breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 73,871 (C)6→7 coding (651/1611 nt) thiP ← thiamine ABC transporter membrane subunit
RA 133,472 C→T L620L (CTG→TTG)  acnB → hypothetical protein
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 341,825 C→T A234V (GCG→GTG)  yahJ → putative deaminase with metallo‑dependent hydrolase domain
RA 613,353 C→T A206V (GCG→GTG)  fes → enterochelin esterase
RA 752,762 C→T A12T (GCC→ACC)  ybgD ← putative fimbrial protein YbgD
RA 827,678 C→T R435Q (CGG→CAG)  ybhF ← putative ABC exporter ATP binding subunit
RA 915,117 C→T intergenic (‑260/+235) lysO ← / ← aqpZ L‑lysine exporter/water channel AqpZ
RA 916,877 A→G G135G (GGA→GGG ybjD → DUF2813 domain‑containing protein YbjD
RA 1,074,174 A→G intergenic (‑163/‑68) rutA ← / → rutR pyrimidine oxygenase/DNA‑binding transcriptional dual regulator RutR
RA 1,157,593 C→T intergenic (+19/‑276) ycfH → / → ptsG putative metal‑dependent hydrolase YcfH/glucose‑specific PTS enzyme IIBC component
RA 1,178,828 A→G N79S (AAT→AGT)  nagK → N‑acetyl‑D‑glucosamine kinase
RA 1,341,896 T→C intergenic (+169/‑25) lapB → / → pyrF lipopolysaccharide assembly protein B/orotidine‑5'‑phosphate decarboxylase
RA 1,594,256 (C)8→9 pseudogene (3831/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,659,963 C→T A632V (GCA→GTA)  ynfE → putative selenate reductase YnfE
RA 1,767,648 G→A L346L (CTC→CTT ydiJ ← putative FAD‑linked oxidoreductase
RA 1,917,265 G→A L336L (CTG→CTA yebS → intermembrane transport protein YebS
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,177,604 G→A R321C (CGT→TGT)  fbaB ← fructose‑bisphosphate aldolase class I
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,669,722 G→A G231S (GGC→AGC)  hcaE → putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha
RA 2,872,528 G→A A98V (GCG→GTG)  ftsB ← cell division protein FtsB
RA 3,129,826 T→C Q130Q (CAA→CAG yghO ← putative DNA‑binding transcriptional regulator YghO
RA 3,149,851 G→A A64T (GCT→ACT)  yghA → NADP(+)‑dependent aldehyde reductase
RA 3,367,999 C→T A58V (GCC→GTC)  yhcG → DUF1016 domain‑containing protein YhcG
RA 3,377,215 A→G L69P (CTG→CCG)  sspA ← stringent starvation protein A
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,617,735 G→A V241I (GTT→ATT)  nikD → Ni(2(+)) ABC transporter ATP binding subunit NikD
JC 4,181,666 (CTTTGTTATCAACGGTAC)1→2 coding (422/4029 nt) rpoB → RNA polymerase subunit beta
RA 4,185,894 C→T A182V (GCG→GTG)  rpoC → RNA polymerase subunit beta'
RA 4,200,155 (C)6→7 intergenic (+61/‑126) yjaG → / → hupA conserved protein YjaG/DNA‑binding protein HU‑alpha
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
JC 4,397,629 (GCTGGC)3→4 coding (218/1848 nt) mutL → DNA mismatch repair protein MutL
RA 4,505,087 A→G T344A (ACC→GCC)  yjhB → putative sialic acid transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423759–3424234 3424532–3424239 6–774 24 [22] [21] 25 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3486111 3486773 663 26 [20] [19] 24 crp crp

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =74 (0.960)29 (0.430) 26/224 1.1 31.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 62 (0.920)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)71 (0.960) 53/248 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein