breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 69 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT70.2% 15.2 / 13.6 48intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009134,606,1640GC63.2% ‑1.8 / 19.1 19noncoding (2/87 nt)leuVtRNA‑Leu
*NC_0009132,470,4270CA60.9% 7.8 / 23.8 24Q433K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_000913454,9060GT59.3% 6.2 / 23.4 35intergenic (+117/‑227)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_000913454,9260GA56.0% ‑4.4 / 29.7 38intergenic (+137/‑207)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009134,606,2030TG55.6% ‑3.6 / 12.1 19noncoding (84/87 nt)leuPtRNA‑Leu
*NC_000913454,9190GT51.4% 19.6 / 38.5 38intergenic (+130/‑214)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009132,177,2210TG50.0% 18.3 / 31.2 51intergenic (‑17/+291)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,177,2550GT49.0% ‑6.5 / 31.4 52intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009134,378,9730GC47.6% ‑5.1 / 12.4 21intergenic (‑29/+34)ampC/frdDbeta‑lactamase/fumarate reductase membrane protein FrdD
*NC_0009131,879,8480CG46.0% 27.2 / 19.5 37G34A (GGA→GCA) yeaRDUF1971 domain‑containing protein YeaR
*NC_0009134,220,0070TG45.7% 7.6 / 18.2 35V471G (GTG→GGG) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_0009132,177,2280CG44.4% 16.4 / 24.7 51intergenic (‑24/+284)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913931,0290GC43.2% 40.1 / 14.2 37intergenic (‑67/+56)cydD/trxBglutathione/L‑cysteine ABC exporter subunit CydD/thioredoxin reductase
*NC_000913454,9340AC42.5% 18.0 / 15.3 40intergenic (+145/‑199)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009132,470,4050TG41.9% 25.1 / 17.0 31V425V (GTT→GTGyfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,470,4110AT41.9% 23.9 / 16.5 31L427F (TTA→TTTyfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009134,220,0160AT41.2% 31.8 / 20.9 34Y474F (TAT→TTT) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_0009133,455,6690TG41.0% 41.7 / 10.6 39I31S (ATT→AGT) gspCType II secretion system protein GspC
*NC_000913931,0280AC40.5% 29.5 / 11.2 37intergenic (‑66/+57)cydD/trxBglutathione/L‑cysteine ABC exporter subunit CydD/thioredoxin reductase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3720633 =NA (NA)5 (0.080) 5/258 5.5 6.0% noncoding (1/1443 nt) IS150 repeat region
?NC_000913 = 3721544 79 (1.270)noncoding (912/1443 nt) IS150 repeat region
* ? NC_000913 2068944 =NA (NA)10 (0.160) 4/252 5.8 NA noncoding (1328/1331 nt) IS2 repeat region
?NC_000913 = 2070275 NA (NA)intergenic (‑95/‑2) insC‑3/yoeA CP4‑44 prophage; IS2 insertion element repressor InsA/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA
* ? NC_000913 3186094 =NA (NA)10 (0.160) 4/258 5.9 NA pseudogene (70/71 nt) yqiG putative outer membrane usher protein YqiG
?NC_000913 = 3187426 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 2996360 =NA (NA)5 (0.080)
+C
4/256 5.9 NA pseudogene (1/399 nt) ygeO putative uncharacterized protein YgeO
?NC_000913 = 2997691 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 2070271NA (NA)10 (0.160) 4/258 5.9 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 2996359 = NA (NA)pseudogene (2/399 nt) ygeO putative uncharacterized protein YgeO
* ? NC_000913 2068941 =NA (NA)9 (0.140) 4/258 5.9 NA noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 2070273 NA (NA)intergenic (‑93/‑4) insC‑3/yoeA CP4‑44 prophage; IS2 insertion element repressor InsA/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA
* ? NC_000913 3406402 =63 (1.010)3 (0.050) 3/234 6.1 4.8% coding (486/1350 nt) accC biotin carboxylase
?NC_000913 = 3406398 63 (1.120)coding (482/1350 nt) accC biotin carboxylase