breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 69 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,177,245 | 0 | G | T | 70.2% | 15.2 / 13.6 | 48 | intergenic (‑41/+267) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 4,606,164 | 0 | G | C | 63.2% | ‑1.8 / 19.1 | 19 | noncoding (2/87 nt) | leuV | tRNA‑Leu |
* | NC_000913 | 2,470,427 | 0 | C | A | 60.9% | 7.8 / 23.8 | 24 | Q433K (CAA→AAA) | yfdI | CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase |
* | NC_000913 | 454,906 | 0 | G | T | 59.3% | 6.2 / 23.4 | 35 | intergenic (+117/‑227) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
* | NC_000913 | 454,926 | 0 | G | A | 56.0% | ‑4.4 / 29.7 | 38 | intergenic (+137/‑207) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
* | NC_000913 | 4,606,203 | 0 | T | G | 55.6% | ‑3.6 / 12.1 | 19 | noncoding (84/87 nt) | leuP | tRNA‑Leu |
* | NC_000913 | 454,919 | 0 | G | T | 51.4% | 19.6 / 38.5 | 38 | intergenic (+130/‑214) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
* | NC_000913 | 2,177,221 | 0 | T | G | 50.0% | 18.3 / 31.2 | 51 | intergenic (‑17/+291) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 2,177,255 | 0 | G | T | 49.0% | ‑6.5 / 31.4 | 52 | intergenic (‑51/+257) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 4,378,973 | 0 | G | C | 47.6% | ‑5.1 / 12.4 | 21 | intergenic (‑29/+34) | ampC/frdD | beta‑lactamase/fumarate reductase membrane protein FrdD |
* | NC_000913 | 1,879,848 | 0 | C | G | 46.0% | 27.2 / 19.5 | 37 | G34A (GGA→GCA) | yeaR | DUF1971 domain‑containing protein YeaR |
* | NC_000913 | 4,220,007 | 0 | T | G | 45.7% | 7.6 / 18.2 | 35 | V471G (GTG→GGG) | aceK | isocitrate dehydrogenase kinase/phosphatase |
* | NC_000913 | 2,177,228 | 0 | C | G | 44.4% | 16.4 / 24.7 | 51 | intergenic (‑24/+284) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 931,029 | 0 | G | C | 43.2% | 40.1 / 14.2 | 37 | intergenic (‑67/+56) | cydD/trxB | glutathione/L‑cysteine ABC exporter subunit CydD/thioredoxin reductase |
* | NC_000913 | 454,934 | 0 | A | C | 42.5% | 18.0 / 15.3 | 40 | intergenic (+145/‑199) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
* | NC_000913 | 2,470,405 | 0 | T | G | 41.9% | 25.1 / 17.0 | 31 | V425V (GTT→GTG) | yfdI | CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase |
* | NC_000913 | 2,470,411 | 0 | A | T | 41.9% | 23.9 / 16.5 | 31 | L427F (TTA→TTT) | yfdI | CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase |
* | NC_000913 | 4,220,016 | 0 | A | T | 41.2% | 31.8 / 20.9 | 34 | Y474F (TAT→TTT) | aceK | isocitrate dehydrogenase kinase/phosphatase |
* | NC_000913 | 3,455,669 | 0 | T | G | 41.0% | 41.7 / 10.6 | 39 | I31S (ATT→AGT) | gspC | Type II secretion system protein GspC |
* | NC_000913 | 931,028 | 0 | A | C | 40.5% | 29.5 / 11.2 | 37 | intergenic (‑66/+57) | cydD/trxB | glutathione/L‑cysteine ABC exporter subunit CydD/thioredoxin reductase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 3720633 = | NA (NA) | 5 (0.080) | 5/258 | 5.5 | 6.0% | noncoding (1/1443 nt) | IS150 | repeat region |
? | NC_000913 | = 3721544 | 79 (1.270) | noncoding (912/1443 nt) | IS150 | repeat region | |||||
* | ? | NC_000913 | 2068944 = | NA (NA) | 10 (0.160) | 4/252 | 5.8 | NA | noncoding (1328/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 2070275 | NA (NA) | intergenic (‑95/‑2) | insC‑3/yoeA | CP4‑44 prophage; IS2 insertion element repressor InsA/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA | |||||
* | ? | NC_000913 | 3186094 = | NA (NA) | 10 (0.160) | 4/258 | 5.9 | NA | pseudogene (70/71 nt) | yqiG | putative outer membrane usher protein YqiG |
? | NC_000913 | = 3187426 | NA (NA) | noncoding (1331/1331 nt) | IS2 | repeat region | |||||
* | ? | NC_000913 | 2996360 = | NA (NA) | 5 (0.080) +C |
4/256 | 5.9 | NA | pseudogene (1/399 nt) | ygeO | putative uncharacterized protein YgeO |
? | NC_000913 | = 2997691 | NA (NA) | noncoding (1/1331 nt) | IS2 | repeat region | |||||
* | ? | NC_000913 | = 2070271 | NA (NA) | 10 (0.160) | 4/258 | 5.9 | NA | noncoding (1/1331 nt) | IS2 | repeat region |
? | NC_000913 | 2996359 = | NA (NA) | pseudogene (2/399 nt) | ygeO | putative uncharacterized protein YgeO | |||||
* | ? | NC_000913 | 2068941 = | NA (NA) | 9 (0.140) | 4/258 | 5.9 | NA | noncoding (1331/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 2070273 | NA (NA) | intergenic (‑93/‑4) | insC‑3/yoeA | CP4‑44 prophage; IS2 insertion element repressor InsA/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA | |||||
* | ? | NC_000913 | 3406402 = | 63 (1.010) | 3 (0.050) | 3/234 | 6.1 | 4.8% | coding (486/1350 nt) | accC | biotin carboxylase |
? | NC_000913 | = 3406398 | 63 (1.120) | coding (482/1350 nt) | accC | biotin carboxylase |