGGTAGTAGGTCAACTCCGTCGCGGTGTCGATACGACAACGGCAGGGTACGACTTCCTG  <  NC_000913_3_pae_tpiA/1338535‑1338402
                                                                                                                                                                                                                                    
ccACACCGAGACGGAGTTGACTTACTACCATAACGACTGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAATAACTATTTGCTAGACGGAAGGAAAATAACCGGCAAGCAAATAGTTGTTACTTCAACATATTACGAATGACAT                                                                                 <  1:480357/147‑1
                                  GACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGAAGCAAAATAACCGGCAAGCAAATAGTTG                                                                                                           >  1:110658/1‑89
                                  GACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGAAGCAAAATAACCGGCAAGCAAATAGTTG                                                                                                           <  2:110658/89‑1
                                                                               tTATTTGCTTGCTGGAAGCAAAATAACCGGCAAGCAAATAGTTGTTACTTCAACATATTACGAATGACATAATGCAAAATGCCGTCGTTCTGGTAGTAGGTCAACTCCGTCGCGGTGTCGATACGACAACGGCAGGGTACGACTTCCTG  <  2:489800/148‑1
                                                                                                                                                                                                                                    
CGACACCGCGACGGAGTTGACCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/1338431‑1338524
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atttgcttgccggAAGCAAAATAACCGGCAAGCAAATAGTTGTTACTTCAACATATTACGAATGACATAATGCAAAATGCCGTCGTTCTGGTAGTAGGTCAACTCCGTCGCGGTGTCGATACGACAACGGCAGGGTACGACTTCCTG  <  NC_000913_3_pae_tpiA/1338535‑1338402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.