Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 3,440,923 C→T G36D (GGC→GAC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,440,9230CT97.7% 397.9 / NA 128G36D (GGC→GAC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (71/54);  minor base G (1/2);  total (72/56)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAA  >  NC_000913_3_pae_tpiA/3440777‑3441067
                                                                                                                                                  |                                                                                                                                                 
ggTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCa                                                                                                                                                 >  2:861139/1‑149 (MQ=255)
 gTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCaa                                                                                                                                                >  1:1440349/1‑149 (MQ=255)
 gTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCaa                                                                                                                                                >  1:795226/1‑149 (MQ=255)
     aGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAACGTATGGTCAAAGcc                                                                                                                                            >  1:1360192/1‑149 (MQ=255)
       cAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCAc                                                                                                                                          >  2:234739/1‑149 (MQ=255)
        aGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACg                                                                                                                                         >  1:544616/1‑149 (MQ=255)
            tttCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGctct                                                                                                                                     >  2:456930/1‑149 (MQ=255)
                cAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAg                                                                                                                                 >  2:772017/1‑149 (MQ=255)
                  ggATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGc                                                                                                                               <  1:456930/149‑1 (MQ=255)
                  ggATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGc                                                                                                                               <  1:1257329/149‑1 (MQ=255)
                       tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCCACCCGGCATCGATGCGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCACCGCCCTAACGGCTCacg                                                                                                                          >  2:166295/1‑147 (MQ=255)
                       tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCCGAGTATGGTCAAAGCCACGCTCTAAAGGCTCagg                                                                                                                          >  1:1156884/1‑147 (MQ=255)
                         ttCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAggg                                                                                                                        >  2:1147/1‑149 (MQ=255)
                           ccTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTCCACCATCAATCTCAGCCTCGGTCACCGTGCAGCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCGGCGTATGGTCAAAGGCACGCTCTAAAAGCTCTAggggc                                                                                                                      >  2:1033372/1‑147 (MQ=255)
                           ccTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                      <  2:544616/149‑1 (MQ=255)
                             tGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGCTGAGATCAGAATACGAAATCGTGTGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCAc                                                                                                                    >  1:273538/1‑149 (MQ=255)
                              gAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCAcc                                                                                                                   <  1:1147/149‑1 (MQ=255)
                               aaCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCGAGGGTCCCCt                                                                                                                  >  1:908286/1‑149 (MQ=255)
                                  gCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCCCCACGAAACTGGCCACCGATGAGACTGGATTACGTCGCAGTGCGTTACCCAGAGGATGGTCAAAGCCAGGCCCAAAAGGCTCAAGGGACACCTTgg                                                                                                               >  1:1065633/1‑149 (MQ=255)
                                    cttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCCCGCTCTAACGGCTCAAGGGTCACCTTGgcg                                                                                                             >  1:30249/1‑149 (MQ=255)
                                    cttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACGTTGgcg                                                                                                             >  2:1432045/1‑149 (MQ=255)
                                       cttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAAGACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCGTGgcgtgc                                                                                                          <  1:568296/149‑1 (MQ=255)
                                        tttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgcg                                                                                                         >  1:661203/1‑149 (MQ=255)
                                        tttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCGCTAAAGGCTCAAGGGTCACCTTGgcgtgcg                                                                                                         >  1:450173/1‑149 (MQ=255)
                                             tGCTGTACTCATGTAGTACACCATCACTCGCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                                    <  2:520527/149‑1 (MQ=255)
                                             tGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                                    <  1:234739/149‑1 (MQ=255)
                                             ggCTGGACTCATGTAGTACATCATCGATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATAAGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                                    <  2:728262/148‑1 (MQ=255)
                                              gCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaaa                                                                                                   >  1:546964/1‑148 (MQ=255)
                                                 gTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTTACCTTGGCGTGCGTCGAACTCa                                                                                                >  1:1029639/1‑149 (MQ=255)
                                                  tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAc                                                                                               <  2:727167/149‑1 (MQ=255)
                                                  tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAc                                                                                               >  2:302067/1‑149 (MQ=255)
                                                    cTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCACAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACtt                                                                                             >  2:1283670/1‑149 (MQ=255)
                                                     tCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTCTGGTCAGAGCCACGCTCGAAAGGCCCAAGGGCCACCTTGCCGTGCGTCGACCTCCCtcg                                                                                            >  2:151565/1‑147 (MQ=255)
                                                       ctgtgGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                         >  2:1097798/2‑149 (MQ=255)
                                                       aTGTAGTACACCATCAATCTCACCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCCGTGCGTTACCCAGAGTCTGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGGGCGTCGCACTCACTTACt                                                                                         >  2:717334/1‑149 (MQ=255)
                                                         gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCg                                                                                       >  1:179329/1‑149 (MQ=255)
                                                          tagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCCCTAAAGGCCCAAGGGTCACCTTGGCGTGCGTCGAACTCCCTTCCTcca                                                                                      >  1:545809/1‑147 (MQ=255)
                                                           agtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGat                                                                                     <  1:1263801/149‑1 (MQ=255)
                                                             taCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                   >  1:1045630/1‑149 (MQ=255)
                                                                  cATCAATCTCAACCTCGGTCACCGCGCCACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAcc                                                                              <  1:384777/149‑1 (MQ=255)
                                                                  cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAcc                                                                              >  1:897635/1‑149 (MQ=255)
                                                                   aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                             <  2:179329/149‑1 (MQ=255)
                                                                   aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCCAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATccacca                                                                             >  2:18235/1‑149 (MQ=255)
                                                                   aTCAATCTCAACCTAGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                             >  1:1251283/1‑149 (MQ=255)
                                                                        tctcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGacgc                                                                        >  1:1083212/1‑149 (MQ=255)
                                                                          tcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGc                                                                                                                               >  1:1337042/1‑93 (MQ=255)
                                                                          tcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGc                                                                                                                               <  2:1337042/93‑1 (MQ=255)
                                                                           caccgtCGGTACCCGGGCAACCCGGCATCGATACGAGCCGAATCCGGTCCCGTGCGTTCCCCAGAGTATCGTCAATCCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCCTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGt                                                                     <  2:829113/144‑1 (MQ=255)
                                                                             aCCTCGGTGACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGttt                                                                   <  2:917903/149‑1 (MQ=255)
                                                                                tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAg                                                                >  1:1113196/1‑149 (MQ=255)
                                                                                tCGGGCACCGCGTAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCCCGCTCAAAAGGCTCAGAGGTCACCGTGGCGTGCCTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAg                                                                <  2:888090/149‑1 (MQ=255)
                                                                                  cgTCACCGCGCAGCCCGGCATCGAGGAGAGCAGAATAAGGCGCAGTGCGTTCCCCAGAGTATGGTCAAAGACACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGaa                                                              <  2:303411/148‑1 (MQ=255)
                                                                                       ccGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                         >  1:1005365/1‑149 (MQ=255)
                                                                                         gggccaCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGt                                                       <  2:187471/144‑1 (MQ=255)
                                                                                         gcgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCGCAAGGGTCACCTTGGGTGGCGGCTAACTCGCTTGCTCGGTATTAAGCAGGCGCGAGTTTAGAAActcggg                                                       >  1:1382945/1‑146 (MQ=255)
                                                                                          cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTc                                                      <  1:1027376/149‑1 (MQ=255)
                                                                                             aaCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                   <  1:481214/149‑1 (MQ=255)
                                                                                               tccGGCATCGAGGAGAGCAGAATACGGCGCAGTGCGCTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCATTTTCTCGATATCGACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                                 <  1:1144163/148‑1 (MQ=255)
                                                                                               cccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                                 >  1:1058204/1‑149 (MQ=255)
                                                                                                ccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGaa                                                >  2:491899/1‑149 (MQ=255)
                                                                                                 cGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTa                                                                 >  1:1291241/1‑131 (MQ=255)
                                                                                                 cGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTa                                                                 <  2:1291241/131‑1 (MQ=255)
                                                                                                    caacGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCt                                            <  1:302067/146‑1 (MQ=255)
                                                                                                    cATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTTACTGAACCCt                                            >  1:58672/1‑149 (MQ=255)
                                                                                                    cATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCt                                            <  2:1440349/149‑1 (MQ=255)
                                                                                                       cGATGAGAGCAGAATACGGCGCAGTGCGTTACCATGAGTATGGTTGTCGCCGAAGAAACCGTACTCGAGGGTTAACTTGGAGTGCGTCGAAGTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCa                                         <  2:1029639/149‑1 (MQ=255)
                                                                                                          tGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCCCCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCGCAGAACCCTGCATtg                                      >  1:692645/1‑149 (MQ=255)
                                                                                                            agagCAGAATACGGCGCAGTGCGTTACCCAGATTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgtg                                    <  2:1112401/149‑1 (MQ=255)
                                                                                                              agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCCCTTGCCCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                  <  1:772017/149‑1 (MQ=255)
                                                                                                              agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                  >  1:164959/1‑149 (MQ=255)
                                                                                                              agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                  <  2:1045630/149‑1 (MQ=255)
                                                                                                                cAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                <  2:1001357/149‑1 (MQ=255)
                                                                                                                 aGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctc                               >  2:353365/1‑149 (MQ=255)
                                                                                                                 aGAATACGGCGCAGGGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctc                               <  2:795226/149‑1 (MQ=255)
                                                                                                                     tACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTATCCTCTCtt                           >  2:876584/1‑149 (MQ=255)
                                                                                                                     tACGGCGCAGTACGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCCTTGTGTCCTCTCtt                           >  1:886302/1‑149 (MQ=255)
                                                                                                                      aCGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCGCTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                          <  1:1102093/149‑1 (MQ=255)
                                                                                                                      aCGGCGCAGTGCGTTACCCAGAGTACGGGCATAGCCACGCCCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                          <  1:1194949/149‑1 (MQ=255)
                                                                                                                        ggCGCAGTGCCTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTTACAGAACCCTGCATTGTGTCCTCTCTTTgg                        <  1:857066/149‑1 (MQ=255)
                                                                                                                         gcgcAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGt                       <  2:407066/149‑1 (MQ=255)
                                                                                                                               tGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCTCGGTTTTAGAAACTCTGTCACAGCACCCTGCATTGTCTCCTCTCTTTGGTACTAAg                 >  2:618035/1‑149 (MQ=255)
                                                                                                                               tGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAg                 >  1:721916/1‑149 (MQ=255)
                                                                                                                               tGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCCCTTTGGTACtcat                 >  2:492419/1‑146 (MQ=255)
                                                                                                                                gCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGc                <  2:995761/149‑1 (MQ=255)
                                                                                                                                gCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACATGGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGc                >  1:1005498/1‑149 (MQ=255)
                                                                                                                                gCGTGACGCAGAGTATTGGTAAGGTCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGc                <  2:466306/149‑1 (MQ=255)
                                                                                                                                 cGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGACCCCTGCCTTGTGTCTTCCCTTGGGTACTAAgat               >  1:1211499/1‑147 (MQ=255)
                                                                                                                                  gttgcgcAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACGCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCtt              <  2:1288839/143‑1 (MQ=255)
                                                                                                                                   ttACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCttt             >  2:733390/1‑149 (MQ=255)
                                                                                                                                   ttACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATCGTGTCCTCTCTTTGGTACTAAGCttt             >  2:954423/1‑149 (MQ=255)
                                                                                                                                      cccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGt                       >  2:571051/1‑136 (MQ=255)
                                                                                                                                       ccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCCCAGAACCCTGCCTTGTGTCCTCTCTTTGGTACTAAGCtttcatt         >  1:583571/1‑145 (MQ=255)
                                                                                                                                        cAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAACGTTAAACATTTTGTCCTCTCTTTGGTACTAAGCTTTACTTg        <  1:708508/149‑1 (MQ=255)
                                                                                                                                          gagTATGGTCAAAGCCACGGTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa      <  2:516604/149‑1 (MQ=255)
                                                                                                                                          gagTATGGTCAAAGCCACGCTCTTAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa      <  2:1020908/149‑1 (MQ=255)
                                                                                                                                          gagTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCTTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa      <  1:130133/149‑1 (MQ=255)
                                                                                                                                          gagTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa      <  1:18235/149‑1 (MQ=255)
                                                                                                                                             tATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                         >  1:801532/1‑95 (MQ=255)
                                                                                                                                             tATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                         <  2:801532/95‑1 (MQ=255)
                                                                                                                                              aTGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAATTGCCGCGAATCAACCACGCTATGGGTGAGAAACTCTGTCACAGAACCCGCCATTGTGTCCTCTCTCTGGTACTAAGCTTTACTTGGAggca  >  1:1421751/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAA  >  NC_000913_3_pae_tpiA/3440777‑3441067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: