Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	2,214,152	A→T	Y165N (TAT→AAT)	yehU ←	inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	2,214,152	0	A	T	100.0%	82.6 / NA	30	Y165N (TAT→AAT)	yehU	inner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/17);  total (13/17)

GCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTT  >  NC_000913_3_vch_tpiA/2214016‑2214295                                                                                                                                         |                                                                                                                                                gCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCAGTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGAt                                                                                                                                     >  2:509712/1‑149 (MQ=255)  cAGAAAAAGCCGAATTGTATTTTTCAAACATCGCGGGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATc                                                                                                                                    <  2:364373/149‑1 (MQ=255)  cAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTCCCTTAGGTCGGGCGATc                                                                                                                                    >  2:620015/1‑149 (MQ=255)   aGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTCCATTAGGTCGGGCGATCg                                                                                                                                   >  2:35875/1‑149 (MQ=255)                   tATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCAt                                                                                                                   >  1:237770/1‑149 (MQ=255)                            aaCCTCGCGCGTTTATCGAGCAATATACGCATACACAGCGCCGCGGCGTCACTACGGTTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCAt                                                                                                          <  1:190107/149‑1 (MQ=255)                                 cgggcgTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAg                                                                                                     <  2:460280/146‑1 (MQ=255)                                  gcgcgTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGc                                                                                                    <  2:237770/149‑1 (MQ=255)                                                    ataCGCATAAACAGCGCCGCGCCGACGGTCTTGGTGACCATCATTGGCGCAGCAAGAGTACTCACCAGATGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCCGCGACGAACGTGACGGCAcc                                                                                  >  2:399823/1‑149 (MQ=255)                                                         cATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCCTCTTCATTAGGTCGGGCGATCGCACGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACcggcgg                                                                             >  1:338875/1‑149 (MQ=255)                                                          aTAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGCCACcggcgct                                                                            >  2:652637/1‑147 (MQ=255)                                                              aCAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGCTCGCAATGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATg                                                                        >  2:74188/1‑149 (MQ=255)                                                                aggaccgcgccgACGGTTTTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGGCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAAAGTGACGGCACCGGCGGTAATggg                                                                      <  2:139957/145‑1 (MQ=255)                                                                aGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATggg                                                                      >  2:24917/1‑149 (MQ=255)                                                                  cgccgcgccgACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGt                                                                    <  1:391358/149‑1 (MQ=255)                                                                             cGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTa                                                         <  1:528969/149‑1 (MQ=255)                                                                                  ttGGTGACCATCATTGGCGCAGCAATATTACGCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGt                                                    <  2:187646/149‑1 (MQ=255)                                                                                            tcatTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTACTGGGGTTAAAGACTTTATCAGTGCGCCCgcgg                                          >  1:252016/1‑149 (MQ=255)                                                                                                tGGCGCGGCAAGATTAGTCACCGGACGCACCGCATCTTCAGTAGGTCGGGCGATCGGAAGGATGAGCAGCATTTGCACCATTTCAGCTACGAACGTGACGGCACCGGAGGTAATGGGGTTCAAGACTTTATCAGTGCGCCCGCGGCGGa                                      <  2:393267/149‑1 (MQ=255)                                                                                                      agcaATATTACTCACCAGACGCACCGCATGTGACTTAGGTCCCGAGCGCGCGGTGATGATTAGGATTTGCAACATTTCAGCGACGAAAGTGACGGCACTGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGa                                <  2:313665/149‑1 (MQ=255)                                                                                                           tatTACTCACCAGACGCACCGCATCTTTATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCtg                           <  1:126760/149‑1 (MQ=255)                                                                                                           tatTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACAGGCTTTATTGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCtg                           <  1:636463/149‑1 (MQ=255)                                                                                                                  cACCAAACTCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATAAGTTCTCCCGCTGCGGATCAGGATGCTGTGTACCa                    <  1:163685/149‑1 (MQ=255)                                                                                                                   tctaGACGCACCGCATCTGCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAg                   <  2:408418/146‑1 (MQ=255)                                                                                                                            aCCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGGCGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAg          >  1:511963/1‑149 (MQ=255)                                                                                                                            aCCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAg          <  1:638432/149‑1 (MQ=255)                                                                                                                            aCCGCATCTGCATTAGGTCGGGCGATCGCAAGGATGGTCAGCATTTGCACCATTTCAGCGACGAACGGGACGGCACCGGCGGTAATGAGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACAAGGcccacgcg          >  2:228337/1‑143 (MQ=255)                                                                                                                             ccGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGt         >  1:489339/1‑149 (MQ=255)                                                                                                                                 aTCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATc     <  1:193154/149‑1 (MQ=255)                                                                                                                                    ttCATTAGGTCGGGCGATCGCACGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCtt  <  2:531428/149‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                GCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTT  >  NC_000913_3_vch_tpiA/2214016‑2214295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑