breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273161,0680AC36.4% ‑5.2 / 11.0 22intergenic (+47/‑149)hrpB/mrcBputative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
*CP0092732,178,9080TG29.5% 112.6 / 23.4 78intergenic (‑128/+95)thiM/rcnRhydoxyethylthiazole kinase/transcriptional repressor of rcnA
*CP009273838,6520TG29.1% 187.7 / 26.3 117F289V (TTT→GTT) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP009273838,6720GT27.9% 226.5 / 23.5 129M295I (ATG→ATTrlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP009273104,0410GT27.9% 92.9 / 27.8 61intergenic (+80/‑151)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
*CP0092732,170,7340GT27.0% 122.4 / 42.1 126intergenic (‑51/+257)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
*CP009273418,9950TG26.0% 131.5 / 10.6 77L341R (CTG→CGG) malZmaltodextrin glucosidase
*CP009273838,6750CA23.4% 238.3 / 24.0 124N296K (AAC→AAArlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP0092734,402,5960TG21.7% 152.4 / 11.8 69V131V (GTT→GTGyjfMDUF1190 family protein
*CP0092732,475,2990CA21.6% 181.6 / 12.2 88M119I (ATG→ATTemrYputative multidrug efflux system
*CP0092732,998,3330TA21.1% 229.3 / 16.2 91Q271H (CAA→CATygeVputative sigma‑54‑interacting transcriptional activator
*CP009273838,6580GT20.0% 221.4 / 11.6 115A291S (GCC→TCC) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 120326168 (0.600)25 (0.250) 16/236 3.2 29.8% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 58 (0.580)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 312934 =98 (0.860)8 (0.070) 8/268 6.2 7.5% intergenic (‑54/‑216) ykgA/ykgQ pseudogene, AraC family;putative regulator; Not classified; putative ARAC‑type regulatory protein/pseudogene, putative dehydrogenase
?CP009273 1462167 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? CP009273 = 31293893 (0.810)11 (0.100) 7/268 6.6 10.6% intergenic (‑58/‑212) ykgA/ykgQ pseudogene, AraC family;putative regulator; Not classified; putative ARAC‑type regulatory protein/pseudogene, putative dehydrogenase
?CP009273 376716 = NA (NA)intergenic (‑1/‑91) yaiX/insC1 pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase/IS2 repressor TnpA
* ? CP009273 281564 =190 (1.660)4 (0.040) 4/252 7.8 2.2% coding (459/1383 nt) yagG CP4‑6 prophage; putative sugar transporter
?CP009273 = 281560 181 (1.690)coding (455/1383 nt) yagG CP4‑6 prophage; putative sugar transporter
* ? CP009273 126812 =110 (0.960)3 (0.030) 3/250 8.4 2.9% coding (936/1854 nt) yacH uncharacterized protein
?CP009273 = 126808 98 (0.920)coding (940/1854 nt) yacH uncharacterized protein
* ? CP009273 3608179 =171 (1.500)3 (0.030) 3/262 8.6 2.1% coding (1153/1575 nt) nikA nickel‑binding, heme‑binding periplasmic protein
?CP009273 4540982 = 116 (1.040)coding (132/1344 nt) gntP fructuronate transporter
* ? CP009273 = 1543776142 (1.240)5 (0.040) 3/262 8.6 3.4% coding (2119/3048 nt) fdnG formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
?CP009273 1543786 = 144 (1.290)coding (2129/3048 nt) fdnG formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
* ? CP009273 = 2309235113 (0.990)3 (0.030) 3/266 8.7 2.6% coding (2269/2673 nt) rcsD phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
?CP009273 2309241 = 112 (0.990)coding (2275/2673 nt) rcsD phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
* ? CP009273 2274542 =77 (0.670)3 (0.030) 3/268 8.8 3.1% coding (432/1761 nt) yejH putative ATP‑dependent DNA or RNA helicase
?CP009273 2308983 = 111 (0.970)coding (2017/2673 nt) rcsD phosphotransfer intermediate protein in two‑component regulatory system with RcsBC