Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	264,098	0	G	T	100.0%	98.1 / NA	33	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/14);  total (19/14)

CCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTT  >  NC_000913_3_vch_tpiA/263958‑264243                                                                                                                                             |                                                                                                                                                  cccAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGCTGCGCATGACAGTTCTCCACTATTGTCAGTTCAGGGTGCTGCTCATCAGGCAGGTATAGGGCCCATTGCAGTCCTGGCGGCGTTCGGCGTATACAGCTAGGACTcc                                                                                                                                           >  2:450604/1‑149 (MQ=255)  ccAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCg                                                                                                                                          <  1:145880/149‑1 (MQ=255)            tgtAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGCTCAGGGTGATGCTCATCAGGCAGGTATAGGGCGCATTGCGGTCCTGGCGGCGTTCGGCCGATACCGCTAGGAGTCCGGCGATTTCCg                                                                                                                                >  2:413744/1‑149 (MQ=255)                   tcCCGCTGCAGGCCCCTGGAAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATGAGGCTGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACATc                                                                                                                         >  2:175133/1‑149 (MQ=255)                    cccGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGGTGCTCATCAGGCAGGGATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTcc                                                                                                                        >  2:327835/1‑149 (MQ=255)                       gCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                                                                                                     <  2:294709/149‑1 (MQ=255)                          gCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCGCCACTATTGTCAGTTCAGGGTGATGCTCATGAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCg                                                                                                                  <  2:127695/149‑1 (MQ=255)                              gCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                                                                                                              >  1:195573/1‑149 (MQ=255)                                 ccTGGTAGGGATGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATGGGGGCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATGCCCGGTACGTCCCTGCCGGtatact                                                                                                           >  2:38566/1‑147 (MQ=37)                                        gggTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc                                                                                                    <  1:605326/149‑1 (MQ=255)                                             gCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAc                                                                                               <  2:195573/149‑1 (MQ=255)                                                             cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAttt                                                                               <  2:390959/149‑1 (MQ=255)                                                              aCTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTg                                                                              >  2:631955/1‑149 (MQ=255)                                                               cTATTGTGAGTTCAGGGCGATGCTCATCAGGCAGTTATAGGGCCCATGGCGGTCCGGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGc                                                                             <  2:624103/149‑1 (MQ=21)                                                                        gTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATcc                                                                                                                                 <  1:75262/88‑1 (MQ=255)                                                                        gTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATcc                                                                                                                                 >  2:75262/1‑88 (MQ=255)                                                                        gTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGt                                                                    >  2:345839/1‑149 (MQ=255)                                                                         ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAtt                                                                                >  2:62437/1‑136 (MQ=255)                                                                         ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAtt                                                                                <  1:62437/136‑1 (MQ=255)                                                                           cAGGGTGAGGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGc                                                                 <  2:566671/149‑1 (MQ=255)                                                                                             gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCCATATCCGGAACGTCCCTGCCGGTGTACTGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGGAAGATTCGGGACTTAg                                               >  2:434038/1‑149 (MQ=18)                                                                                                   ataGGGCCCATTGCGGTCCTGGCCGCGTGCGGGCTATACCGCTAGGCCTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGGAGTAGCGAAAGATTCGGGACTCAGGATGCt                                         <  2:478627/149‑1 (MQ=18)                                                                                                    taGGGCCCATTGCGGTCCTGTCGGCGTTCGGCGTATACCGCTGGGTGTGCGGCGATATCCGGAACGTGCCTGCCGGTCTACCGTCTGGCACTCCGGTTCCGCTGGTATTTTCCTGTGCCGTCCCGAATGATTCGAGCCCCCGGctgctg                                        >  2:243894/1‑149 (MQ=255)                                                                                                       ggCCCATTGCGGTCCTGGCGGCGTGTGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCg                                     <  2:395094/149‑1 (MQ=255)                                                                                                                 ggTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTc                           >  1:569515/1‑149 (MQ=255)                                                                                                                              ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGtt              >  1:603934/1‑149 (MQ=255)                                                                                                                              ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGtt              <  1:631955/149‑1 (MQ=255)                                                                                                                              ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAACGAAAGATTCGGGACTCAGGATGCTGCCGGTATATCGTCATTGCCCTGCGtt              >  2:202972/1‑149 (MQ=255)                                                                                                                                 ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGGCGGTGTAATGACAGACGCTACCGTGCCCCTGGGCTTTGCCGGTGCAGGAGCGAAAGAAATGGGGCTCAGGATGCCCGCGGTAAATCGGAATTTCACTGCGATTAc           >  2:531631/1‑149 (MQ=17)                                                                                                                                  gCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACt          >  1:170097/1‑149 (MQ=255)                                                                                                                                        aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGc                      <  2:587586/131‑1 (MQ=255)                                                                                                                                        aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGc                      >  1:587586/1‑131 (MQ=255)                                                                                                                                        aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGGGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGGGGTACATCGTCATTGCCCTACGTTTCCTGATAAt    >  2:971/1‑149 (MQ=14)                                                                                                                                          cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAAttt  >  2:90762/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                  CCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTT  >  NC_000913_3_vch_tpiA/263958‑264243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑