breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 50 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2550GT61.0% 12.5 / 17.1 41intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,177,2340CA60.9% 5.3 / 10.8 36intergenic (‑30/+278)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913454,9190GT57.1% 2.9 / 14.7 24intergenic (+130/‑214)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_000913454,9060GT55.6% 6.7 / 16.4 22intergenic (+117/‑227)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009134,220,0070TG51.3% 8.4 / 31.1 39V471G (GTG→GGG) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_0009133,815,8090GC50.0% 17.2 / 12.4 22intergenic (‑41/+54)pyrE/rphorotate phosphoribosyltransferase/truncated RNase PH
*NC_0009134,378,9730GC47.6% ‑5.9 / 12.0 21intergenic (‑29/+34)ampC/frdDbeta‑lactamase/fumarate reductase membrane protein FrdD
*NC_0009134,220,0160AT45.0% 21.5 / 29.0 40Y474F (TAT→TTT) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_0009133,086,6390AG44.0% 18.7 / 13.8 27D4D (GAT→GACyqgDDUF2684 domain‑containing protein YqgD
*NC_0009132,177,2390TA43.8% 0.5 / 11.1 32intergenic (‑35/+273)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,177,2670CA43.8% 42.0 / 10.4 49intergenic (‑63/+245)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009133,955,6160TG43.8% 25.3 / 14.2 32F96V (TTT→GTT) ilvAthreonine deaminase
*NC_0009132,177,2610AT42.9% 33.0 / 25.9 42intergenic (‑57/+251)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,177,2210TG41.0% 9.9 / 21.0 39intergenic (‑17/+291)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913580,7610AC40.9% 21.7 / 11.5 22intergenic (‑316/‑73)ylcI/nohDDLP12 prophage; DUF3950 domain‑containing protein YlcI/DLP12 prophage; putative DNA‑packaging protein NohD
*NC_0009134,220,0180GT40.5% 23.1 / 15.6 38D475Y (GAT→TAT) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_0009132,725,8290GT40.5% 51.1 / 29.7 42intergenic (‑83/+240)kgtP/rrfGalpha‑ketoglutarate:H(+) symporter/5S ribosomal RNA
*NC_0009132,725,8240CA39.0% 66.5 / 12.6 42intergenic (‑78/+245)kgtP/rrfGalpha‑ketoglutarate:H(+) symporter/5S ribosomal RNA
*NC_000913497,6100GC38.5% 8.3 / 10.7 27D146H (GAC→CAC) adkadenylate kinase
*NC_000913984,0640TG37.5% 40.2 / 12.3 48F139V (TTT→GTT) gloChydroxyacylglutathione hydrolase GloC

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3612454 =45 (0.800)4 (0.070) 4/250 5.3 7.8% coding (590/1050 nt) yhhT putative transporter YhhT
?NC_000913 = 4354938 50 (0.910)intergenic (‑221/+16) lysU/dtpC lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase/dipeptide/tripeptide:H(+) symporter DtpC
* ? NC_000913 2940660 =66 (1.180)4 (0.070) 4/252 5.3 6.5% coding (584/1101 nt) rlmM 23S rRNA 2'‑O‑ribose C2498 methyltransferase
?NC_000913 = 3037941 50 (0.910)coding (166/1734 nt) recJ ssDNA‑specific exonuclease RecJ
* ? NC_000913 4498181 =NA (NA)4 (0.070) 3/256 5.9 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD‑6/yjgX KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
* ? NC_000913 = 2728648NA (NA)3 (0.050) 3/254 5.9 NA noncoding (537/2904 nt) rrlG 23S ribosomal RNA
?NC_000913 2728653 = NA (NA)noncoding (532/2904 nt) rrlG 23S ribosomal RNA
* ? NC_000913 381258 =NA (NA)8 (0.140) 3/254 5.9 NA pseudogene (3/417 nt) yaiX putative acyltransferase, N‑terminal fragment
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region