Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,297,596 C→T 9.0% E176K (GAA→AAA)  adhE ← aldehyde‑alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,297,5960CT 9.0% 130.9 / 2.7 47E176K (GAA→AAA) adhEaldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (18/22);  new base T (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTACGG  >  NC_000913/1297477‑1297732
                                                                                                                       |                                                                                                                                        
gCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTATGGGTGGTGCATCAGTGCCTTAGACACTTAAACAGAAGGTTgatag                                                                                                                          >  2:22570/1‑134 (MQ=255)
  ccGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgatcgatc                                                                                                                       >  2:92371/1‑137 (MQ=255)
   cgcgCCTACACCGATAGCTGGTTTACAGGAGCTGTATGCGGCTTTAACCATTCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATcc                                                                                                                      <  1:169452/137‑1 (MQ=255)
    gcgcCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTATACAGTTCAACAGAAGGTTGATCGATCCacc                                                                                                                   >  6:112562/1‑137 (MQ=255)
                  aGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGCGGATCAGGGTGGTGTCTGGGTGGTGCATCAGTGGGGTAGACAGGTCAACATAAGGTTGATCGATCCAGCCGCTCAGATCtt                                                                                                       >  4:59975/1‑137 (MQ=255)
                  aGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGCTCAGGTTGATGTGTGGGTGGTGGATGAGTTCCGTATCCAGTTGAAAAGAAGTTTGATGGTTACAGCCGCTCAGagagt                                                                                                       >  2:294525/1‑133 (MQ=255)
                  aGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTTATTTCTGGGTGGTGCATAAGTGCGGTTGACAGTTAAACCGAAGGTTTATCTATCCAGCCGAGCAGATCtt                                                                                                       >  8:17088/1‑137 (MQ=255)
                  aGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTTGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATAtt                                                                                                       >  8:15510/1‑137 (MQ=255)
                  aGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCtt                                                                                                       >  8:38682/1‑137 (MQ=255)
                  aGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGAAGTCAGGGTGGTGCATCAGTGGGTTAGACACTTCAACAGAACGTTTATCGATCCCGCCGAACAGATCtt                                                                                                       >  4:84694/1‑137 (MQ=255)
                            ccGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCAc                                                                                              <  1:92371/136‑1 (MQ=255)
                             cGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCg                                                                                            <  8:33110/137‑1 (MQ=255)
                                  cTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGc                                                                                       <  8:265633/137‑1 (MQ=255)
                                        gCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATag                                                                                  <  4:83644/136‑1 (MQ=255)
                                              ttAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTATGGGTGGTGCATCAGTGCGTTAGACAGTTTAACAGAACGTTTATCGATCCAGCCGATCAGATAATTAGGAGCACCCGCAGCGATAACAGccag                                                                           >  8:165576/1‑135 (MQ=255)
                                              ttAACCATGCCCGGACCACCAGTCGCGAGGATAAGGTTGATGTATGGGTGGTGCATCAGTGCGTTAGACAGTTTAACAGAAGGTTGATAGATCCAGCCGATCAGATCTTTAGGAGCACCCGCCGCGATAgccgccag                                                                           >  8:161705/1‑135 (MQ=255)
                                                 aCCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCa                                                                         <  8:51595/136‑1 (MQ=255)
                                                           gACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGc                                                            <  6:112257/139‑1 (MQ=255)
                                                           gACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGCTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCa                                                              <  8:150958/137‑1 (MQ=255)
                                                               accaGTCGCGAGGATCAGGTTGATGTCTGGGTGGTTCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCg                                                          <  8:219726/137‑1 (MQ=255)
                                                               accaGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCg                                                          <  1:22570/137‑1 (MQ=255)
                                                               accaGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCt                                                        <  5:199803/139‑1 (MQ=255)
                                                                           gATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTtggtgg                                              <  3:144783/137‑1 (MQ=255)
                                                                             tCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCa                                            >  7:6610/1‑137 (MQ=255)
                                                                                ggTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCt                                         >  4:31289/1‑137 (MQ=255)
                                                                                        tCTGGGTGGTGCATCAGTTCGTTAGACAGATCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCAcg                                 <  3:257189/137‑1 (MQ=255)
                                                                                             gtggtgCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGt                            >  3:213170/1‑137 (MQ=255)
                                                                                             gtggtgCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGt                            >  3:227881/1‑137 (MQ=255)
                                                                                                  gCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCggg                       <  1:81116/137‑1 (MQ=255)
                                                                                                   cATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCggg                       >  3:21110/1‑136 (MQ=255)
                                                                                                   cATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCggg                       <  3:69319/136‑1 (MQ=255)
                                                                                                   cATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGAGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCggg                       >  2:81116/1‑136 (MQ=255)
                                                                                                   cATCAGTGAGTTAGACAGTTCAACAGAGGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGgag                    <  6:56632/139‑1 (MQ=255)
                                                                                                           cGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAAt            >  6:282750/1‑139 (MQ=255)
                                                                                                           cGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAAt            >  6:87830/1‑139 (MQ=255)
                                                                                                             ttAGACAGTTCAACATAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATCTCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAAt            <  3:254788/137‑1 (MQ=255)
                                                                                                             ttAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCgggg                      >  1:215942/1‑127 (MQ=255)
                                                                                                             ttAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCgggg                      <  2:215942/127‑1 (MQ=255)
                                                                                                             ttAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAAt            >  1:140221/1‑137 (MQ=255)
                                                                                                                gACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGc         <  8:50351/137‑1 (MQ=255)
                                                                                                                  cAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCGTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGt       >  7:139507/1‑137 (MQ=255)
                                                                                                                    gTTTAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTa     <  7:165576/137‑1 (MQ=255)
                                                                                                                    gTTTAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTa     <  7:161705/137‑1 (MQ=255)
                                                                                                                    gTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTa     <  7:38682/137‑1 (MQ=255)
                                                                                                                    gTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTa     <  8:19675/137‑1 (MQ=255)
                                                                                                                    gTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTa     <  4:61280/137‑1 (MQ=255)
                                                                                                                     ttCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCCCCGGCAGCGCTAGCAGCCTGCAGAACGATATCAGCCGCTGTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGCGAAGATAATGGCGTTACgg  >  6:135701/1‑139 (MQ=255)
                                                                                                                       cAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTACgg  >  7:238101/1‑137 (MQ=255)
                                                                                                                       cAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTACgg  >  2:117681/1‑137 (MQ=255)
                                                                                                                       cAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTACgg  >  1:74509/1‑137 (MQ=255)
                                                                                                                       |                                                                                                                                        
GCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTACGG  >  NC_000913/1297477‑1297732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑