New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 226785927 (0.960)4 (0.150) 4/268 3.4 13.4% coding (31/987 nt) yeiR Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein
?NC_000913_3_pae_tpiA = 2267869 26 (0.970)coding (41/987 nt) yeiR Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAA  >  NC_000913_3_pae_tpiA/2267712‑2267999
                                                                                                                                                   |                                                                                                                                            
cGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtct                                                                                                                                             <  2:326464/149‑1 (MQ=255)
          ggTTAAAGCGGATGAAGCTCACCTTCGTCCCCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCggg                                                                                                                                   <  1:60706/149‑1 (MQ=255)
                                              cccTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATaaa                                                                                               <  2:496046/149‑1 (MQ=255)
                                                    tctTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGATAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATccc                                                                                         >  2:18879/1‑149 (MQ=255)
                                                            gCCTTTTTTCGCCAGGATTAACGCTCAGTTAACTTACCAGAGTAGTGCGGAGCCGTCATGACCAGGACCAACCTGATCACCGGTTTTCTCGGCAGCGGGAAAACCCCTTCGATTCTTCATCTGGTAACCCATAAAAATACCacacaaac                                                                                 >  2:121292/1‑142 (MQ=255)
                                                             ccTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAGAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaaa                                                                                >  1:188849/1‑149 (MQ=255)
                                                                       ccAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTAc                                                                      >  2:27312/1‑149 (MQ=255)
                                                                        cAGGATTAACGCTCAGTTAACTTACCAGCGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGtt                                                                                                         >  2:109659/1‑113 (MQ=255)
                                                                        cAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGtt                                                                                                         <  1:109659/113‑1 (MQ=255)
                                                                          ggATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTgg                                                                   <  2:249535/149‑1 (MQ=255)
                                                                          ggATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCAGCACCGGTTTTCTCGGCAGCGGGAAATCCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTgg                                                                   <  1:84783/149‑1 (MQ=255)
                                                                          ggATTAACGCTCAGTTAACTTACCAGAGCATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTgg                                                                   <  1:40362/149‑1 (MQ=255)
                                                                           gATTAACGCTCAGTTAACTTACCAGAGGATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGt                                                                  <  1:24579/149‑1 (MQ=255)
                                                                                  gCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGCCCAACCTCATCACCAGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAACCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGaa                                                           >  1:219453/1‑149 (MQ=255)
                                                                                               aCCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCg                                              >  1:224464/1‑149 (MQ=255)
                                                                                                 cAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGATAATGAATTTGGGGAAGTCGGa                                            >  1:256514/1‑149 (MQ=255)
                                                                                                   gagTATTGCGAAGCCGGTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAAt                                          <  2:435668/149‑1 (MQ=255)
                                                                                                                   ttGTGACCAGGACCGACCTCATCAACGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTc                          >  1:432955/1‑149 (MQ=255)
                                                                                                                     gtgACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGc                        <  1:269483/149‑1 (MQ=255)
                                                                                                                               ccAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgg                                                      <  1:347438/109‑1 (MQ=255)
                                                                                                                               ccAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgg                                                      >  2:347438/1‑109 (MQ=255)
                                                                                                                               ccAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGcc                       <  1:23590/140‑1 (MQ=255)
                                                                                                                               ccAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGcc                       >  2:23590/1‑140 (MQ=255)
                                                                                                                                      catccCCGGTTTTCGCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGc       <  1:370900/149‑1 (MQ=255)
                                                                                                                                      catcaCCGGTTTTCTCGGCAGCGGGAAAAGCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACAAAAAATGGGCGGTACTGGTCAATGGATTTGGGGCAGTCGGAATTGATGGTGCTTTGCTCGCCAATAGCGCCGCATTGc       >  1:13818/1‑149 (MQ=255)
                                                                                                                                           ccGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGCATATCTGTAAGGCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGaaa  >  1:468433/1‑149 (MQ=255)
                                                                                                                                           ccGGTTTTCTCGCCAGCGGGAAAACCACGTCGAGTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGaaa  <  1:109624/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
CGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAA  >  NC_000913_3_pae_tpiA/2267712‑2267999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.