Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,424,210 G→A 5.6% A171T (GCC→ACC)  ulaF → L‑ribulose‑5‑phosphate 4‑epimerase UlaF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,2100GA 5.6% 242.2 / 3.3 71A171T (GCC→ACC) ulaFL‑ribulose‑5‑phosphate 4‑epimerase UlaF
Reads supporting (aligned to +/- strand):  ref base G (30/37);  new base A (2/2);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCAT  >  NC_000913/4424074‑4424346
                                                                                                                                        |                                                                                                                                        
gtgtACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCg                                                                                                                                          <  4:48407/137‑1 (MQ=255)
gtgtACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCg                                                                                                                                          <  5:140926/137‑1 (MQ=255)
  gtACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGcc                                                                                                                                        >  7:146470/1‑137 (MQ=255)
   tACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCt                                                                                                                                       <  2:237198/137‑1 (MQ=255)
       cgcgGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTgggg                                                                                                                                   >  4:150232/1‑137 (MQ=255)
             ttAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCACCTGGGGGa                                                                                                                                 >  2:79634/1‑133 (MQ=255)
             ttAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCACCTGGGGGa                                                                                                                                 >  2:24973/1‑133 (MQ=255)
                 gCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCt                                                                                                                         <  2:151225/137‑1 (MQ=255)
                 gCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCACCTGGGGGAAAGATGCt                                                                                                                         <  1:79634/137‑1 (MQ=255)
                 gCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCACCTGGGGGAAAGATGCt                                                                                                                         <  1:24973/137‑1 (MQ=255)
                   gaagaaGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCa                                                                                                                       <  8:110226/137‑1 (MQ=255)
                      gaagaGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGa                                                                                                                    <  8:146470/137‑1 (MQ=255)
                      gaagaGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGa                                                                                                                    <  5:73206/137‑1 (MQ=255)
                      gaagaGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGa                                                                                                                    >  4:188019/1‑137 (MQ=255)
                       aagaGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGAt                                                                                                                   <  4:249909/137‑1 (MQ=255)
                        agagGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCACTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                                                                                  >  1:71101/1‑137 (MQ=255)
                             tGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTg                                                                                                             <  6:246440/137‑1 (MQ=255)
                             tGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTg                                                                                                             <  3:150232/137‑1 (MQ=255)
                                  ggCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATaa                                                                                                        <  8:36152/137‑1 (MQ=255)
                                      aGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAAcgcg                                                                                                    >  7:266118/1‑137 (MQ=255)
                                      aGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCACTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAAcgcg                                                                                                    <  7:213088/137‑1 (MQ=255)
                                        tATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggt                                                                                                  >  7:50275/1‑137 (MQ=255)
                                         aTGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtg                                                                                                 >  6:232160/1‑137 (MQ=255)
                                         aTGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtg                                                                                                 >  3:94781/1‑137 (MQ=255)
                                              ctgaacACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGATGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGAt                                                                                            >  1:178870/1‑137 (MQ=255)
                                                   cacCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                                                                                  <  7:115908/110‑1 (MQ=255)
                                                   cacCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                                                                                  >  8:115908/1‑110 (MQ=255)
                                                   cacCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATgg                                                                                          >  5:111021/1‑134 (MQ=255)
                                                   cacCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATgg                                                                                          >  8:182551/1‑134 (MQ=255)
                                                   cacCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATgg                                                                                          >  8:245950/1‑134 (MQ=255)
                                                   cacCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATgg                                                                                          >  8:24686/1‑134 (MQ=255)
                                                   cacCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATgg                                                                                          >  3:32373/1‑134 (MQ=255)
                                                   cacCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATgg                                                                                          >  4:16204/1‑134 (MQ=255)
                                                   cacCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCATTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGAGGTGGTGATgg                                                                                          >  4:72523/1‑134 (MQ=255)
                                                   cacCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCAAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaag                                                                                       <  2:154095/137‑1 (MQ=255)
                                                   cacCGGCAAAGTCATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGCAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCTGTGCACAACGAGGAGGTGATgg                                                                                          >  6:78461/1‑134 (MQ=255)
                                                        gCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                                                                                  <  7:37567/105‑1 (MQ=255)
                                                        gCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                                                                                  >  8:37567/1‑105 (MQ=255)
                                                        gCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                                                                                  >  2:113667/1‑137 (MQ=255)
                                                        gCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                                                                                  >  2:111142/1‑137 (MQ=255)
                                                        gCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                                                                                  >  8:40115/1‑137 (MQ=255)
                                                            aGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGGTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa                                                                              <  8:70140/137‑1 (MQ=255)
                                                                 ttATCGACACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATg                                                                         <  7:8662/137‑1 (MQ=255)
                                                                    tCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCg                                                                      >  7:236892/1‑137 (MQ=255)
                                                                      gAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                                    <  6:101777/137‑1 (MQ=255)
                                                                               ggCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGcc                                                             <  3:16204/135‑1 (MQ=255)
                                                                               ggCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGcc                                                             <  8:266118/135‑1 (MQ=255)
                                                                                   aCGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGc                                                       <  3:100417/137‑1 (MQ=255)
                                                                                   aCGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGc                                                       <  4:136400/137‑1 (MQ=255)
                                                                                   aCGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTGCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGc                                                       <  1:170800/137‑1 (MQ=255)
                                                                                   aCGCCGAGCCGCTGCACACGCCGGGAATTGCGGTGTATCAGCACGGGCCGTGCGCCTGGGGGAAAGATGCTCACGATGCGGCGCATAACGCGGTGGGGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGc                                                       <  1:59844/137‑1 (MQ=255)
                                                                                     gCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt                                                     <  6:142730/137‑1 (MQ=255)
                                                                                     gCCGAGCCACTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGTTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt                                                     <  2:71101/137‑1 (MQ=255)
                                                                                        gAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTaa                                                  <  2:81248/137‑1 (MQ=255)
                                                                                           ccGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGc                                                       >  8:149120/1‑129 (MQ=255)
                                                                                           ccGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGc                                                       <  7:149120/129‑1 (MQ=255)
                                                                                           ccGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAAccc                                               >  4:117733/1‑137 (MQ=255)
                                                                                            cGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCca                                              <  6:139718/137‑1 (MQ=255)
                                                                                             gCTGCATACGCCGGGAATTGTGGGGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac                                             <  2:248244/137‑1 (MQ=255)
                                                                                                   tACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa                                       <  6:53402/137‑1 (MQ=255)
                                                                                                   tACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa                                       <  7:148001/137‑1 (MQ=255)
                                                                                                   tACGCCGGGAATTGTGGTGAATCAGCACGGGCCGTGCGCCTGGGGGAAATATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa                                       <  5:171813/137‑1 (MQ=255)
                                                                                                     cGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAAt                                     >  4:126313/1‑137 (MQ=255)
                                                                                                          ggAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACAt                                >  8:250198/1‑137 (MQ=255)
                                                                                                             aTTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGa                             <  7:182551/137‑1 (MQ=255)
                                                                                                                          aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtgat                 >  7:63207/1‑136 (MQ=255)
                                                                                                                             aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGaa              >  6:224022/1‑136 (MQ=255)
                                                                                                                              cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATa            <  4:32373/137‑1 (MQ=255)
                                                                                                                              cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATa            <  8:63207/137‑1 (MQ=255)
                                                                                                                              cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGGGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATa            <  5:118186/137‑1 (MQ=255)
                                                                                                                                  ccGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAaca        >  3:176856/1‑137 (MQ=255)
                                                                                                                                   cGTTCGCCGGGGGGAAAGAGGCTCACGATGCTGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAacac       <  7:194296/137‑1 (MQ=255)
                                                                                                                                    gTTCGCGTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACt      <  2:247501/137‑1 (MQ=255)
                                                                                                                                        gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTACCCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCAt  >  7:18880/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCAT  >  NC_000913/4424074‑4424346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑