breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 67 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT71.4% 16.1 / 14.0 45intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,177,2340CA57.1% ‑0.3 / 17.4 42intergenic (‑30/+278)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913454,9060GT56.5% 4.3 / 15.0 28intergenic (+117/‑227)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_000913707,9810CG53.3% 2.3 / 19.3 25intergenic (+224/‑353)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009132,470,4310GA53.3% ‑4.7 / 13.8 20R434K (AGA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,177,2550GT52.2% ‑1.0 / 31.6 46intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009133,407,9700CG48.1% 19.3 / 12.6 27L122V (CTT→GTT) panFpantothenate:Na(+) symporter
*NC_0009132,470,4110AT45.5% 10.8 / 11.2 22L427F (TTA→TTTyfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_000913238,5350GT42.9% 27.2 / 14.4 60H68N (CAT→AAT) ykfMuncharacterized protein YkfM
*NC_0009134,220,0180GT42.2% 46.4 / 16.8 45D475Y (GAT→TAT) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_0009133,314,1960GC41.4% 31.6 / 11.8 30R607G (CGT→GGT) infBtranslation initiation factor IF‑2
*NC_0009133,455,6690TG41.1% 46.0 / 33.2 56I31S (ATT→AGT) gspCType II secretion system protein GspC
*NC_0009132,470,4050TG40.9% 12.5 / 11.7 22V425V (GTT→GTGyfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,177,2280CG40.7% 5.2 / 17.3 42intergenic (‑24/+284)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009131,636,4420CG40.0% 27.9 / 12.1 30intergenic (‑75/‑314)nohA/ynfOQin prophage; putative prophage DNA‑packaging protein NohA/Qin prophage; protein YnfO
*NC_0009132,177,2210TG40.0% 29.6 / 16.8 40intergenic (‑17/+291)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,470,4720GT39.3% 31.4 / 12.9 30intergenic (+10/+605)yfdI/yfdKCPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase/CPS‑53 (KpLE1) prophage; putative tail fiber assembly protein YfdK
*NC_0009133,955,6160TG38.2% 31.4 / 10.4 34F96V (TTT→GTT) ilvAthreonine deaminase
*NC_0009133,455,6770AT37.0% 41.6 / 28.1 54N34Y (AAT→TAT) gspCType II secretion system protein GspC
*NC_0009133,455,6800GT36.7% 50.4 / 18.3 50V35L (GTG→TTG) gspCType II secretion system protein GspC

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4498180 =NA (NA)18 (0.310) 6/254 4.4 NA intergenic (+240/‑92) intB/insC‑6 KpLE2 phage‑like element; putative integrase/KpLE2 phage‑like element; IS2 insertion element repressor InsA
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 1467909 =NA (NA)10 (0.170) 6/254 4.4 NA pseudogene (2518/2518 nt) ydbA putative outer membrane protein, N‑terminal fragment
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 381258 =NA (NA)7 (0.120) 5/252 4.7 NA pseudogene (3/417 nt) yaiX putative acyltransferase, N‑terminal fragment
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region
* ? NC_000913 4498181 =NA (NA)8 (0.140) 5/254 4.8 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD‑6/yjgX KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
* ? NC_000913 4542682 =57 (0.990)3 (0.060) 3/236 5.4 5.4% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 53 (0.990)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 360774738 (0.660)3 (0.050) 3/252 5.7 6.5% coding (1297/2199 nt) zntA Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
?NC_000913 4329149 = 49 (0.850)coding (2015/2229 nt) crfC clamp‑binding sister replication fork colocalization protein