| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 3,560,455 | 1 | . | G | 95.3% | 130.0 / 0.3 | 43 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (18/23); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
CGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGT > NC_000913_3_vch_tpiA/3560309‑3560596 | cgctatCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑cgc < 1:283217/146‑3 (MQ=255) aaaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGt > 2:328125/1‑149 (MQ=255) aaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGtt > 2:687239/1‑149 (MQ=255) gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCGCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTTTTAGCAGACTTGAACAACGGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTc < 1:830429/149‑1 (MQ=255) tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTcc < 1:583478/149‑1 (MQ=255) gAGCCATTGGGGATTTGCTCCGCCACGTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGc < 1:533416/149‑1 (MQ=255) gCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCa < 1:106796/149‑1 (MQ=255) aTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCt < 1:121852/149‑1 (MQ=255) ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTGCACCGGACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCCGGTTTTGCTCCGCCAGCTc > 1:827542/1‑149 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGCATAAGATTGTGCTCCGCCAGCTCAtt > 2:598784/1‑149 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGAGTGGCCTCGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGCG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCAtt > 2:362828/1‑149 (MQ=255) gtttGCTCCGTCACTTTGCGGGCGATGCGCGCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg < 2:2070/148‑1 (MQ=255) tttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAgg < 1:459033/149‑1 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATAGTGCAACGGCGTGTTAAGCGAACTGGAAGGCAGCGCCGCACCGCCCTGATG‑G‑CGCAGGATAACATTTTACACAGCCAGCTCATTCACGTcgag > 2:741107/1‑147 (MQ=255) ggCCACTGTGGGGGCGATGCGGTCTTTTTCTTGGGTCGGGGTGGCTTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc < 2:678495/148‑1 (MQ=255) cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAAGCTCTTCTCCGCCCGCTCATTCACGTCgcggc > 2:205384/1‑149 (MQ=255) cACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa < 1:179391/149‑1 (MQ=255) ttGCGGGCGTGGCGCTCGTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTCACCGAACTGGAGGGCTGCTCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt < 2:93568/149‑1 (MQ=255) gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc > 2:123548/1‑149 (MQ=255) cGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg < 1:768663/149‑1 (MQ=255) tcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc > 2:480260/1‑149 (MQ=255) gCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc < 1:480260/149‑1 (MQ=255) ccTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTGGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg < 2:402201/149‑1 (MQ=255) cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg > 1:148101/1‑149 (MQ=255) cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGCCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg > 2:326112/1‑149 (MQ=255) ttGGGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt < 1:418028/149‑1 (MQ=255) cGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCATGATG‑G‑CGCCGGATCAGGTTTGGCTGCGCCAGCTCATTGAGGTCGCGGCGAATAATCTGCGGGCTGACGGAGAAATGCTCTACCAACTCCTCGCTACGGACAACACCct > 2:787657/1‑149 (MQ=255) gCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc > 1:774252/1‑149 (MQ=255) cctatctaCCGAACTGGAAGCCAGCGCCTCACCGCCATGATG‑G‑CGCATTATCATGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct < 2:510634/142‑1 (MQ=255) gtgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctc > 2:713440/1‑148 (MQ=255) gtTAGCCGAACTGGAAGGCAGCGCCGCACCGCGATGTTG‑G‑CGCAGGATCAGTTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt < 1:595117/149‑1 (MQ=255) ttAACCGAACTGGAAGGCAGGGCCGCACCGCCAGGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt < 1:768610/149‑1 (MQ=255) aCCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc < 2:253947/149‑1 (MQ=255) aCCGAACTGGAAGGCAGCGCCGAACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc < 2:252649/149‑1 (MQ=255) ccGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCACCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc > 2:4695/1‑149 (MQ=255) ccGAACTGGAAGGCAGCGCCGCACCGCAATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCttgctgagatcgg > 1:510634/1‑142 (MQ=255) ctaaCTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCACGATCAGGTTTTGCGCCGCCAGCTCATTGATGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCa < 2:837895/147‑1 (MQ=255) cGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑GAG‑CAGGATAAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACAAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAGCa > 1:338528/1‑149 (MQ=255) cTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc < 2:550213/149‑1 (MQ=255) tGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTCACATAACCCTGCTGTTTAACCAGTTCg > 2:714815/1‑149 (MQ=255) gAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat < 2:275936/149‑1 (MQ=255) gAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat < 2:190944/149‑1 (MQ=255) gCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGGCATAACCCTGCTGTTTACCCAGTTCGataata > 1:115052/1‑149 (MQ=255) tgatg‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGAAGCGAGTAATCGGCGGGCTGACGGAGAAATGCTCTCCCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACAGTCGTGAGTTTgtgt > 1:795816/1‑149 (MQ=255) | CGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGT > NC_000913_3_vch_tpiA/3560309‑3560596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |