breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 153 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT71.7% 23.4 / 11.0 49intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,470,4270CA63.0% ‑4.0 / 35.9 50Q433K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,177,2550GT58.9% 3.0 / 41.4 56intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009131,097,1090AT56.7% ‑4.9 / 18.7 31intergenic (+280/+456)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_000913707,9760TG55.6% 5.7 / 43.5 54intergenic (+219/‑358)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009133,491,6930AC55.0% 6.4 / 18.5 20intergenic (‑73/+32)yhfG/ppiADUF2559 domain‑containing protein YhfG/peptidyl‑prolyl cis‑trans isomerase A
*NC_0009132,177,2210TG54.9% 7.9 / 42.9 51intergenic (‑17/+291)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913454,9260GT52.9% 10.0 / 31.4 49intergenic (+137/‑207)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_000913454,9190GT52.2% 33.2 / 55.7 51intergenic (+130/‑214)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009132,449,1940AC52.2% ‑0.2 / 13.8 28intergenic (+37/+34)smrB/yfcOputative endonuclease SmrB/DUF2544 domain‑containing protein YfcO
*NC_000913454,9060GT51.2% 4.5 / 31.9 50intergenic (+117/‑227)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009131,097,0920TC50.0% ‑1.3 / 18.4 30intergenic (+263/+473)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009132,177,2280CT50.0% ‑5.9 / 40.6 52intergenic (‑24/+284)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,470,4360CA48.9% 28.5 / 15.3 48Q436K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,470,4110AT47.1% 27.7 / 31.9 51L427F (TTA→TTTyfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,177,2340CT44.8% 2.8 / 28.5 51intergenic (‑30/+278)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913454,8730CG42.9% 27.2 / 28.5 57intergenic (+84/‑260)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_000913708,0040CA42.9% 60.1 / 12.5 57intergenic (+247/‑330)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_000913707,9810CG42.4% 29.4 / 21.7 56intergenic (+224/‑353)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009132,470,4310GT42.4% 20.3 / 12.8 49R434I (AGA→ATA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4498181 =NA (NA)11 (0.120) 6/256 6.3 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD‑6/yjgX KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
* ? NC_000913 2996360 =NA (NA)8 (0.090)
+C
6/254 6.3 NA pseudogene (1/399 nt) ygeO putative uncharacterized protein YgeO
?NC_000913 = 2997691 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 2068941 =NA (NA)13 (0.140) 6/256 6.3 NA noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 2070273 NA (NA)intergenic (‑93/‑4) insC‑3/yoeA CP4‑44 prophage; IS2 insertion element repressor InsA/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA
* ? NC_000913 381258 =NA (NA)14 (0.150) 6/254 6.3 NA pseudogene (3/417 nt) yaiX putative acyltransferase, N‑terminal fragment
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region
* ? NC_000913 731157 =102 (1.110)3 (0.030) 3/248 7.8 2.7% coding (1575/4194 nt) rhsC rhs element protein RhsC
?NC_000913 = 855588 117 (1.320)coding (157/921 nt) ldtB L,D‑transpeptidase YbiS
* ? NC_000913 628293 =101 (1.100)3 (0.030) 3/250 7.8 3.4% coding (600/858 nt) entB enterobactin synthase component B
?NC_000913 663005 = 74 (0.830)coding (959/1212 nt) dacA D‑alanyl‑D‑alanine carboxypeptidase DacA
* ? NC_000913 = 359395566 (0.720)3 (0.030) 3/254 7.9 4.6% coding (252/768 nt) livG branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivG
?NC_000913 = 3595143 59 (0.650)coding (338/1278 nt) livM branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM
* ? NC_000913 = 315276785 (0.930)3 (0.030) 3/256 7.9 3.2% coding (532/1188 nt) metC cystathionine beta‑lyase/L‑cysteine desulfhydrase
?NC_000913 3152773 = 94 (1.030)coding (538/1188 nt) metC cystathionine beta‑lyase/L‑cysteine desulfhydrase