breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 113 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913707,9760TG61.3% ‑2.5 / 16.0 31intergenic (+219/‑358)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_000913708,0040CA60.7% 2.4 / 22.2 30intergenic (+247/‑330)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009132,177,2450GT59.2% ‑5.5 / 27.5 54intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913707,9890CA55.6% 4.6 / 16.1 31intergenic (+232/‑345)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009132,177,2340CA55.0% ‑4.7 / 23.2 54intergenic (‑30/+278)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913707,9920CA53.8% 4.4 / 16.0 31intergenic (+235/‑342)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_000913454,8730CG51.6% ‑3.4 / 21.4 35intergenic (+84/‑260)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009132,470,4270CA51.5% 19.2 / 36.3 36Q433K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009131,097,1090AT51.3% 4.1 / 34.5 38intergenic (+280/+456)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_000913707,9810CG50.0% 3.8 / 12.5 32intergenic (+224/‑353)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_000913707,9840CG50.0% 10.6 / 13.8 32intergenic (+227/‑350)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009132,177,2550GT50.0% 5.2 / 36.9 63intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913454,9330TA48.6% 1.8 / 22.7 40intergenic (+144/‑200)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009132,177,2210TG48.1% 29.7 / 27.5 54intergenic (‑17/+291)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009131,097,0920TC45.5% 2.5 / 12.3 32intergenic (+263/+473)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_000913454,9340AC45.0% 22.9 / 15.5 40intergenic (+145/‑199)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009134,220,0160AT44.4% 22.0 / 24.2 36Y474F (TAT→TTT) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_000913454,9260GC44.0% 17.7 / 13.2 40intergenic (+137/‑207)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_000913454,8660GT43.8% 4.5 / 10.5 32intergenic (+77/‑267)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009131,879,8460TG42.9% 36.1 / 20.2 49T35P (ACG→CCG) yeaRDUF1971 domain‑containing protein YeaR

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4498181 =NA (NA)13 (0.160) 6/256 5.9 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD‑6/yjgX KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
* ? NC_000913 381258 =NA (NA)15 (0.180) 6/254 5.9 NA pseudogene (3/417 nt) yaiX putative acyltransferase, N‑terminal fragment
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region
* ? NC_000913 2830502 =76 (0.930)3 (0.040) 3/230 7.0 3.8% coding (690/966 nt) gutQ D‑arabinose 5‑phosphate isomerase GutQ
?NC_000913 = 3379857 85 (1.160)coding (141/1128 nt) zapE cell division factor ZapE with ATPase activity
* ? NC_000913 = 83507074 (0.900)3 (0.040)
+C
3/254 7.4 4.0% coding (2001/2151 nt) dinG ATP‑dependent DNA helicase DinG
?NC_000913 4345712 = 71 (0.870)coding (1615/1647 nt) fumB fumarase B