Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 1,544,022 1 bp→AA coding (39/1389 nt) narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,544,0211.A78.6% 298.9 / 80.2 103coding (40/1389 nt)narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base . (11/11);  new base A (38/43);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 8.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_000913_3_pae_tpiA1,544,0220CA95.2% 350.7 / 2.8 103L13F (TTG→TTT) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (47/51);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TTTATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑C‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAA  >  NC_000913_3_pae_tpiA/1543875‑1544170
                                                                                                                                                      |||                                                                                                                                                   
tttATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑a‑aa                                                                                                                                                    >  1:593916/1‑150 (MQ=255)
  tATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑A‑aaaa                                                                                                                                                  >  1:905351/1‑150 (MQ=255)
   aTTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑A‑aaaaa                                                                                                                                                 <  2:735210/150‑2 (MQ=255)
     tGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑A‑aaaaaga                                                                                                                                               >  1:194882/1‑147 (MQ=255)
      gagaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑A‑aaaaagat                                                                                                                                              <  2:682328/150‑5 (MQ=255)
         aTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAAc                                                                                                                                            >  1:861399/1‑150 (MQ=255)
              cGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATAACTTCGAGAAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACtt                                                                                                                                       >  3:421649/1‑149 (MQ=255)
                 tAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTAttt                                                                                                                                    >  2:1012406/1‑150 (MQ=255)
                 tAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTAttt                                                                                                                                    <  1:170394/150‑1 (MQ=255)
                   aCTGCGGTAAATAGCATACAGAGACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTACAGAAG‑A‑GAA‑AAAAGATAACGACTAttttt                                                                                                                                  <  2:219610/150‑1 (MQ=255)
                    cTGCGCTAAATAGCATCCCGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATCTCTTCCACCACGCCGCTTTCCTCTCTTTTCCCACAATGCCCGATTTTCTGGTTTCCAGTCG‑C‑G‑C‑CAAAGATAACGACTAGTTctct                                                                                                                                >  2:932372/1‑150 (MQ=255)
                        gcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCAt                                                                                                                             <  3:709114/150‑1 (MQ=255)
                        gcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCAt                                                                                                                             >  1:717021/1‑150 (MQ=255)
                          tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCAttt                                                                                                                           <  3:449370/150‑1 (MQ=255)
                            aaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTg                                                                                                                         <  1:1187654/150‑1 (MQ=255)
                            aaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTg                                                                                                                         <  3:54413/150‑1 (MQ=255)
                            aaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTg                                                                                                                         <  1:746816/150‑1 (MQ=255)
                            aaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑C‑AAAAGATAACGACTATTTTTCTCATTTTGc                                                                                                                        >  1:452181/1‑150 (MQ=255)
                             aTAGCATAAAGACAAAGAAGGCAATAATTAGATAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTAAAAAAAA‑A‑AAA‑AAAAGATAACGACTATTTTTCTCATTTTGc                                                                                                                        <  2:905351/150‑1 (MQ=255)
                                gCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑C‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTg                                                                                                                    <  2:452181/150‑1 (MQ=255)
                                 cATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTg                                                                                                                    >  2:673390/1‑150 (MQ=255)
                                 cATCCAGACACAGAAGGCAAGAAGTAGACAACGGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCA‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTg                                                                                                                    <  4:553160/150‑1 (MQ=255)
                                  aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGc                                                                                                                   >  4:17560/1‑150 (MQ=255)
                                  aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATGTCTTCGAGCAATATGTTTTCCTTTTTTTTCCCAGAAGGCCGGATTTTCTGGTTTCGCGTCG‑CTA‑A‑AAAAGATAACGACTATTTTTTTCATTGTGCAGTGc                                                                                                                   >  1:298661/1‑150 (MQ=255)
                                   tCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGcc                                                                                                                  >  1:222398/1‑150 (MQ=255)
                                     cAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑AAAAAAGATAACGACTATTTTTCTCAtttt                                                                                                                          >  4:728427/1‑140 (MQ=255)
                                     cAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑AAAAAAGATAACGACTATTTTTCTCAtttt                                                                                                                          <  3:728427/140‑1 (MQ=255)
                                     cAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTCATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCat                                                                                                                >  2:653382/1‑150 (MQ=255)
                                      aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCata                                                                                                               >  3:39593/1‑150 (MQ=255)
                                          acaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                           <  3:288226/150‑1 (MQ=255)
                                           caGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                                          <  1:369695/150‑1 (MQ=255)
                                           caGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑C‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                         <  4:635389/150‑1 (MQ=255)
                                            aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                         >  1:649013/1‑150 (MQ=255)
                                               aGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTc                                                                                                      <  3:238066/150‑1 (MQ=255)
                                                ggCAAGAAGTAGACAACTGACTGATATCGAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTAAAAAAAA‑A‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                                     <  2:559395/150‑1 (MQ=255)
                                                 gCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcac                                                                                                    >  1:565655/1‑150 (MQ=255)
                                                 gCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcac                                                                                                    >  2:473971/1‑150 (MQ=255)
                                                  cAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                                   <  2:1042354/150‑1 (MQ=255)
                                                   aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAt                                                                                                  >  1:768281/1‑150 (MQ=255)
                                                     gaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGc                                                                                                >  2:1094711/1‑150 (MQ=255)
                                                       ggTAGACAACTGAATGATCTCAAGCGCTTACTCCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAATCGAA‑A‑A‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcac                                                                                              <  4:452386/149‑1 (MQ=255)
                                                           gACAACTGACTGATATCCAGAGATTTCTTCGGGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTGCCTCACATGCACACAt                                                                                          <  2:593916/150‑1 (MQ=255)
                                                           gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGca                                                                                               >  4:639775/1‑145 (MQ=255)
                                                           gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGca                                                                                               <  3:639775/145‑1 (MQ=255)
                                                             cAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg                                                                                        <  2:778402/150‑1 (MQ=255)
                                                              aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑A‑aaaaa                                                                                                                                                 >  3:33355/1‑90 (MQ=255)
                                                                ctgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTa                                                                                     >  3:186571/1‑150 (MQ=255)
                                                                 tgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTaa                                                                                    <  2:906590/150‑1 (MQ=255)
                                                                   actgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcacaca                                                                                           >  2:809473/1‑141 (MQ=255)
                                                                       atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaa                                                                              >  3:96170/1‑150 (MQ=255)
                                                                        tatCCAGCGATTTCTTCGAGCAATATGTTTTCCTTTATTTGCCTATTAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaa                                                                             <  1:653278/150‑1 (MQ=255)
                                                                           ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                                          >  4:456347/1‑150 (MQ=255)
                                                                           ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                                          >  1:798601/1‑150 (MQ=255)
                                                                            cAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCAttt                                                                                                                           <  3:279837/100‑1 (MQ=255)
                                                                            cAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCAttt                                                                                                                           >  4:279837/1‑100 (MQ=255)
                                                                              gagaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAAc                                                                                                                                            <  1:1051930/81‑1 (MQ=255)
                                                                              gagaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑AAAAAAGATAAc                                                                                                                                            >  2:1051930/1‑81 (MQ=255)
                                                                               agaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaa                                                                      >  1:289113/1‑150 (MQ=255)
                                                                               agaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaa                                                                      >  4:184364/1‑150 (MQ=255)
                                                                                    tcttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac                                                                 <  2:1111857/150‑1 (MQ=255)
                                                                                    tcttcGAGCAATATGTTTTCCTTTATCTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac                                                                 >  2:1194917/1‑150 (MQ=255)
                                                                                    tcttcGAGCAATATGTTTTCCTTTATCTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAGTGAAAAAAAGACAAAacac                                                                 >  2:1194932/1‑150 (MQ=255)
                                                                                     cttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACg                                                                <  1:319032/150‑1 (MQ=255)
                                                                                     cttcGAATAATATTTTTTTCTTTATTTTAACAGAAGAACGAATTTTCTGGTTTCCAGTCG‑C‑G‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCAATATTGTTCCTCACATGCACATAAAGAAAAAAAAAAAAAGACAAAACACg                                                                <  2:659433/150‑1 (MQ=255)
                                                                                      ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                               >  1:892666/1‑150 (MQ=255)
                                                                                      ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                               <  3:51922/150‑1 (MQ=255)
                                                                                               tatGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgcg                                                      <  4:443469/150‑1 (MQ=255)
                                                                                                atGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgcgc                                                     <  4:231627/150‑1 (MQ=255)
                                                                                                    tttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa                                                                           <  1:1203052/124‑1 (MQ=255)
                                                                                                      tCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGc                                               >  4:548561/1‑150 (MQ=255)
                                                                                                       ccTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGcc                                              <  3:17560/150‑1 (MQ=255)
                                                                                                        cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTAG‑C‑GAA‑AAAAGATAACAACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATCCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                             <  2:117178/150‑1 (MQ=255)
                                                                                                         tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAg                                            >  1:528598/1‑150 (MQ=255)
                                                                                                         tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATAGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAg                                            >  1:676117/1‑150 (MQ=255)
                                                                                                          ttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGt                                           >  2:551069/1‑150 (MQ=255)
                                                                                                          ttATTTTCCCAGAAGGCCGGATTTACTGGTTTCAAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTGCCTCACATGCACACAATAGTAAAGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGt                                           <  2:389912/150‑1 (MQ=255)
                                                                                                           tATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGcc                                              <  4:480060/146‑1 (MQ=255)
                                                                                                           tATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGcc                                              >  3:480060/1‑146 (MQ=255)
                                                                                                            aTTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGtta                                         >  4:264678/1‑150 (MQ=255)
                                                                                                             ttttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttat                                        <  2:717021/150‑1 (MQ=255)
                                                                                                              tttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt                                       <  1:244568/150‑1 (MQ=255)
                                                                                                              tttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt                                       <  3:264678/150‑1 (MQ=255)
                                                                                                                 cccAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGa                                    <  2:1049945/150‑1 (MQ=255)
                                                                                                                    aGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGtta                                         <  3:389373/142‑1 (MQ=255)
                                                                                                                    aGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGtta                                         >  4:389373/1‑142 (MQ=255)
                                                                                                                    aGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAAtt                                 >  1:605348/1‑150 (MQ=255)
                                                                                                                         gCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAgac                            >  2:341650/1‑148 (MQ=255)
                                                                                                                                 tgcTGGTTTTCAGTCG‑A‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACAAAAAAAAAAAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTgg                    <  2:1032789/148‑1 (MQ=255)
                                                                                                                                  tCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                         >  3:347075/1‑64 (MQ=255)
                                                                                                                                  tCTGGTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                         <  4:347075/64‑1 (MQ=255)
                                                                                                                                   cTGGTTTCCAGTCG‑C‑G‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGt                  <  1:405640/150‑1 (MQ=255)
                                                                                                                                     ggTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGa                <  2:266874/150‑1 (MQ=255)
                                                                                                                                      gTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGaa               <  1:1012406/150‑1 (MQ=255)
                                                                                                                                      gTTTCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGaa               <  2:861399/150‑1 (MQ=255)
                                                                                                                                         tCCAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTg            >  3:265780/1‑150 (MQ=255)
                                                                                                                                          ccAGTCG‑C‑GAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGc           <  2:222398/150‑1 (MQ=255)
                                                                                                                                            aGTCG‑C‑G‑C‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGa        <  2:336191/150‑1 (MQ=255)
                                                                                                                                                 tcgcgaaaaaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGAt       <  2:565655/143‑1 (MQ=255)
                                                                                                                                                    g‑c‑AAAAGATAACGACTATTTTGCTCATTGTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGGTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa  <  4:577593/150‑1 (MQ=255)
                                                                                                                                                      aaaaaaGATAACGACTATTTTTCTCATTTTGTAGTTCCATATTGTTCCTCACATACACACATTGGCAATGAAAAAAAGACAAAACCCGAGGTAAGGCGCAATAGCCAGTTATCAGAATGAAGGATGAAAGGGGTGAACTGCGGATAGaaa  >  2:838988/3‑150 (MQ=255)
                                                                                                                                                      |||                                                                                                                                                   
TTTATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑C‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAA  >  NC_000913_3_pae_tpiA/1543875‑1544170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑