| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,904,917 | G→T | 7.3% | C227* (TGC→TGA) | bglF ← | beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,904,917 | 0 | G | T | 7.3% | 209.8 / 4.5 | 65 | C227* (TGC→TGA) | bglF | beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase |
| Reads supporting (aligned to +/- strand): ref base G (32/28); new base T (3/2); total (35/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATG > NC_000913/3904782‑3905051 | tccGCTTATCCTGGTTTATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAAtgctg < 7:130734/134‑1 (MQ=255) cGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGca > 1:209025/1‑136 (MQ=255) gattATCCAGGTTTATCGCGGCCCCACCAGCATAATGGTGACGGGTGTTATAACCATCAGACATAGCAATGGTGTTAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGcac < 1:109338/134‑1 (MQ=255) ggTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTATCCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAgg > 1:142162/1‑134 (MQ=255) gATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGaaa > 5:223959/1‑136 (MQ=255) gCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTTCACAACCAGGCAGAAAAaat > 5:91021/1‑136 (MQ=255) gCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTTCACAACCAGGCAGAAAAaat > 3:276674/1‑136 (MQ=255) gCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAaat > 7:91124/1‑136 (MQ=255) gCGGCCCCACCAGCAGAAAGGTGACGGGGGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAaata < 4:101101/137‑1 (MQ=255) cGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAACGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAaataa > 4:189168/1‑137 (MQ=255) cccACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATgg > 7:172707/1‑136 (MQ=255) cccACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATgg > 1:240121/1‑136 (MQ=255) ccccctgcagAATGTTGACGGGGGTTATAACAATCCGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACTGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGa < 3:24179/131‑1 (MQ=255) ccaccaGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGa < 1:60000/137‑1 (MQ=255) ccaccaGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGa < 3:267123/137‑1 (MQ=255) aGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGAc > 6:1876/1‑137 (MQ=255) ggTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACg < 1:181748/137‑1 (MQ=255) gggTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAAt > 1:77521/1‑137 (MQ=255) gggTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGAAGAAAAAATAATGGGAATAACCGATGACGAGTAAt > 2:153205/1‑137 (MQ=255) aCCATCAGACATAGCAATGGTGTTAAGAAATTTTTTATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAAtgt < 2:120359/137‑1 (MQ=255) aCCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAAtgt < 2:177401/137‑1 (MQ=255) acAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACAGTAAACAAGCATTAAATAGGAGTACCAGAATGATTAACAACAAGTCAGAAAAAATAATGATAATAACCGAAGACGAGAAATTCAACAATgagaa > 4:236633/2‑135 (MQ=255) cAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCg < 3:66226/137‑1 (MQ=255) gACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCgg > 1:144139/1‑136 (MQ=255) aTAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATa > 4:120977/1‑137 (MQ=255) aTAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAAAGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATa > 4:115582/1‑137 (MQ=255) gCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAg < 2:188719/77‑1 (MQ=255) gCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAg > 1:188719/1‑77 (MQ=255) cAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAAt > 7:264704/1‑121 (MQ=255) cAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAAt < 8:264704/121‑1 (MQ=255) cAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCg > 3:244420/1‑128 (MQ=255) cAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCg < 4:244420/128‑1 (MQ=255) cAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATAcc > 6:305726/1‑135 (MQ=255) cAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATAcc > 5:44480/1‑135 (MQ=255) cAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATAcc > 7:79424/1‑135 (MQ=255) cAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATAcc > 1:144819/1‑135 (MQ=255) cAATGGTGTGAAGAAATTTTTGATTGACGACTTTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACAAGGCAGAAAAAATAATGTGAATAACCGATGACTAGTAATTCAACAATGTGACCGGAAAAcc > 5:275494/1‑135 (MQ=255) gtgtGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGaaa < 8:91124/137‑1 (MQ=255) tgtgAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAAt > 7:190344/1‑137 (MQ=255) gtgAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATc > 3:252194/1‑137 (MQ=255) aagaaATTTTTGATTGCCGACGGTACCCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAg > 2:19872/1‑137 (MQ=255) aagaaATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAg > 8:214114/1‑137 (MQ=255) agaaATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGc < 2:77521/137‑1 (MQ=255) gaaATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGcc > 5:299666/1‑137 (MQ=255) aTTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGccc > 1:177930/1‑135 (MQ=255) ttttGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAgc > 1:8296/1‑137 (MQ=255) tttGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAgcg > 1:285406/1‑137 (MQ=255) tGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATCGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCa > 4:234921/1‑137 (MQ=255) gATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCa < 5:239773/136‑1 (MQ=255) gATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCa < 3:52235/136‑1 (MQ=255) gATTGCCGAAGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCa < 6:157384/136‑1 (MQ=255) tAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGcc < 7:121343/137‑1 (MQ=255) tAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGcc < 8:172707/137‑1 (MQ=255) cATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGcc < 8:273355/129‑1 (MQ=255) cATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGcc > 7:273355/1‑129 (MQ=255) ttAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGaa < 1:193023/137‑1 (MQ=255) ttAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGaa < 5:305726/137‑1 (MQ=255) ttAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGa > 2:299899/1‑136 (MQ=255) gTCGGCGTTCCAGAATGCTTCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCa < 6:91021/137‑1 (MQ=255) gTCGGCGTTCCAGAATGCTTCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCa < 4:276674/137‑1 (MQ=255) gTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCa < 2:142162/137‑1 (MQ=255) gTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCa < 5:208434/137‑1 (MQ=255) gTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAAAGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCa < 7:154336/137‑1 (MQ=255) aaTGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATg < 7:21354/137‑1 (MQ=255) aaTGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATg < 8:190344/137‑1 (MQ=255) aaTGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATg < 6:223959/137‑1 (MQ=255) ctgcACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATg > 5:24109/1‑137 (MQ=255) | TTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATG > NC_000913/3904782‑3905051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |