breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 22 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 707,992 | 0 | C | A | 52.6% | 4.6 / 13.0 | 21 | intergenic (+235/‑342) | glnS/chiP | glutamine‑‑tRNA ligase/chitobiose outer membrane channel |
* | NC_000913 | 3,455,691 | 0 | C | A | 52.6% | 17.9 / 27.1 | 23 | F38L (TTC→TTA) | gspC | Type II secretion system protein GspC |
* | NC_000913 | 1,879,848 | 0 | C | G | 50.0% | 25.8 / 15.1 | 33 | G34A (GGA→GCA) | yeaR | DUF1971 domain‑containing protein YeaR |
* | NC_000913 | 4,220,018 | 0 | G | T | 44.8% | 12.2 / 10.9 | 29 | D475Y (GAT→TAT) | aceK | isocitrate dehydrogenase kinase/phosphatase |
* | NC_000913 | 2,519,207 | 0 | T | A | 43.5% | 5.8 / 11.3 | 23 | intergenic (+2/+50) | yfeD/gltX | putative DNA‑binding transcriptional regulator YfeD/glutamate‑‑tRNA ligase |
* | NC_000913 | 3,455,677 | 0 | A | T | 42.9% | 12.3 / 12.9 | 28 | N34Y (AAT→TAT) | gspC | Type II secretion system protein GspC |
* | NC_000913 | 1,982,973 | 0 | G | C | 42.4% | 39.5 / 16.1 | 33 | L190V (CTG→GTG) | araH | arabinose ABC transporter membrane subunit |
* | NC_000913 | 1,982,994 | 0 | A | C | 41.9% | 16.4 / 10.9 | 31 | C183G (TGT→GGT) | araH | arabinose ABC transporter membrane subunit |
* | NC_000913 | 18,110 | 0 | T | G | 40.0% | 13.7 / 11.3 | 25 | Y208D (TAT→GAT) | nhaA | Na(+):H(+) antiporter NhaA |
* | NC_000913 | 1,879,846 | 0 | T | G | 40.0% | 19.2 / 19.6 | 35 | T35P (ACG→CCG) | yeaR | DUF1971 domain‑containing protein YeaR |
* | NC_000913 | 361,307 | 0 | A | T | 38.2% | 38.2 / 10.8 | 35 | I185K (ATA→AAA) | lacA | galactoside O‑acetyltransferase |
* | NC_000913 | 1,982,991 | 0 | G | C | 38.2% | 49.1 / 10.4 | 34 | L184V (CTG→GTG) | araH | arabinose ABC transporter membrane subunit |
* | NC_000913 | 3,454,069 | 0 | G | T | 37.9% | 19.4 / 12.3 | 29 | Q444K (CAA→AAA) | gspA | Type II secretion system protein GspA |
* | NC_000913 | 931,029 | 0 | G | C | 37.8% | 49.5 / 12.1 | 37 | intergenic (‑67/+56) | cydD/trxB | glutathione/L‑cysteine ABC exporter subunit CydD/thioredoxin reductase |
* | NC_000913 | 1,879,895 | 0 | A | T | 33.3% | 61.5 / 16.0 | 42 | N18K (AAT→AAA) | yeaR | DUF1971 domain‑containing protein YeaR |
* | NC_000913 | 4,228,079 | 0 | A | G | 32.0% | 32.8 / 10.1 | 26 | I117V (ATT→GTT) | yjbB | putative inorganic phosphate export protein |
* | NC_000913 | 2,045,316 | 0 | A | C | 31.6% | 52.7 / 10.3 | 38 | T127P (ACC→CCC) | yeeJ | inverse autotransporter adhesin |
* | NC_000913 | 3,933,222 | 0 | G | T | 30.6% | 49.3 / 10.8 | 36 | intergenic (+38/‑129) | kup/rbsD | K(+):H(+) symporter Kup/D‑ribose pyranase |
* | NC_000913 | 3,108,378 | 0 | G | T | 29.2% | 73.7 / 16.3 | 48 | Q260K (CAA→AAA) | speC | ornithine decarboxylase, biosynthetic |
* | NC_000913 | 411,596 | 0 | C | A | 27.9% | 49.8 / 11.9 | 43 | A296S (GCT→TCT) | araJ | putative transport protein AraJ |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 2559874 | 50 (1.110) | 5 (0.110) | 3/252 | 4.7 | 9.1% | coding (1017/1209 nt) | intZ | CPZ‑55 prophage; putative phage integrase IntZ |
? | NC_000913 | 2559878 = | 50 (1.120) | coding (1021/1209 nt) | intZ | CPZ‑55 prophage; putative phage integrase IntZ |