New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 4400048	39 (1.070)	4 (0.120)	4/272	4.7	9.3%	coding (881/951 nt)	miaA	delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase
	?	NC_000913_3_pae_tpiA	= 4400054	41 (1.180)					coding (887/951 nt)	miaA	delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGA  >  NC_000913_3_pae_tpiA/4399908‑4400199                                                                                                                                                   |                                                                                                                                                  agtgtggggTTATCGCTAGAGGTGGTCTTGCCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATTGAGGTGGTTGCGCCACGGGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACa                                                                                                                                                 <  2:337254/143‑1 (MQ=255)      tGGGGTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTAGCGAAGCGGCAGATAACCTGGCTGCATGGTGAGGAAGGGGTTCACTGGATTGACAGTGac                                                                                                                                            >  2:170809/1‑148 (MQ=255)                      gtgGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGgcgc                                                                                                                            <  2:431399/149‑1 (MQ=255)                        ggTCTTTCCTTGAAGGCGAAATCTCATACGATGAAAGGGTTTATCGAGGTGTTTGCGCCACGAGACAGTGGGCGAAGCGGCAGATAACCAGCCTGCGTTGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGt                                                                                                                          <  2:588437/149‑1 (MQ=255)                        ggTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGt                                                                                                                          <  1:559298/149‑1 (MQ=255)                             tACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGGCAGTTAAAAACCAGAACAGGCGCAGGACGa                                                                                                                     >  1:221749/1‑149 (MQ=255)                                    aaGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTAGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTa                                                                                                              <  1:287526/149‑1 (MQ=255)                                       gCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAg                                                                                                           >  1:104027/1‑149 (MQ=255)                                              ttgaTACAATGAAATGTGTTATCTAGGTGTTTGCGCCACGAGACAGTTTGCGGAGGCGCAGATAAGCTGGCTGCGTGGTTGTGAAGGGGTTCACTGTCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGttgttg                                                                                                    <  2:363386/146‑1 (MQ=255)                                              ctcATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGttgttg                                                                                                    <  2:472976/149‑1 (MQ=255)                                              ctcATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGgtgttg                                                                                                    <  2:59243/149‑1 (MQ=255)                                                caaatgATGAAATTGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGt                                                                                                  <  2:397726/144‑1 (MQ=255)                                                 aTACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGTGTGTTTGtt                                                                                                 >  2:489997/1‑148 (MQ=255)                                                          aaTGGTTTATCGAGGTGTTTGCGCCACGGGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGTAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAg                                                                                        <  1:423925/149‑1 (MQ=255)                                                               tttATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCGGAACAGGCGCGGGACGAAGTATTACAGGTTGTAGGTGCTATCGCAGGCTgg                                                                                   >  2:513338/1‑148 (MQ=255)                                                                 tATCGAGGTGTTTGCTCCACGAGACAGTTGGCCAAGCTGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAAt                                                                                 <  2:564667/149‑1 (MQ=255)                                                                    cGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAAtgtg                                                                              <  1:109734/149‑1 (MQ=255)                                                                      aGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTa                                                                            <  2:16428/149‑1 (MQ=255)                                                                         tgtTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGCGACGTAGTGGTACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACaa                                                                         >  1:445382/1‑149 (MQ=255)                                                                           tttGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTAt                                                                                             <  1:384548/127‑1 (MQ=255)                                                                           tttGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTAt                                                                                             >  2:384548/1‑127 (MQ=255)                                                                             tGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTga                                                                     <  2:461138/149‑1 (MQ=255)                                                                                   aCGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTAGGGAAGGGGTTCACTGGCTTGAGAGTGAAAAACCAGAACAGGCGCGTAACGAAATATTACAGGTTGTTGGTCCTATCGAAGGCTGGATCTGTCGAATTGATATGTa                                                               >  2:120484/1‑149 (MQ=255)                                                                                           gTTGGCGAAGCGGCAGATACCCTGGCTGCGTGGTTGGGAAGAGGTTCACTGGCTTGACAGTG‑AAGACCTGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTACTATCCCAGGCTGACTGTGTCCAATTGAGACGTATCGTgcgcc                                                      >  2:578515/1‑148 (MQ=255)                                                                                               gCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAAtt                                                   >  1:432179/1‑149 (MQ=255)                                                                                               gCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGGTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGCCGAAGTATTACAGGTTGTTGGTGCTATCACAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAAtt                                                   >  1:638/1‑149 (MQ=255)                                                                                                    gCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGCCGAAGTATTACAGGTTGTTGGTGCTATCGCAAGCTTAATGTGTTCAATTACTACGTATCTTTCGCAATTTTTTc                                              >  2:371153/1‑149 (MQ=255)                                                                                                        cAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGaa                                          <  1:583921/149‑1 (MQ=255)                                                                                                         aGATAACCTGGCTGGGTGGTGGGGTAGGGGTTCAATGGCTGGACAGTGAAAAACCAGAACAGGAGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTCCAGAAt                                         <  2:148975/149‑1 (MQ=255)                                                                                                                cTGGCTGCGGGGTTGGGGAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGTTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAgg                                  <  2:44643/149‑1 (MQ=255)                                                                                                                          ggTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTAc                        <  1:493138/149‑1 (MQ=255)                                                                                                                               ggAAGTGGATCACAGGCTTTACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGCACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATa                   >  2:559589/1‑149 (MQ=255)                                                                                                                               ggAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAgg                                                                                                          <  1:126886/62‑1 (MQ=255)                                                                                                                               ggAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAgg                                                                                                          >  2:126886/1‑62 (MQ=255)                                                                                                                                gAAGGGGTTGACGGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATaa                  <  2:445382/149‑1 (MQ=255)                                                                                                                                 aaGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACCTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAg                 >  1:601803/1‑149 (MQ=255)                                                                                                                                       ttCACTGGCTTGACAGTGAAAAAACAGAACAGGCGCGTGACGAAGTATTAAAGATTGATGGTGATAACACAGGCATAATGTGTACAATCGAGACGTCGCGTGCGCCATTTTGTCAGAAACGACAGGTTCAAAGTACAAAGAAACAtata           >  2:554144/1‑149 (MQ=255)                                                                                                                                          aCTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGATGCTATCGCAGGCTGGATGTGTACAATTGAGAAGTAGCGTACGCACTTTTTTCAAAATCGACACGATCAAAGTACAACTAAGGATATCAgg        >  2:616601/1‑149 (MQ=255)                                                                                                                                            tGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATGGCAGGCTGAATGTGTACAATTGAGACGGAGCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGaa      >  1:299827/1‑149 (MQ=255)                                                                                                                                              gCTTGAAAGGGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGaaaa    <  2:250031/149‑1 (MQ=255)                                                                                                                                                ttGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAaga  >  1:80062/1‑149 (MQ=255)                                                                                                                                                   aCAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGtt                                >  2:367842/1‑116 (MQ=255)                                                                                                                                                   aCAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGtt                                <  1:367842/116‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGA  >  NC_000913_3_pae_tpiA/4399908‑4400199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.