Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	264,098	0	G	T	95.8%	66.1 / NA	25	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (15/8);  total (16/9)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTA  >  NC_000913_3_pae_tpiA/263957‑264233                                                                                                                                              |                                                                                                                                        tCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCCTTACAGTTGTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTc                                                                                                                                  >  1:256195/1‑149 (MQ=255)   ccAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTAAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTGCTGGCGGCGTTCGGCGTATACCGCTGGGACTCCg                                                                                                                                >  1:42979/1‑149 (MQ=255)     aGGCGCAGCGGAATCTCCCTCTGCAGGCCCCTGGAAGGGATGCTCATCACAGTTCGCCACTATTGTCACTTCAGGGTGATTCTCAACAGGCAGGTATAGCGCCCATTGCGGTCCTGGCGGCGGTCGGCGTATACCGCTAGGACTCCGGc                                                                                                                              <  1:191637/149‑1 (MQ=255)                   ctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                                                                                                >  1:6011/1‑149 (MQ=255)                       cGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCCGAACGTCCCt                                                                                                            >  2:272318/1‑149 (MQ=255)                           gCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCg                                                                                                        <  2:158439/149‑1 (MQ=255)                                  ccTGGTGGGGTTGCGTATTAGAGTTCTCCACTAGTGTCGGTTCAGGGTGATGCTGATCAGGCGGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGGCTCTGGCGATATCCGGATCGTCCCTGCCGGTGTAAt                                                                                                 <  2:564632/149‑1 (MQ=255)                                         gggTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc                                                                                          <  1:35823/149‑1 (MQ=255)                                                   ttACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGCGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGc                                                                                >  1:377781/1‑149 (MQ=255)                                                     aCAGTTTGCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCa                                                                              <  2:348830/149‑1 (MQ=255)                                                            tcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCCCTGGTAt                                                                       >  1:63350/1‑149 (MQ=255)                                                               aCTATCGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAGGCTACCGTGCCACTGGTATTTg                                                                    >  2:498117/1‑149 (MQ=255)                                                                      tCAGTTCAGGGGGATGCTCATCAGGCGGGGATAGGGCCCATTGCAGTCCTGGCGGCGTTCTGCGTATACCGCCAGGCCTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGCCGCTACCGTGCCCCTGGTATTTGCCGGTGc                                                             >  2:98538/1‑149 (MQ=255)                                                                                       tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                            >  1:389948/1‑149 (MQ=255)                                                                                       tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACGGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                            >  1:292836/1‑149 (MQ=255)                                                                                         atcaGGCAGGTATAGGGCCCATTGCGGTCCTAGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGa                                          >  2:368453/1‑149 (MQ=255)                                                                                              gcaggTATAGGGCCCGTTGCGGTCCTGACGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATGTCCGGAACGTCCCTGCCGGTGCAATGACAGACGCTACCGTGCCACTGGGCTTTGCCGGTGCGGTAGCCAAAGAATCggggcccac                                     >  2:54013/1‑143 (MQ=18)                                                                                              gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGCCTCAg                                     >  1:85481/1‑149 (MQ=255)                                                                                                        ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACCCAGGATGcgagcg                           >  2:397848/1‑144 (MQ=255)                                                                                                               ttcGGTCGTGTTGGCGGTCGGCGTATAGCGCTAGGACTCCGGGGAGATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATc                    <  1:89971/147‑1 (MQ=14)                                                                                                                     cctagcggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCAtt              <  1:368453/145‑1 (MQ=255)                                                                                                                           ggcgTTCGGCGTATACCGCTAGGACTCCGGCTATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTTCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTg        >  1:199339/1‑149 (MQ=255)                                                                                                                           ggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTg        >  1:150180/1‑149 (MQ=255)                                                                                                                              gtccGGCGTAGACCGTGAGGACTCCGGCGATATCGTGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGt     <  1:344592/146‑1 (MQ=17)                                                                                                                                 cGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTa  <  1:528097/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                        TCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTA  >  NC_000913_3_pae_tpiA/263957‑264233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑