Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,227,550 G→C intergenic (+123/‑97) metH → / → yjbB homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,227,5500GC89.0% 454.6 / 41.5 146intergenic (+123/‑97)metH/yjbBhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand):  ref base G (10/6);  new base C (70/59);  total (80/66)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 146 total aligned reads displayed.

TCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGG  >  NC_000913_3_pae_tpiA/4227406‑4227695
                                                                                                                                                |                                                                                                                                                 
tCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCaaa                                                                                                                                              >  4:561356/1‑150 (MQ=255)
       tATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcaca                                                                                                                                       >  3:257070/1‑150 (MQ=255)
           aCGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTaa                                                                                                                                   <  1:911105/150‑1 (MQ=255)
             gcgGACTGATTCACAAATCCGTCACGATTCCTTACAACAAACAGGGCGCTCAATGTGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAAACTTAGCCGGAAAAACGCATCTCAAAAGGCACAGTAAAg                                                                                                                                 >  2:1164003/1‑150 (MQ=255)
                 aCTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGc                                                                                                                             <  3:433043/150‑1 (MQ=255)
                       tCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAAAAATCTTACCCGGAAAAACGAACCTCAAAAGGCACAGTAAAGATGCCAGTCa                                                                                                                       >  1:1158282/1‑150 (MQ=255)
                         acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGc                                                                                                                     >  4:408526/1‑150 (MQ=255)
                         acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGc                                                                                                                     >  3:674284/1‑150 (MQ=255)
                           aaaTCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCgt                                                                                                                   <  1:1266076/150‑1 (MQ=255)
                           aaaTCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCgt                                                                                                                   >  1:1163905/1‑150 (MQ=255)
                               cTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAATGAATGTCAAAAGGAACAGTAAAGATGCCAGTCAGCGTGttt                                                                                                               >  1:64507/1‑150 (MQ=255)
                               cTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGttt                                                                                                               >  1:1016267/1‑150 (MQ=255)
                                    aCTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGt                                                                                                          >  1:569920/1‑150 (MQ=255)
                                        ttccttccAACAAACAGGGGGCTAAATGAGTGTCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGATAGAAAAATATTAGAAAGAACAACGCATCCCAACTGGCTCAGCATAGATTTCAGTCAGCGTGTTTCCGGCCCtt                                                                                                      <  2:1230803/150‑1 (MQ=255)
                                        tccattACAACAAAAAGAGAGAACAATGAGTGACCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATCATATTAGCCGGAAAATCGAATCTCAACAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCtt                                                                                                      <  2:1230750/146‑1 (MQ=255)
                                         tCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCttt                                                                                                     <  2:1016267/150‑1 (MQ=255)
                                          ccTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTg                                                                                                    >  4:796680/1‑150 (MQ=255)
                                              acaacaAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCa                                                                                                >  2:765400/1‑150 (MQ=255)
                                              acaacaAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTCGGTCa                                                                                                <  4:746162/150‑1 (MQ=255)
                                                aacaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTGGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAtt                                                                                              >  1:1429636/1‑150 (MQ=255)
                                                aacaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAtt                                                                                              <  1:1285312/150‑1 (MQ=255)
                                                 acaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTc                                                                                             >  2:112551/1‑150 (MQ=255)
                                                  caaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCgt                                                                                                                   <  1:755443/127‑1 (MQ=255)
                                                    gacaGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAAt                                                                                          >  3:195504/2‑150 (MQ=255)
                                                     acaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAAtt                                                                                         >  1:1209631/1‑150 (MQ=255)
                                                     acaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAAt                                                                                          <  1:1277723/149‑1 (MQ=255)
                                                       aGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGTAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc                                                                                       >  1:349870/1‑150 (MQ=255)
                                                         ggCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt                                                                                     >  1:942787/1‑150 (MQ=255)
                                                         ggCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt                                                                                     >  2:513681/1‑150 (MQ=255)
                                                          gcggggaaTGAGTGGCTGTTCTCTTTATTAGTATGAAACACTGATTTTGGAGACAGGCTGGAATAATCTTGGCCGGAAAAACGAGTCTCAAAGTGCACTGTTAGGAGGCCAGTCAGGGTTTTTCCGGTCCTTTTGTCATTCAATTGCTTc                                                                                    <  2:1450185/144‑1 (MQ=255)
                                                          gcgcTCAATGAGTGTCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTc                                                                                    <  1:161793/150‑1 (MQ=255)
                                                           cgcTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCa                                                                                   <  2:211243/150‑1 (MQ=255)
                                                            gcTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGAt                                                                                                                               <  1:594756/105‑1 (MQ=255)
                                                               cAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGaa                                                                               <  2:1433870/150‑1 (MQ=255)
                                                                aaTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAAc                                                                              <  1:1310123/150‑1 (MQ=255)
                                                                  tGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACat                                                                            <  1:10359/150‑1 (MQ=255)
                                                                   gcgTGCCATGTCTCTTTCTTAATATGAAACCATTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTAAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACcta                                                                           <  2:721800/148‑1 (MQ=255)
                                                                   gAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAATGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACata                                                                           <  3:754916/150‑1 (MQ=255)
                                                                     gtgtCCTGTCTCTTGATCAATATGAACCACTTCTCATGGACCTAGGCTGGAATCATCTTAGACGGAAAAAAGAATACCACCAAGCACAGTAAAAATGCCAGTCAGCGTGTTTTCGGTCCTGTGGTCATTCAATTGCTTCAGGAACAtata                                                                         <  3:86599/150‑1 (MQ=255)
                                                                      tGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATaa                                                                        <  1:173316/150‑1 (MQ=255)
                                                                        cccTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTaa                                                                                                                                   <  2:1311609/89‑1 (MQ=255)
                                                                        cccTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTaa                                                                                                                                   >  1:1311609/1‑89 (MQ=255)
                                                                        cccTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACg                                                                      >  2:1172137/1‑150 (MQ=255)
                                                                         ccTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGa                                                                     <  2:151530/150‑1 (MQ=255)
                                                                         ccTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGa                                                                     >  4:153504/1‑150 (MQ=255)
                                                                           tGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATa                                                                   >  1:432298/1‑150 (MQ=255)
                                                                            gTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATaa                                                                  <  3:865794/150‑1 (MQ=255)
                                                                             tctcTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAg                                                                 >  1:422997/1‑150 (MQ=255)
                                                                              ctctTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAgg                                                                <  3:153504/150‑1 (MQ=255)
                                                                                   tattaATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAAc                                                           <  4:674284/150‑1 (MQ=255)
                                                                                   tattaATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAAc                                                           >  1:1082978/1‑150 (MQ=255)
                                                                                    attaATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGc                                                                                                                     >  3:371149/1‑91 (MQ=255)
                                                                                    attaATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAAcc                                                          >  1:732971/1‑150 (MQ=255)
                                                                                    attaATATGAAACACTTATACTGGAAACAGGCTGGAATAAACTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAAcc                                                          >  2:725617/1‑150 (MQ=255)
                                                                                    attaAAATGAAACACTCATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGAGTTTCCGGTCCTTTGGTCATCCAATTGCTTCAGGAACATATAACGATAAGGAGAAcc                                                          >  2:1205962/1‑150 (MQ=255)
                                                                                     ttaATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACAtata                                                                         >  1:1226761/1‑134 (MQ=255)
                                                                                      taATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTg                                                        <  3:509951/150‑1 (MQ=255)
                                                                                        atatGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGaa                                                      <  2:49355/150‑1 (MQ=255)
                                                                                        atatGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGaa                                                      <  2:182660/150‑1 (MQ=255)
                                                                                           tGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAg                                                                                  >  1:1431768/1‑119 (MQ=255)
                                                                                           tGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAg                                                                                  <  2:1431768/119‑1 (MQ=255)
                                                                                           tGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTc                                                   >  2:433005/1‑150 (MQ=255)
                                                                                            gAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCg                                                  >  4:414705/1‑150 (MQ=255)
                                                                                             aaaCACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCgt                                                 <  3:796680/150‑1 (MQ=255)
                                                                                                  cTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTaa                                            <  2:381948/150‑1 (MQ=255)
                                                                                                   ttATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAGCATATAACGATAAGGAGAACCTGAACTCGTGTTAAc                                           <  4:722361/150‑1 (MQ=255)
                                                                                                   ttATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAAc                                           <  2:1213397/150‑1 (MQ=255)
                                                                                                    tataCTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACg                                          <  1:112551/150‑1 (MQ=255)
                                                                                                     ataCTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACgc                                         >  1:453963/1‑150 (MQ=255)
                                                                                                     ataCTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACgc                                         <  2:872101/150‑1 (MQ=255)
                                                                                                      taCTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACgct                                        >  1:903793/1‑150 (MQ=255)
                                                                                                       aCTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACgctg                                       >  2:1329854/1‑150 (MQ=255)
                                                                                                        cTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACgctgc                                      <  1:1025396/150‑1 (MQ=255)
                                                                                                         tGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAg                                                                                  <  4:634946/105‑1 (MQ=255)
                                                                                                         tGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACgctgct                                     >  4:871682/1‑150 (MQ=255)
                                                                                                         tGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACgctgct                                     >  2:554538/1‑150 (MQ=255)
                                                                                                         tGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACgctgct                                     >  2:248620/1‑150 (MQ=255)
                                                                                                           gAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAAcc                                                          >  2:682350/1‑127 (MQ=255)
                                                                                                           gAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAAcc                                                          <  1:682350/127‑1 (MQ=255)
                                                                                                             aaCAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCAc                                 <  4:584005/150‑1 (MQ=255)
                                                                                                              aCAGGCTGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCAcc                                <  4:297292/150‑1 (MQ=255)
                                                                                                                    tGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCttt                          >  1:378488/1‑150 (MQ=255)
                                                                                                                    tGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCttt                          >  1:378548/1‑150 (MQ=255)
                                                                                                                    tGGAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCttt                          >  2:189128/1‑150 (MQ=255)
                                                                                                                      gAATAATCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCt                        >  3:614095/1‑150 (MQ=255)
                                                                                                                          aatCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACgctgc                                      >  2:713903/1‑132 (MQ=255)
                                                                                                                          aatCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACgctgc                                      <  1:713903/132‑1 (MQ=255)
                                                                                                                           atCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGt                   >  2:168079/1‑150 (MQ=255)
                                                                                                                           atCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGt                   >  1:522471/1‑150 (MQ=255)
                                                                                                                            tCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCgt                                                 >  1:497163/1‑119 (MQ=255)
                                                                                                                            tCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCgt                                                 <  2:497534/119‑1 (MQ=255)
                                                                                                                            tCTTAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTc                  >  3:68988/1‑150 (MQ=255)
                                                                                                                              ttAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCttt                                                                                                     <  2:481641/65‑1 (MQ=255)
                                                                                                                              ttAGCCGGAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCttt                                                                                                     >  1:481641/1‑65 (MQ=255)
                                                                                                                                     gAAAAACGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGACCATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCctgctg         >  1:35703/1‑150 (MQ=255)
                                                                                                                                        aaaCGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTc                                                   >  4:244098/1‑105 (MQ=255)
                                                                                                                                        aaaCGAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTc                                                   <  3:244098/105‑1 (MQ=255)
                                                                                                                                            gAATGTCAAAAGGCGCAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAGGGAGATCGTGAACGCGTGTTAACGGTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTggg  <  2:64507/150‑1 (MQ=255)
                                                                                                                                            gAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGGACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTggg  <  2:64564/150‑1 (MQ=255)
                                                                                                                                            gAATCTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTggg  <  3:620175/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGG  >  NC_000913_3_pae_tpiA/4227406‑4227695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: