New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 460951433 (0.910)4 (0.120) 4/270 4.6 11.7% coding (185/1590 nt) prfC peptide chain release factor RF‑3
?NC_000913_3_pae_tpiA = 4609530 29 (0.840)coding (201/1590 nt) prfC peptide chain release factor RF‑3
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTATTTCTCACCCGGACGCCGGTAAGACTACCATCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTA  >  NC_000913_3_pae_tpiA/4609382‑4609666
                                                                                                                                                    |                                                                                                                                        
ttattTCTCACCCGGACGCCAGTAAGACTACCATCACCGAGAAGGGGCTGCGGCTGGGACATAAAGTGCAGAGCACCGGGAAGGTAAAGGGACGTGGGGACACCGAGCACACTAATTCGGACTCGATGGCGATGGACAAGCAGCGTggg                                                                                                                                          >  2:510074/1‑149 (MQ=255)
ttagtTTTCACCCGGACGGCGGGAAGACTACCATCACCGAGAAGGTGCTGCTGTTCTGAAAGGTCATTCAGAACGCCGGTACAGTAAAGGGCCGTGGTTGCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTggg                                                                                                                                          <  1:205233/149‑1 (MQ=255)
gtggttCTCACCCGGACGTCGGTAAGACTACCAGCACCGAGAAGGTGCTGCTGTTGGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGGACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTggg                                                                                                                                          <  2:259286/145‑1 (MQ=255)
         aCCCGGACGCCGGTAAGACTACCATCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCAtt                                                                                                                                 >  1:400235/1‑149 (MQ=255)
           ccGGACGCCGGTAAGACTACCATCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATtac                                                                                                                               >  1:77863/1‑149 (MQ=255)
             ggACGCCGGTAAGACTACCATCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATtacta                                                                                                                             <  2:94410/149‑1 (MQ=255)
                    ggTAAGACTCCCCGCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTTGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCtgt                                                                                                                      <  2:284053/149‑1 (MQ=255)
                     gTAAGACTACCATCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCtgtg                                                                                                                     <  1:59803/149‑1 (MQ=255)
                                 tCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCTGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCg                                                                                                         >  1:573233/1‑149 (MQ=255)
                                   aCCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTa                                                                                                       <  2:573233/149‑1 (MQ=255)
                                                tgctgTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGCTTCCCTg                                                                                          >  1:453072/1‑149 (MQ=255)
                                                tgctgTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTACGTATCACGATTGCCTg                                                                                          >  1:434889/1‑149 (MQ=255)
                                                 gctgTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTgg                                                                                         >  1:270270/1‑149 (MQ=255)
                                                     ttCGGAGAGGCCATTGAGGCCGCGGGTACAGTCAGAGGCGGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTGCCGTAGCACGATTGCCTGGTTaa                                                                                     <  2:205912/149‑1 (MQ=255)
                                                        ggACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCt                                                                                  <  2:77863/149‑1 (MQ=255)
                                                              gCCATTCAGACCGCCGGTACAGTAAAAGGCAGTGGTTCCAACGAGCACGCTAAGGCGGACTGGAGGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATACCGATTGCCTGGTTAACCTGGTCGa                                                                            >  2:635607/1‑149 (MQ=255)
                                                               ccATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGac                                                                           >  1:430580/1‑149 (MQ=255)
                                                                       aCCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCggg                                                                   <  2:400235/149‑1 (MQ=255)
                                                                       aCCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCggg                                                                   <  1:544892/149‑1 (MQ=255)
                                                                                 cAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGAc                                                         <  2:148149/149‑1 (MQ=255)
                                                                                   gTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGAcgt                                                       <  2:563713/149‑3 (MQ=255)
                                                                                   gTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCCCCATTACTACGTCTGTGATGAAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCCGGGCACGAAGACtt                                                       >  2:172661/1‑149 (MQ=255)
                                                                                          gCCGGGGCTACAACCAGCACGATGAGTCGAACGCGAAGGAAATGCAAAAGAAGCGGACGATCTCCAGCACCACAGCGGTGCTGGAGTTCACGGACCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACCTCTCGGaa                                                <  2:18132/149‑1 (MQ=255)
                                                                                              ttgTTCCAACCAGCACGCAAAGTAGGGCCGGAGGGAGATGGAAACGCTCCGGGGTAGCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATa                                            <  2:103200/147‑1 (MQ=255)
                                                                                               ggTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTACGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATAc                                           <  2:434889/149‑1 (MQ=255)
                                                                                                                 aaGTCGGACTGGATGGAGATGGAAAAGCAGCATGGGATCTGCATTACTACGTCTGGGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCAGGGGCACGAAGACTTCTCGGCAGATACCTATCGTACCCTGAcggc                         >  2:441643/1‑149 (MQ=255)
                                                                                                                    tCGTACTGCATGGAGTTTGAAGAGCAGTGTGGGATCTCTATTACTACGTCTGTGATGCGGCTTACGCATCTCGATGGCCCCGTTAACCCGCTCGACACCCCGGGGCACGCAGACCTCTCGGTAGATACCTATCGTACCCTGACGGCGGt                      <  2:70125/149‑1 (MQ=255)
                                                                                                                       ggcgggaggggGATGGAAGAGCTGGGTGGGAAGTGTATTACTAAGTGTGTGATGCAGGCTCCGCATCACGATTGCCTGGTTAACCTGCTCGACGCCCCGGGGCACGAACCCTTCCTGGCAGATACCTATCTGATCCGGACGGCGGTGGa                   <  2:635582/145‑1 (MQ=255)
                                                                                                                                   atggaAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGa                                                                            >  1:621345/1‑80 (MQ=255)
                                                                                                                                   atggaAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGa                                                                            <  2:621345/80‑1 (MQ=255)
                                                                                                                                    tggaAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGACGCAGTTTCCGTATCACGATTGCCTGCTTAACCTGCTCGCCACCCCGGCGCACGAAGCCTTCTGGCAAGATACCCATCGTACCCTCCCGCCGGTGGCCTCCTCCatgatg      >  2:236824/1‑149 (MQ=255)
                                                                                                                                        aaaGCAGCGTGGGATCTCCATTACTACGTCGGTGATGCAGCTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGCTGAACTGCTGCCTGCTCGTTa  >  2:213298/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
TTATTTCTCACCCGGACGCCGGTAAGACTACCATCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTA  >  NC_000913_3_pae_tpiA/4609382‑4609666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.