breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 239 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT60.8% 5.9 / 19.8 57intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913454,9260GT56.2% 6.7 / 19.7 55intergenic (+137/‑207)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009132,177,2340CA55.6% 2.2 / 42.5 65intergenic (‑30/+278)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913707,9810CG55.3% 21.2 / 53.9 52intergenic (+224/‑353)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_000913454,9060GT53.7% 15.4 / 39.0 51intergenic (+117/‑227)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009131,097,0920TC53.6% ‑4.1 / 23.5 32intergenic (+263/+473)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009131,097,1090AT51.4% 0.7 / 24.7 38intergenic (+280/+456)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009132,470,4360CA51.3% 32.4 / 49.5 41Q436K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009134,283,2280TG51.2% ‑4.0 / 14.4 51intergenic (‑153/+25)yjcF/actPpentapeptide repeat‑containing protein YjcF/acetate/glycolate:cation symporter
*NC_000913454,9190GT51.0% 31.7 / 54.1 57intergenic (+130/‑214)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009134,082,6970AC50.0% 0.0 / 13.3 39P21P (CCT→CCGfdoHformate dehydrogenase O subunit beta
*NC_000913454,9330TA47.3% 11.7 / 29.8 56intergenic (+144/‑200)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009132,177,2550GT46.0% 10.8 / 44.7 63intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009134,283,2260GC46.0% 47.3 / 20.8 50intergenic (‑151/+27)yjcF/actPpentapeptide repeat‑containing protein YjcF/acetate/glycolate:cation symporter
*NC_0009132,470,4270CA45.2% 48.2 / 15.0 44Q433K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_000913298,5570AC44.1% 27.1 / 23.3 69L57R (CTC→CGC) paoDmolybdenum cofactor insertion chaperone for PaoABC
*NC_0009131,816,1330AC43.5% 35.3 / 12.8 52intergenic (+5/+253)katE/chbGcatalase II/chitin disaccharide deacetylase
*NC_000913707,9760TG42.3% 24.5 / 20.6 52intergenic (+219/‑358)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009131,636,4420CG41.4% 62.4 / 26.2 61intergenic (‑75/‑314)nohA/ynfOQin prophage; putative prophage DNA‑packaging protein NohA/Qin prophage; protein YnfO
*NC_0009131,636,4470CG41.1% 68.0 / 21.2 60intergenic (‑80/‑309)nohA/ynfOQin prophage; putative prophage DNA‑packaging protein NohA/Qin prophage; protein YnfO

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2288919 =NA (NA)51 (0.460) 22/256 3.2 34.7% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4118287 96 (0.870)intergenic (‑197/‑228) glpF/zapB glycerol facilitator/cell division factor ZapB
* ? NC_000913 = 3653230NA (NA)12 (0.110) 10/256 6.0 11.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 4118287 96 (0.870)intergenic (‑197/‑228) glpF/zapB glycerol facilitator/cell division factor ZapB
* ? NC_000913 = 2067353NA (NA)9 (0.080) 7/250 7.0 8.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 4118287 96 (0.870)intergenic (‑197/‑228) glpF/zapB glycerol facilitator/cell division factor ZapB
* ? NC_000913 4542682 =94 (0.850)5 (0.050) 5/238 7.7 5.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 100 (0.970)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 2066159 =NA (NA)7 (0.060) 5/250 8.0 6.9% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4118287 96 (0.870)intergenic (‑197/‑228) glpF/zapB glycerol facilitator/cell division factor ZapB
* ? NC_000913 781369 =123 (1.110)27 (0.250)
+GAG
4/250 8.5 24.0% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 52 (0.470)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 781369 =123 (1.110)27 (0.250)
+G
4/254 8.6 22.0% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521128 70 (0.630)intergenic (+2/‑45) valX/valY tRNA‑Val/tRNA‑Val
* ? NC_000913 = 1419335148 (1.330)3 (0.030) 3/238 8.8 2.2% coding (122/135 nt) ydaG Rac prophage; uncharacterized protein YdaG
?NC_000913 = 4257134 125 (1.210)coding (20/609 nt) lexA DNA‑binding transcriptional repressor LexA
* ? NC_000913 = 126678998 (0.880)5 (0.050) 3/234 8.8 4.7% coding (696/834 nt) prmC protein‑(glutamine‑N(5)) methyltransferase
?NC_000913 3548869 = 115 (1.130)coding (1202/2085 nt) malQ 4‑alpha‑glucanotransferase
* ? NC_000913 = 76258295 (0.860)3 (0.030) 3/254 9.2 3.1% coding (1061/1218 nt) sucB dihydrolipoyltranssuccinylase
?NC_000913 2730131 = NA (NA)noncoding (1027/1542 nt) rrsG 16S ribosomal RNA