Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,756,121 Δ557 bp coding (424‑980/1413 nt) pykF → pyruvate kinase I

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1756121 1756677 557 84 [0] [0] 86 pykF pyruvate kinase I

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 17561200 (0.000)84 (1.010) 68/284 0.2 100% coding (423/1413 nt) pykF pyruvate kinase I
?NC_000913_3_pae_tpiA 1756678 = 0 (0.000)coding (981/1413 nt) pykF pyruvate kinase I

CCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTG  >  NC_000913_3_pae_tpiA/1756530‑1756826
                                                                                                                                                    |                                                                                                                                                    
tttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttgccaacaccgtactggttgacgctggtctgatcggtatggaagttacagccactcaaggtaacaaac                                                                                                                                                      >  2:1427440‑M2/149‑149 (MQ=255)
 ttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaca                                                                                                                                                     <  2:1208583‑M2/2‑1 (MQ=255)
  tcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcgttatggaagttaccgccattgaaggtaacaaaCAt                                                                                                                                                    <  2:933375‑M2/3‑1 (MQ=255)
     ccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtcggatcggtctggaagttaccgccattgaaggtaacaaaCATGGc                                                                                                                                                 >  1:425639‑M2/144‑149 (MQ=255)
     ccacgcataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctggctgtgggcaacaccgtacttgttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGc                                                                                                                                                 <  1:56317‑M2/6‑1 (MQ=255)
       actgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGa                                                                                                                                               <  2:1402820‑M2/8‑1 (MQ=255)
       actgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGa                                                                                                                                               <  1:215998‑M2/8‑1 (MQ=255)
       actaataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGa                                                                                                                                               <  2:33534‑M2/8‑1 (MQ=255)
          gataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaacCATGGCGACCa                                                                                                                                            >  1:147350‑M2/139‑149 (MQ=255)
          gataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCa                                                                                                                                            >  2:578016‑M2/139‑149 (MQ=255)
           ataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCAt                                                                                                                                           >  1:1390379‑M2/138‑149 (MQ=255)
              aatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTg                                                                                                                                        <  1:605587‑M2/15‑1 (MQ=255)
              aatccgttaacggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTg                                                                                                                                        <  1:1032793‑M2/15‑1 (MQ=255)
                tctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCg                                                                                                                                      >  1:965389‑M2/133‑149 (MQ=255)
                tctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCg                                                                                                                                      >  1:493950‑M2/133‑149 (MQ=255)
                  tgttatcggcaacagcgaaagggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGaa                                                                                                                                    <  1:659678‑M2/19‑1 (MQ=255)
                   gttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgacattgaaggtaacaaaCATGGCGACCATCTGCGAAc                                                                                                                                   <  2:90495‑M2/20‑1 (MQ=255)
                       tcggcatcagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTac                                                                                                                               <  1:809069‑M2/24‑1 (MQ=255)
                       tcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGCCCATCTGCGAACGTac                                                                                                                               >  1:236784‑M2/126‑149 (MQ=255)
                       tcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTac                                                                                                                               >  1:1397678‑M2/126‑149 (MQ=255)
                        cgggaaaagcggaatggttggggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggtggacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTacc                                                                                                                              <  2:147350‑M2/25‑1 (MQ=255)
                          gcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTaccga                                                                                                                            >  1:1358596‑M2/123‑149 (MQ=255)
                                gcgaaatggttgcggtaacgtatgaaggtttcaatactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGt                                                                                                                      <  1:891449‑M2/33‑1 (MQ=255)
                                  gaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGtga                                                                                                                    <  1:936049‑M2/35‑1 (MQ=255)
                                  gaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGtga                                                                                                                    >  1:502650‑M2/115‑149 (MQ=255)
                                     atggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGtgatga                                                                                                                 <  1:845581‑M2/38‑1 (MQ=255)
                                      gggtggcggttacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACGATCTGCGAACGTACCGACCGCGTGATGaa                                                                                                                <  2:218632‑M2/39‑1 (MQ=255)
                                               taacgtatgaaggtttcactagtgacctgtctgttggcaccaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtct                                                                                                       <  2:1289211‑M2/48‑1 (MQ=255)
                                                aacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtctc                                                                                                      <  2:502650‑M2/49‑1 (MQ=255)
                                                    tatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGt                                                                                                  >  2:40123‑M2/97‑149 (MQ=255)
                                                      tgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttacccaacttgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTc                                                                                                <  1:927497‑M2/55‑1 (MQ=255)
                                                       gaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTca                                                                                               >  1:745067‑M2/94‑149 (MQ=255)
                                                       gaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTca                                                                                               >  1:1164259‑M2/94‑149 (MQ=255)
                                                       gaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTca                                                                                               >  1:1164319‑M2/94‑149 (MQ=255)
                                                          gttttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaaca                                                                                            <  2:499936‑M2/59‑1 (MQ=255)
                                                             ttcactactgacctgtctgttgggaaaaccgttctggttggcgatggtatgattgttattgaagttaccgccagtgaggggaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATg                                                                                         <  2:491274‑M2/62‑1 (MQ=255)
                                                              tcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGTCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTGAACaattc                                                                                        >  2:369705‑M2/87‑147 (MQ=255)
                                                                acgactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACa                                                                                      <  2:231921‑M2/65‑1 (MQ=255)
                                                                  tactgacctgtctgttggcaacaccgtactggttgacgctggtctgatcggtatggaagttaccgccatcgaaggtaacaaaCATGGCGACCATCTGCGAAGGTACCGACCGCGTTATGAACAGCCGTCTCGAGCTCAACAATGacacc                                                                                    >  2:626439‑M2/83‑147 (MQ=255)
                                                                  tactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAAc                                                                                    >  1:108461‑M2/83‑149 (MQ=255)
                                                                         ctgtctgttggcaacaccgtactggttgaggatggtctaatcggtatggacgttaccgccattgaaggtaacaaaCATGGCGACCAGCTGCGAACGTACCGACTGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGCAAAc                                                                             >  1:1225159‑M2/76‑149 (MQ=255)
                                                                         ctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATg                                                                                                                                                   >  2:1207774‑M2/76‑79 (MQ=255)
                                                                         ctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATg                                                                                                                                                   <  1:1207774‑M2/4‑1 (MQ=255)
                                                                              tgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACAGCGTGATGAACAGCCGTCTGGGGTTCAACGATGACAACCGTCCActgccc                                                                        >  1:1415650‑M2/71‑147 (MQ=255)
                                                                                ttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCAt                                                                      >  1:513261‑M2/69‑149 (MQ=255)
                                                                                   gcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGTGTTTTACAATGACAAACGTATAGTGCGCGTTAc                                                                   >  2:412619‑M2/66‑149 (MQ=255)
                                                                                     aacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTa                                                                                                                                >  1:1401405‑M2/64‑86 (MQ=255)
                                                                                     aacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTa                                                                                                                                <  2:1401405‑M2/23‑1 (MQ=255)
                                                                                        accgtactggttgacgatggtctgatcggtatgtaaattaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACAGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGATAATCACGCATTACCgaaa                                                              >  1:350427‑M2/61‑148 (MQ=255)
                                                                                           gtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAACCTGCGCATTACCGAAGCgg                                                           <  1:578016‑M2/92‑1 (MQ=255)
                                                                                                ggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGc                                                      >  1:1172813‑M2/53‑149 (MQ=255)
                                                                                                 gttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTCTGcc                                                     >  2:743167‑M2/52‑149 (MQ=255)
                                                                                                     acgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAACCTGCGCATTACCGTAGCGGTATCCCgtgg                                                 >  2:227316‑M2/48‑149 (MQ=255)
                                                                                                     acgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgcgg                                                 >  1:907169‑M2/48‑149 (MQ=255)
                                                                                                       gatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACTGCGGTCTCGAGGTCAACAATGACAACCGTAAACTGCGCATTACCGACGCGGTATGCCgtggtg                                               >  1:701027‑M2/46‑149 (MQ=255)
                                                                                                       gatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtggtg                                               >  1:1349878‑M2/46‑149 (MQ=255)
                                                                                                         ttgtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATCCCAAAGGTTGAGTGCGCATTACCGAAGCGGTATGCCGTGGTGcc                                             <  1:1431743‑M2/106‑1 (MQ=255)
                                                                                                          ggtctgatcggtatggaagttaccgctattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCGGTCTCGAGTTCAACGATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCg                                            <  1:1274358‑M2/107‑1 (MQ=255)
                                                                                                           ggctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGt                                           <  1:968659‑M2/108‑1 (MQ=255)
                                                                                                            tctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACAGCCCATTACAGAAGCGGTATCCCGAGGTGCCGtt                                          >  2:332503‑M2/41‑149 (MQ=255)
                                                                                                             ctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTc                                                                                                >  2:982778‑M2/40‑94 (MQ=255)
                                                                                                             ctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTc                                                                                                <  1:982778‑M2/55‑1 (MQ=255)
                                                                                                              tgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGa                                        >  1:69292‑M2/39‑149 (MQ=255)
                                                                                                                  cggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCCTTACCGAAGCGGTATGCCGTGGTGCCGTTGAAAct                                    >  1:119061‑M2/35‑149 (MQ=255)
                                                                                                                    gtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCGTTACCGAAGCGGTATACCGTGGTGCCGTTGAAActgc                                  >  2:782496‑M2/33‑149 (MQ=255)
                                                                                                                    gtatggaagttaccgccattgaaggtaacaaaCATGGAGACCCTGTTCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgc                                  <  1:269344‑M2/117‑1 (MQ=255)
                                                                                                                     tacggaagttaccgctattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgct                                 <  2:216797‑M2/118‑1 (MQ=255)
                                                                                                                       tggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGa                               >  2:559772‑M2/30‑149 (MQ=255)
                                                                                                                        ggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGtgatga                                                                                                                 <  1:1305228‑M2/38‑1 (MQ=255)
                                                                                                                        ggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGtgatga                                                                                                                 >  2:1305228‑M2/29‑66 (MQ=255)
                                                                                                                                 cgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGGTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAGACTGGAt                     >  1:542598‑M2/20‑149 (MQ=255)
                                                                                                                                   ccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtggt                                                >  2:956119‑M2/18‑120 (MQ=255)
                                                                                                                                   ccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtggt                                                <  1:956119‑M2/103‑1 (MQ=255)
                                                                                                                                   ccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGCAAAACTTGATGc                   >  1:168349‑M2/18‑149 (MQ=255)
                                                                                                                                     attgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGACAACCTGGATGCTc                 >  2:653941‑M2/16‑149 (MQ=255)
                                                                                                                                         aaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACTGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTCGACACTGCTTAAAAACTGGATGCTCCGCt             >  1:1345883‑M2/12‑149 (MQ=255)
                                                                                                                                          aggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTg            >  1:967612‑M2/11‑149 (MQ=255)
                                                                                                                                            gtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGAt          >  1:369013‑M2/9‑149 (MQ=255)
                                                                                                                                             taacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTTCCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATc         <  1:1361197‑M2/142‑1 (MQ=255)
                                                                                                                                              aacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCg        >  2:1160729‑M2/7‑149 (MQ=255)
                                                                                                                                               acaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGt       <  1:595135‑M2/144‑1 (MQ=255)
                                                                                                                                               acaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTACGCATTAACGAAGCGGTATGCCGGGGTTCCGTTGAACCTGCTGAAGAAATAGATGCTCCTCTGGTCGt       >  2:271543‑M2/6‑149 (MQ=255)
                                                                                                                                                 aaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTgg     >  1:1183713‑M2/4‑149 (MQ=255)
                                                                                                                                                 aaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTgg     >  1:702921‑M2/4‑149 (MQ=255)
                                                                                                                                                    cATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTg  <  2:1164319/149‑1 (MQ=255)
                                                                                                                                                    cATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTg  <  2:1164259/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTG  >  NC_000913_3_pae_tpiA/1756530‑1756826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑