Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_vch_tpiA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_tpiA1,196,2450TC100.0% 29.9 / NA 13L375M (TTA→CTG) icdisocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/8);  total (5/8)

ACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGG  >  NC_000913_3_vch_tpiA/1196102‑1196314
                                                                                                                                               |                                                                     
aCATCGGTGACGAATGGGCCCTGTTTGAAGGCACGCACGGTACTGCGCCGAAATATGCAGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAtt                                                                  <  2:395268/149‑1 (MQ=38)
  aTCAGTAACGAATAACCCCTGTTTGAAGCCACCCACGGTACTGCGCCAAAATAAGCCGCTCAGGACAAAGTAAATCCAGGCTCTATTATTCCCTCCGCTGAGATGATGCTGCGCCATATGGGTTAGACTGAAGCGGCTGACCTGATTGt                                                                <  2:10302/149‑1 (MQ=25)
     ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCACCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaa                                                             >  2:34650/1‑149 (MQ=38)
      gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                            >  1:374739/1‑149 (MQ=255)
      gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                            <  2:217537/149‑1 (MQ=255)
                   ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                               >  1:369807/1‑149 (MQ=255)
                           aaGCCAACCACGGTAATGCGCCGAAATATGCCGGCCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                       <  2:196012/149‑2 (MQ=25)
                             gCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATTATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTGAAAGGTATGGAAGGCGCAATCAAtgc                                     <  1:34650/149‑1 (MQ=25)
                                acccacGGTACTGCGCCGAAATATGCCGGTCACGACAAAGTTAATCCTGGCTGTATTATGCGCTCCGTTGAGATGATGGTGCGCCAAATGGTTTGGACTGAAGCGGCTGACCTGATTCTTAAAGGTATGGAAGGCGACATCAATGCCaa                                  <  2:297459/149‑1 (MQ=18)
                                                         ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa                                                    >  1:434246/1‑106 (MQ=21)
                                                         ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa                                                    <  2:434246/106‑1 (MQ=21)
                                                             tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga     <  2:374739/149‑1 (MQ=14)
                                                                ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAGCGTCTgatgg  >  1:117475/1‑149 (MQ=14)
                                                                                                                                               |                                                                     
ACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGG  >  NC_000913_3_vch_tpiA/1196102‑1196314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: