Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	2,214,152	A→T	Y165N (TAT→AAT)	yehU ←	inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	2,214,152	0	A	T	97.9%	133.4 / NA	49	Y165N (TAT→AAT)	yehU	inner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (24/23);  minor base G (1/0);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTC  >  NC_000913_3_vch_tpiA/2214015‑2214296                                                                                                                                          |                                                                                                                                                 ggCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCGGACGCACGCGATCTTCATTAGTTGGGGCGa                                                                                                                                       >  1:97402/1‑149 (MQ=255)   cAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATc                                                                                                                                     <  1:136679/149‑1 (MQ=255)     gAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGATCGGGCGATCGc                                                                                                                                   >  1:232070/1‑149 (MQ=255)      aaaaaGCCGAAGTGTATATTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGAGGGTATTGGTGCCCATCATCTCCTCTACATTATTCCGCAACAAGCGCACCCCATCTCAATTTAGTCAGGCGATCGCa                                                                                                                                  >  2:464944/1‑149 (MQ=255)        aaaGACGAAGTTTATTTTTCAAACAGCGCGGGTTTAGCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAg                                                                                                                                <  2:97402/149‑1 (MQ=255)         aacccGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCACAAga                                                                                                                               >  2:560744/4‑148 (MQ=255)              gAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGgatgat                                                                                                                          <  1:473738/149‑1 (MQ=255)               aaGGGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCCTAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATc                                                                                                                         <  2:407191/149‑1 (MQ=255)                agcgtATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCACCCCGCTGTATTTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCa                                                                                                                        <  1:402751/146‑1 (MQ=255)                       ttttCAAACATCGCGCATTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATCGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTg                                                                                                                 >  1:727234/1‑149 (MQ=255)                              aCATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGGAAACGACGCGCTGGAGGTCCGGCGACCGCAAGGATGATCAGCATTTGCACCAtt                                                                                                          >  1:608065/1‑149 (MQ=255)                               gttCGCGCGTTTGTCGGTCAAGATACGCATAAACAGCGGCTCGCCGACGTTATTGGTGACCAGCATTGGCGCAGCAATATGACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCAttt                                                                                                         <  2:367725/147‑1 (MQ=255)                                   gcgcgTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGc                                                                                                     >  2:289265/1‑149 (MQ=255)                                    cgcgTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCCCCATTTCcgcg                                                                                                    >  2:264196/1‑149 (MQ=255)                                      cgTTTATCGAGTAATATTCGCACACACGGCGCCGCGCCGACGGTATTGGTGACCGTAATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCCTTTGCACCATATCAGcgac                                                                                                  <  2:704963/149‑1 (MQ=255)                                          tATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAAc                                                                                              >  1:673775/1‑149 (MQ=255)                                          tATCAAGCACTATACCCATAAACAGCGCCGCGCCGATGGAATTGGCGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCTGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAAc                                                                                              <  1:155231/149‑1 (MQ=255)                                           aTCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCCTTTCAGCGACGAACg                                                                                             >  2:28669/1‑149 (MQ=255)                                              gAGCAACATACGCATAAACAGCGCCGCGCGGACTCCACGCGCTACCAGCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGAGCGCAAGGATGATCAGTACTTGCACAATTCCAGCGACGCCCGTGa                                                                                          <  2:590631/149‑1 (MQ=255)                                               aGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGTCGATCGCAAGGATGATCAGCCTTTGCCCCATTTCAGCCACGAACGTGAc                                                                                         >  1:226504/1‑149 (MQ=255)                                                                 aGCCCCGCGCCGACGGTAGTGGTGACCATCATTGGCGCAGCAATATCACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATCAGCACCATTTCAGCTGCGAGCGTGACGGCACCGGCGGTAATagg                                                                       <  2:513174/149‑1 (MQ=255)                                                                  gcgccgcgccGGCTGGAGTGGTGACCATCAGTGGCGGAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCTGGCGATAGCAAGGATGATCAGCATTTGCACCATGTCAGCGACGAACGTTACGGCACCGGCGGTAATgggg                                                                      <  2:339501/149‑1 (MQ=255)                                                                  gcgccgcgccGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATgggg                                                                      <  1:729027/149‑1 (MQ=255)                                                                       gcgccgACGGTATTGGTGACCATCATTGGCGCAGCAATATTCCTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTaaa                                                                 <  2:525141/149‑1 (MQ=255)                                                                          ccgACGGTCTTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGAc                                                              <  1:732255/149‑1 (MQ=255)                                                                            gACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACtt                                                            <  1:211296/149‑1 (MQ=255)                                                                            gACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACtt                                                            <  2:243536/149‑1 (MQ=255)                                                                               ggTATTGGTGACCATGATTGGCGCAGTAATATGACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTAt                                                         <  2:152296/149‑1 (MQ=255)                                                                               ggTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTAt                                                         >  2:125646/1‑149 (MQ=255)                                                                                 tATTAGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGAATCTACATTAGGTCGTGCGAGCGCAAAAACGATCAACAATTGCACCATTTCAGCAACGAACGAGACAGCACTCGCGCTAATGGCATTAAACACTGTATCa                                                       >  2:187928/1‑149 (MQ=255)                                                                                                    cgcAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCa                                    >  1:160717/1‑149 (MQ=255)                                                                                                       agcaATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCCGTGCGCCAGCGGCGGATCAgca                                 >  1:711523/1‑147 (MQ=255)                                                                                                         caAGATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCTCACTGATGATCAGCATTTGCACCATTTCAGCGACGACCGTAACGGCACCGGCGGAAAGGCGGGTAAAGACTTTATCAGTGCGCCCGCGGCGGACCAGGATg                               >  2:11458/1‑149 (MQ=255)                                                                                                                 cTCACCAGACGCACCGCATCTTCATTAGGGCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTAc                       <  1:518308/149‑1 (MQ=255)                                                                                                                   cACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATTAGCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCa                     <  1:154640/149‑1 (MQ=255)                                                                                                                    aCCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGAGCCATCATCTTGTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTACTGGGGGTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGAGGCTGGGTCCCAg                    >  1:406665/1‑149 (MQ=255)                                                                                                                     ccAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAgg                   >  2:126609/1‑149 (MQ=255)                                                                                                                        gACGCACCGCATCTTCATTAGGGCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCCACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCa                <  1:264196/149‑1 (MQ=255)                                                                                                                          cGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCAcc              <  2:673775/149‑1 (MQ=255)                                                                                                                           gCACCGCATCTTCATTAGCTCCGCCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGCCAATGGGGTAAAAAACTTTAACAGTCCGACCGCGCAGGATCAGGATGCTGCGCACCCGGCCAcac             >  2:28823/1‑147 (MQ=255)                                                                                                                             aCCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAg           >  1:544528/1‑149 (MQ=255)                                                                                                                             aCCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAg           <  1:430165/149‑1 (MQ=255)                                                                                                                               cGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATggg                                                                       <  2:339023/87‑1 (MQ=255)                                                                                                                               cGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATggg                                                                       >  1:339023/1‑87 (MQ=255)                                                                                                                                gCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCAAAATTTCAACGAAAAACGTGACGACACCGGCGTTAATGGGGTTAAAGACTTTATTAGTGCTCCCGCGTCGGATCCGGATGCTGTTTACCAGACAAACGAGTaa        >  2:197339/1‑149 (MQ=255)                                                                                                                                  aTCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCAATTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCCGGATGCCGTCTACCAGGCCACCGCGTacgc      >  2:513758/1‑146 (MQ=255)                                                                                                                                     ttCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCtt   >  1:777583/1‑149 (MQ=255)                                                                                                                                     ttCATCAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCtt   <  1:351505/149‑1 (MQ=255)                                                                                                                                      tCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTc  >  1:222133/1‑149 (MQ=255)                                                                                                                                          |                                                                                                                                                 GGCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTC  >  NC_000913_3_vch_tpiA/2214015‑2214296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑