New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 303629	28 (0.850)	4 (0.130)	4/260	4.1	12.8%	intergenic (+24/+224)	yagU/ykgJ	DUF1440 family inner membrane acid resistance protein/UPF0153 cysteine cluster protein
	?	NC_000913_3_pae_tpiA	= 303636	29 (0.970)					intergenic (+31/+217)	yagU/ykgJ	DUF1440 family inner membrane acid resistance protein/UPF0153 cysteine cluster protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCAAATAGAAGAAGTAT  >  NC_000913_3_pae_tpiA/303505‑303778                                                                                                                                    |                                                                                                                                               aTTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTtatcagt                                                                                                                               >  2:518098/1‑145 (MQ=255)   ttAGTCTGGTTCTGGTCTATTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTa                                                                                                                             >  2:168099/1‑149 (MQ=255)     aGTCTGGTTCTGTTCTATTGAAATTATTCGTAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTaaa                                                                                                                           <  2:99381/149‑1 (MQ=255)           gttctggtCTATTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACAtt                                                                                                                     <  1:556431/149‑1 (MQ=255)           gttctggtCTATTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACAtt                                                                                                                     >  2:189669/1‑149 (MQ=255)                 gtCTATTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCCACAATACAACGCCATTTTTATAGGTTCAAAACATTGCtttt                                                                                                               >  2:81486/1‑149 (MQ=255)                     aTTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTtata                                                                                                           <  2:303417/149‑1 (MQ=255)                     aTTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAAATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTGTtata                                                                                                           >  1:161939/1‑149 (MQ=255)                            ttattCGCAGAGATTTACGAAACAGAATTACTCATTAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGAt                                                                                                    <  1:105424/149‑1 (MQ=255)                            ttattCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGAt                                                                                                    >  2:105424/1‑149 (MQ=255)                             tattCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATg                                                                                                   >  1:463418/1‑149 (MQ=255)                                  gCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGAt                                                                                              >  2:121632/1‑149 (MQ=255)                                   cAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACa                                                                                                                       >  1:345469/1‑123 (MQ=255)                                   cAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACa                                                                                                                       <  2:345469/123‑1 (MQ=255)                                   cAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATa                                                                                             <  1:75666/149‑1 (MQ=255)                                       ggttcaCGAACTAGAATTATCCATGAGCTAGAGCCTTAGATCCCTGTAGGCTCAAACAGATAATGCATTGAATGATAAAAATCGCGCAAATGCAGCGCCATTTTTATAGGTTAAAAACATTGCTTCTTATATTCTGTTGCAGATAGTCa                                                                                         <  2:29634/144‑1 (MQ=255)                                                   aggaTTACTAATGAGCCAGACCCTGAGATCCCTTTAGGCTAAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATcg                                                                             <  2:567927/146‑1 (MQ=255)                                                           tCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACtt                                                                     >  2:31374/1‑149 (MQ=255)                                                           tCATGAGCCAGACCCTGAGATCCCTTTAGGATCACCATGATAAGGCATTGAATGATAAAAATGGCGCAAGTACAGCTCCATTTTTATAAGTTCAACGCATAGGTAATGAGTGTCGGATGAAGATAGTCAGTGAGTATATCGCGCTACtt                                                                     >  2:288537/1‑149 (MQ=255)                                                            cATGAGCCAGTCCCTGAGATCCCTTTAGGCTAAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTc                                                                    <  1:287002/149‑1 (MQ=255)                                                             aTGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATGGCGCTCCTTCa                                                                   >  2:253918/1‑149 (MQ=255)                                                              tGAGCCAGATCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGCCAGTGAGTATATCGCGCTACttacg                                                                  >  1:467652/1‑146 (MQ=255)                                                                    aGACCTTTAGATCCGTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCACATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGgatgat                                                            <  2:352546/149‑1 (MQ=255)                                                                     gACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGatgatg                                                           <  2:336095/149‑1 (MQ=255)                                                                        ccTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAg                                                        >  1:9589/1‑149 (MQ=255)                                                                                          tCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACag                                      <  1:347524/149‑1 (MQ=255)                                                                                                      tGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGCGGCCtt                          >  2:178157/1‑149 (MQ=255)                                                                                                                  tAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCaaa              >  1:397298/1‑149 (MQ=255)                                                                                                                     aaaTGGCGCAAATACACGGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCAAATag           <  1:459142/149‑1 (MQ=255)                                                                                                                              aaaTACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCCCTACTTCAGGATGACGTAGATCCGAAGAACGCTAAAGAAGAGAGCAATAGTTCCTGGCAAATAGAAGAAGTAt  >  1:90379/1‑149 (MQ=255)                                                                                                                                    |                                                                                                                                               ATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTAGGCTCAAACAGATAATGCATTGAATGATAAAAATGGCGCAAATACAGCGCCATTTTTATAGGTTAAAAACATTGCTTTTTATATTCTGATGCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCAAATAGAAGAAGTAT  >  NC_000913_3_pae_tpiA/303505‑303778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.