breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 89 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT69.6% 28.0 / 15.0 61intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,177,2550GT60.9% 17.6 / 44.9 69intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,470,4270CA58.6% 7.4 / 28.4 30Q433K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,177,2340CA51.4% 1.0 / 30.4 59intergenic (‑30/+278)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913454,8710GT50.0% 2.3 / 10.2 22intergenic (+82/‑262)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_000913707,9760TG47.2% 17.2 / 16.2 36intergenic (+219/‑358)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009132,177,2210TG46.6% 23.8 / 35.5 58intergenic (‑17/+291)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913707,9810CG45.8% 22.0 / 13.7 36intergenic (+224/‑353)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009132,177,2280CG44.7% 34.1 / 21.8 58intergenic (‑24/+284)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009133,004,9460GT44.2% 25.6 / 22.2 43Q281K (CAA→AAA) ygeVputative sigma(54)‑dependent transcriptional regulator YgeV
*NC_0009133,455,6800GT42.2% 41.8 / 16.7 45V35L (GTG→TTG) gspCType II secretion system protein GspC
*NC_0009134,378,9730GC41.4% 2.0 / 12.0 29intergenic (‑29/+34)ampC/frdDbeta‑lactamase/fumarate reductase membrane protein FrdD
*NC_0009133,455,6690TG41.1% 59.1 / 20.4 56I31S (ATT→AGT) gspCType II secretion system protein GspC
*NC_0009131,708,3260GT40.4% 58.9 / 12.7 53E470* (GAA→TAA) rsxCSoxR [2Fe‑2S] reducing system protein RsxC
*NC_0009132,177,2670CA39.7% 97.6 / 19.3 77intergenic (‑63/+245)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009131,997,0670GT38.7% 35.7 / 10.9 31Q250K (CAA→AAA) tcyNcystine ABC transporter ATP binding subunit
*NC_0009133,815,8090GC38.7% 126.6 / 68.6 93intergenic (‑41/+54)pyrE/rphorotate phosphoribosyltransferase/truncated RNase PH
*NC_0009131,879,8460TG38.3% 50.0 / 19.0 47T35P (ACG→CCG) yeaRDUF1971 domain‑containing protein YeaR
*NC_0009133,455,6770AT38.0% 46.0 / 19.4 50N34Y (AAT→TAT) gspCType II secretion system protein GspC
*NC_000913707,9840CG36.4% 34.1 / 10.6 35intergenic (+227/‑350)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381258 =NA (NA)12 (0.160) 7/254 5.3 NA pseudogene (3/417 nt) yaiX putative acyltransferase, N‑terminal fragment
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region
* ? NC_000913 4498181 =NA (NA)13 (0.170) 7/256 5.4 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD‑6/yjgX KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
* ? NC_000913 3787895 =165 (2.160)4 (0.060) 4/236 6.3 2.5% coding (1058/1260 nt) envC murein hydrolase activator EnvC
?NC_000913 = 3787891 156 (2.220)coding (1054/1260 nt) envC murein hydrolase activator EnvC
* ? NC_000913 4010494 =69 (0.900)4 (0.050) 4/254 6.7 5.1% coding (295/801 nt) yigL phosphosugar phosphatase YigL
?NC_000913 4239750 = 79 (1.040)intergenic (+20/+27) yjbH/yjbT YjbH family protein/PF17089 family protein YjbT
* ? NC_000913 3736656 =266 (3.480)3 (0.040) 3/236 6.9 1.2% coding (522/825 nt) bax putative glycoside hydrolase Bax
?NC_000913 = 3736652 246 (3.490)coding (526/825 nt) bax putative glycoside hydrolase Bax
* ? NC_000913 4542682 =70 (0.920)3 (0.040) 3/238 7.0 3.8% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 86 (1.210)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit