Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,679,5250GT93.9% 189.9 / NA 67A1074D (GCT→GAT) yphGDUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (36/26);  total (39/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTG  >  NC_000913_3_pae_tpiA/2679380‑2679670
                                                                                                                                                 |                                                                                                                                                 
aGGGTATTTTGTAACAACGGTTTATTCTAAGGAGTGTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCg                                                                                                                                                <  2:877222/149‑1 (MQ=255)
 gggTTTTTATTAACAACGGCTTATTCTAATGATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGGTAACAATAAAAAGCATCACGGTAGTGAATAATGCCGCTTTCGGCCAAGCCgg                                                                                                                                               >  2:568171/1‑149 (MQ=255)
  ggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGa                                                                                                                                              <  1:722171/149‑1 (MQ=255)
   gTTTTTATTAACAACGGCTTATTCTAATCATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGAAGTCAAAAGAGAGAGTAATGCCGCTTTCGGCCAAACCGGac                                                                                                                                             >  1:1123418/1‑148 (MQ=255)
        tattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATc                                                                                                                                        <  1:355121/149‑1 (MQ=255)
         attaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCCCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCg                                                                                                                                       <  1:213860/149‑1 (MQ=255)
          ttaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCGAATCCGGATTAAtgga                                                                                                                                      >  1:23488/1‑146 (MQ=255)
            cacagcGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAGTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGACAATCCGGATTAATCGCCg                                                                                                                                    >  1:1374447/2‑149 (MQ=255)
                   gCTTATTCTAATTATTTTGTGATGGGCGGCAACGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCC‑TCAATTCa                                                                                                                            >  1:62848/1‑149 (MQ=255)
                     ttATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGGTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGACCAATCCGGATTAATCGCCGTCAATTCAg                                                                                                                           >  2:198056/1‑149 (MQ=255)
                          cTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGa                                                                                                                      >  2:269448/1‑149 (MQ=255)
                          cTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGa                                                                                                                      <  1:44779/149‑1 (MQ=255)
                           tAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAg                                                                                                                     >  2:540570/1‑149 (MQ=255)
                            aaTTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGCGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGc                                                                                                                    >  1:331208/1‑149 (MQ=255)
                               tattTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                                                                                                                 <  1:624839/149‑1 (MQ=255)
                                attTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATACTGGAGTTTATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGAc                                                                                                                <  1:1353111/149‑1 (MQ=255)
                                  tttGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTc                                                                                                              <  2:806471/149‑1 (MQ=255)
                                  tttGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTc                                                                                                              >  1:893361/1‑149 (MQ=255)
                                     gtgATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata                                                                                                           <  1:678136/149‑1 (MQ=255)
                                       gatgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGa                                                                                                                                              >  1:545396/1‑112 (MQ=255)
                                       gatgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGa                                                                                                                                              <  2:545396/112‑1 (MQ=255)
                                         tgagatgCAGTGCGTGCCGCTCAAATATTACAGGTAGTAGGGTTTAGTGATCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgc                                                                                                       <  2:425954/143‑1 (MQ=255)
                                             cGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCaa                                                                                                   <  1:199457/149‑1 (MQ=255)
                                               gCAGCGCGTGCTGCTCAAATATTACAGTTAATAGGGTTTAGTGGACGCGGTTAATAGTAATAGGGTTGACGGTGGTGAATAATGCCGCTTTCGGGCAATCCGGATTATTGGCCGTCAATTCAGCGCGGGCAGACTCCTATTGCGGAAcg                                                                                                 >  2:496130/1‑148 (MQ=255)
                                                 aGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc                                                                                               >  2:713127/1‑149 (MQ=255)
                                                      gTGCCTCTCAAATATTACATTTAATAGTGTTTATTAAACGCGGTTAAAAATAAAAGTCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGa                                                                                          <  2:23488/149‑1 (MQ=255)
                                                        gCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGACCAATCCGGATTAATCGCCGTCAATTCGGCGCGAGCAGACTCATATTGCGCAACCTccctcagt                                                                                        >  2:707724/1‑144 (MQ=255)
                                                           gttCAAATATTACAGTTTATAGGGTTTAGTGAGAGCGGTTAAAAATAACAGGTATCACGGTGGAGAGTAATGCCGCTTTCGGCCAAGCCGGAGTAATCGACGTTAATTCAGCGCGAGCACACTTATATTGCGTACCCCCCCCGAGTccc                                                                                     <  2:1307993/147‑1 (MQ=255)
                                                                     tagaGTTAATAGGGTTTAGTGAATGCGGTGAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCa                                                                           <  2:819654/146‑1 (MQ=255)
                                                                        aGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCa                                                                        >  1:59289/1‑149 (MQ=255)
                                                                             aTAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGcc                                                                   >  1:578157/1‑149 (MQ=255)
                                                                                 ggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGcaacca                                                               >  2:181651/1‑149 (MQ=255)
                                                                                    ttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATCTTGCGCAACTTCCCCGAGTCCCGCGGGCGCCAGAATCGTCACCATCa                                                            <  2:858949/149‑1 (MQ=255)
                                                                                     tAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAg                                                           <  1:1015210/149‑1 (MQ=255)
                                                                                     tAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGCCAAGTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGCCCCGCGGACGCCAGCATACCCACCATccc                                                           >  2:259799/1‑147 (MQ=255)
                                                                                        tGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCCTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCCGCATCGCCACCATccacca                                                        >  2:1214277/1‑144 (MQ=255)
                                                                                          aaCGCGGTTAAAAATAAAAGGCATCACGGTGGTGCATAATGCCGCTTTCGACCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCCTCGCCACCATCAGGCAtt                                                      >  2:54597/1‑149 (MQ=255)
                                                                                          aaCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATGCGGATTAAACGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAACTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCCtt                                                      >  1:571068/1‑149 (MQ=255)
                                                                                          aaCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAtt                                                      >  1:749247/1‑149 (MQ=255)
                                                                                               ggTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCt                                                 <  1:1175169/149‑1 (MQ=255)
                                                                                                ggtAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCAGATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCtt                                                <  2:1394621/147‑1 (MQ=255)
                                                                                                cgtAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTGCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACGCAGATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCtt                                                <  1:126681/147‑1 (MQ=255)
                                                                                                 ttAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCAAATCCGGATGAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCCCCCTCGCCACCATCAGGCATTTTTCttt                                               >  2:369578/1‑149 (MQ=255)
                                                                                                      aaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtgct                                          >  2:1139481/1‑149 (MQ=255)
                                                                                                      aaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTACCCGAGTCCCACGGACGCCACCATCGCCACCATCAGCCCTTTTTCGTTCtgct                                          >  2:67532/1‑149 (MQ=255)
                                                                                                      aaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAATCAACGCGAGCAGACTCATATTGCGCAACCTCCACAAGACCACCGGACGCCAGCATCGCCACCATCAGCCATTTTTCTATAtact                                          >  2:486764/1‑149 (MQ=255)
                                                                                                       aTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtgctg                                         >  1:521650/1‑149 (MQ=255)
                                                                                                        tAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCCCCATCAGGCATTTTTCTTTAtgctgc                                        >  2:989994/1‑149 (MQ=255)
                                                                                                          aaaGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGctgctg                                      >  1:599545/1‑149 (MQ=255)
                                                                                                              gCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCtgttgt                                  >  1:304680/1‑149 (MQ=255)
                                                                                                                       tggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCa                         >  2:1314417/1‑149 (MQ=255)
                                                                                                                        ggtgAATAGTGCCGTTTTCGGCCAAGTCGGATTAATCGCCGTAAATTCAGCGCGAGCAGACTCATATTGCGCAACTTCCCCGTGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCAt                        <  2:953812/149‑1 (MQ=255)
                                                                                                                         gtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATa                       >  1:1255379/1‑149 (MQ=255)
                                                                                                                         gtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATa                       >  1:444514/1‑149 (MQ=255)
                                                                                                                          tgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATAc                      >  2:563903/1‑149 (MQ=255)
                                                                                                                           gAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACa                     <  2:521650/149‑1 (MQ=255)
                                                                                                                           gAATAATGCCGCTTTCGGCCAATCCGGAGTAATCGCCGTCAATTCAGCGCGAGCGAACTCGTATTTCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGGTGTTGTAAATGGCTATACa                     >  2:5341/1‑149 (MQ=255)
                                                                                                                                atGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACAATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGac                <  1:1164961/149‑1 (MQ=255)
                                                                                                                                 tGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCcaca               >  2:162611/1‑149 (MQ=255)
                                                                                                                                  gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCCCCATCACCCTTGTTTCTGTATGCTGCTGTTGTAACTCGCCATACAGCGacaa              >  1:837272/1‑149 (MQ=255)
                                                                                                                                     gCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa           <  1:540570/149‑1 (MQ=255)
                                                                                                                                     gCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa           <  1:1422704/149‑1 (MQ=255)
                                                                                                                                          cGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAg      <  2:578157/149‑1 (MQ=255)
                                                                                                                                           ggCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACACCAAGTCAgg     >  1:502272/1‑149 (MQ=255)
                                                                                                                                           ggCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAgg     <  1:1314417/149‑1 (MQ=255)
                                                                                                                                             ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGtt   <  2:599545/149‑1 (MQ=255)
                                                                                                                                              cAAGCCGGGTTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCAATACAGCGACAACAAGTCAGGtcg  >  2:1075569/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTG  >  NC_000913_3_pae_tpiA/2679380‑2679670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑