breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 50 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,177,255 | 0 | G | T | 61.0% | 12.5 / 17.1 | 41 | intergenic (‑51/+257) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 2,177,234 | 0 | C | A | 60.9% | 5.3 / 10.8 | 36 | intergenic (‑30/+278) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 454,919 | 0 | G | T | 57.1% | 2.9 / 14.7 | 24 | intergenic (+130/‑214) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
* | NC_000913 | 454,906 | 0 | G | T | 55.6% | 6.7 / 16.4 | 22 | intergenic (+117/‑227) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
* | NC_000913 | 4,220,007 | 0 | T | G | 51.3% | 8.4 / 31.1 | 39 | V471G (GTG→GGG) | aceK | isocitrate dehydrogenase kinase/phosphatase |
* | NC_000913 | 3,815,809 | 0 | G | C | 50.0% | 17.2 / 12.4 | 22 | intergenic (‑41/+54) | pyrE/rph | orotate phosphoribosyltransferase/truncated RNase PH |
* | NC_000913 | 4,378,973 | 0 | G | C | 47.6% | ‑5.9 / 12.0 | 21 | intergenic (‑29/+34) | ampC/frdD | beta‑lactamase/fumarate reductase membrane protein FrdD |
* | NC_000913 | 4,220,016 | 0 | A | T | 45.0% | 21.5 / 29.0 | 40 | Y474F (TAT→TTT) | aceK | isocitrate dehydrogenase kinase/phosphatase |
* | NC_000913 | 3,086,639 | 0 | A | G | 44.0% | 18.7 / 13.8 | 27 | D4D (GAT→GAC) | yqgD | DUF2684 domain‑containing protein YqgD |
* | NC_000913 | 2,177,239 | 0 | T | A | 43.8% | 0.5 / 11.1 | 32 | intergenic (‑35/+273) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 2,177,267 | 0 | C | A | 43.8% | 42.0 / 10.4 | 49 | intergenic (‑63/+245) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 3,955,616 | 0 | T | G | 43.8% | 25.3 / 14.2 | 32 | F96V (TTT→GTT) | ilvA | threonine deaminase |
* | NC_000913 | 2,177,261 | 0 | A | T | 42.9% | 33.0 / 25.9 | 42 | intergenic (‑57/+251) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 2,177,221 | 0 | T | G | 41.0% | 9.9 / 21.0 | 39 | intergenic (‑17/+291) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 580,761 | 0 | A | C | 40.9% | 21.7 / 11.5 | 22 | intergenic (‑316/‑73) | ylcI/nohD | DLP12 prophage; DUF3950 domain‑containing protein YlcI/DLP12 prophage; putative DNA‑packaging protein NohD |
* | NC_000913 | 4,220,018 | 0 | G | T | 40.5% | 23.1 / 15.6 | 38 | D475Y (GAT→TAT) | aceK | isocitrate dehydrogenase kinase/phosphatase |
* | NC_000913 | 2,725,829 | 0 | G | T | 40.5% | 51.1 / 29.7 | 42 | intergenic (‑83/+240) | kgtP/rrfG | alpha‑ketoglutarate:H(+) symporter/5S ribosomal RNA |
* | NC_000913 | 2,725,824 | 0 | C | A | 39.0% | 66.5 / 12.6 | 42 | intergenic (‑78/+245) | kgtP/rrfG | alpha‑ketoglutarate:H(+) symporter/5S ribosomal RNA |
* | NC_000913 | 497,610 | 0 | G | C | 38.5% | 8.3 / 10.7 | 27 | D146H (GAC→CAC) | adk | adenylate kinase |
* | NC_000913 | 984,064 | 0 | T | G | 37.5% | 40.2 / 12.3 | 48 | F139V (TTT→GTT) | gloC | hydroxyacylglutathione hydrolase GloC |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 3612454 = | 45 (0.800) | 4 (0.070) | 4/250 | 5.3 | 7.8% | coding (590/1050 nt) | yhhT | putative transporter YhhT |
? | NC_000913 | = 4354938 | 50 (0.910) | intergenic (‑221/+16) | lysU/dtpC | lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase/dipeptide/tripeptide:H(+) symporter DtpC | |||||
* | ? | NC_000913 | 2940660 = | 66 (1.180) | 4 (0.070) | 4/252 | 5.3 | 6.5% | coding (584/1101 nt) | rlmM | 23S rRNA 2'‑O‑ribose C2498 methyltransferase |
? | NC_000913 | = 3037941 | 50 (0.910) | coding (166/1734 nt) | recJ | ssDNA‑specific exonuclease RecJ | |||||
* | ? | NC_000913 | 4498181 = | NA (NA) | 4 (0.070) | 3/256 | 5.9 | NA | noncoding (1/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 4499512 | NA (NA) | intergenic (+12/+81) | insD‑6/yjgX | KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment | |||||
* | ? | NC_000913 | = 2728648 | NA (NA) | 3 (0.050) | 3/254 | 5.9 | NA | noncoding (537/2904 nt) | rrlG | 23S ribosomal RNA |
? | NC_000913 | 2728653 = | NA (NA) | noncoding (532/2904 nt) | rrlG | 23S ribosomal RNA | |||||
* | ? | NC_000913 | 381258 = | NA (NA) | 8 (0.140) | 3/254 | 5.9 | NA | pseudogene (3/417 nt) | yaiX | putative acyltransferase, N‑terminal fragment |
? | NC_000913 | = 382589 | NA (NA) | noncoding (1330/1331 nt) | IS2 | repeat region |