New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	1570533 =	34 (1.200)	4 (0.150)	4/262	3.3	10.9%	intergenic (‑44/+112)	gadC/gadB	glutamate:gamma‑aminobutyric acid antiporter/glutamate decarboxylase B, PLP‑dependent
	?	NC_000913_3_pae_tpiA	1570558 =	34 (1.300)					intergenic (‑69/+87)	gadC/gadB	glutamate:gamma‑aminobutyric acid antiporter/glutamate decarboxylase B, PLP‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATAC  >  NC_000913_3_pae_tpiA/1570385‑1570679                                                                                                                                                     |                                                                                                                                                   gAAGGTCGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTATCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGCATTCCTGTCGGAACCGCACCTGttt                                                                                                                                                    <  1:452177/149‑1 (MQ=255)               cATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCCCCTGTTTTGTTCTGAGGCGTa                                                                                                                                      >  1:421407/1‑149 (MQ=255)               cATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa                                                                                                                                      <  2:206574/149‑1 (MQ=255)                 tAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTAtt                                                                                                                                    <  2:328104/149‑1 (MQ=255)                                          ttATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAAt                                                                                                           >  1:437327/1‑149 (MQ=255)                                                   aGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTGCTGTTGGAACCATATTCTCCACCTAAAACGGTATTCCTGTCGGCACCGCAGCGGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCGGTGaa                                                                                                  >  1:171119/1‑149 (MQ=255)                                                          aaGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACtt                                                                                           >  2:353184/1‑149 (MQ=255)                                                                      ctgctTAGGTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTcc                                                                               <  2:67360/149‑1 (MQ=255)                                                                          ttAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATAtttt                                                                                                                      >  2:193101/1‑106 (MQ=255)                                                                          ttAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATAtttt                                                                                                                      <  1:193101/106‑1 (MQ=255)                                                                          ttAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGaa                                                                           >  2:441485/1‑149 (MQ=255)                                                                             gCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTcc                                                                               >  2:102160/1‑142 (MQ=255)                                                                             gCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTcc                                                                               <  1:102160/142‑1 (MQ=255)                                                                                 tACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGtt                                                                    <  1:277754/149‑1 (MQ=255)                                                                                         tgtAGTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCgg                                                            <  2:437327/149‑1 (MQ=255)                                                                                            aCTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTCTCCCGAAACGGGGTCGTTTCGTGac                                                         >  1:91408/1‑149 (MQ=255)                                                                                                      ccATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACaccgttaccg                                               >  1:337738/1‑149 (MQ=255)                                                                                                       cATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACaccgttaccgt                                              <  2:337738/149‑1 (MQ=255)                                                                                                                  ccTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTc                                                                                >  1:289228/1‑104 (MQ=255)                                                                                                                  ccTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTc                                                                                <  2:289228/104‑1 (MQ=255)                                                                                                                               tCCTGTTGGAACCGCACCGGTTTTGTTCTGACGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAACTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTaaa                      <  2:409779/149‑1 (MQ=255)                                                                                                                                cgtGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACAACGTTACCGTTAAACGTTATCAGGTTTGTTTaacg                     <  2:421407/147‑3 (MQ=255)                                                                                                                                 cTGTCGGGACCGCACCTGTTTTGTGCTGAAGCGTATTCAGAACAATATTTTCATTTGCTAATGCCAGTGAACAGACTTTGGAAATTATCCCGAAACGGGTTCGTTTCGCGCAACCATTACCGTTAAACGTTCTCCGGGAGGTTGACAGc                    >  2:369649/1‑149 (MQ=255)                                                                                                                                 cTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGaa                                                                                                  <  1:333571/71‑1 (MQ=255)                                                                                                                                 cTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGaa                                                                                                  >  2:333571/1‑71 (MQ=255)                                                                                                                                 cTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGc                    >  2:449313/1‑149 (MQ=255)                                                                                                                                       gAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgttct              >  2:207709/1‑149 (MQ=255)                                                                                                                                        aaCCGTCCCTGTTTTGTTCTGAAGCGTATTCAGAACAATACTTTCCGTTGCGAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTTGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgttctg             <  1:131862/149‑1 (MQ=255)                                                                                                                                            gCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGGTATCAGGTATGTTTCAAGCTGTTCTGTTgg         >  1:83722/1‑149 (MQ=255)                                                                                                                                            gCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTgg         >  1:18276/1‑149 (MQ=255)                                                                                                                                              aacTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGc       <  2:424729/147‑1 (MQ=255)                                                                                                                                                  gTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCgg                                                            <  1:468564/92‑1 (MQ=255)                                                                                                                                                  gTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCgg                                                            >  2:468564/1‑92 (MQ=255)                                                                                                                                                   ttttGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGGCACCGTTACCGTTAAACGTTATCAGGTATGTTTTAAGCTGTTAGGTTGTGCCATaa  >  1:79630/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATAC  >  NC_000913_3_pae_tpiA/1570385‑1570679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.