breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 17,673 G→T 19.8% W62L (TGG→TTG)  nhaA → sodium‑proton antiporter
RA 18,787 G→T 9.3% G25C (GGC→TGC)  nhaR → transcriptional activator of nhaA
RA 49,981 G→T 8.6% P53P (CCG→CCT folA → dihydrofolate reductase
RA 141,158 C→A 8.2% A32D (GCC→GAC)  yadI → putative PTS Enzyme IIA
RA 169,472 G→T 14.0% V508V (GTG→GTT fhuB → fused iron‑hydroxamate transporter subunits of ABC superfamily: membrane components
RA 210,646 G→T 8.4% intergenic (‑34/‑132) yaeP ← / → yaeQ UPF0253 family protein/PDDEXK superfamily protein
RA 216,968 Δ1 bp 11.1% coding (448/816 nt) metQ ← DL‑methionine transporter subunit
RA 216,969 Δ1 bp 11.1% coding (447/816 nt) metQ ← DL‑methionine transporter subunit
RA 216,970 Δ1 bp 11.1% coding (446/816 nt) metQ ← DL‑methionine transporter subunit
RA 221,373:1 +G 8.0% noncoding (1116/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,380 T→A 7.5% noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,390 Δ1 bp 7.8% noncoding (1133/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,391 G→A 7.8% noncoding (1134/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,393 C→T 7.8% noncoding (1136/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,395 G→A 7.9% noncoding (1138/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,397 C→T 7.9% noncoding (1140/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,398 C→T 8.1% noncoding (1141/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,402 A→C 8.4% noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,407 A→T 8.5% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 8.4% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 9.0% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 15.6% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 15.9% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,455 G→A 20.0% noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,464 G→C 22.8% noncoding (1207/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,471 C→T 23.8% noncoding (1214/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,476 A→T 27.8% noncoding (1219/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 237,957 C→A 6.5% G612W (GGG→TGG)  fadE ← acyl coenzyme A dehydrogenase
RA 262,312 G→T 13.2% pseudogene (174/222 nt) ykfL ← pseudogene, CP4‑6 putative prophage remnant;Phage or Prophage Related
RA 262,318 G→A 10.4% pseudogene (168/222 nt) ykfL ← pseudogene, CP4‑6 putative prophage remnant;Phage or Prophage Related
RA 263,285 G→A 7.8% D144D (GAC→GAT yafZ ← CP4‑6 prophage; conserved protein
RA 263,852 T→A 11.7% T274S (ACG→TCG)  ykfA ← CP4‑6 prophage; putative GTP‑binding protein
RA 263,855 T→A 11.5% T273S (ACC→TCC)  ykfA ← CP4‑6 prophage; putative GTP‑binding protein
RA 263,856 G→A 11.6% R272R (CGC→CGT ykfA ← CP4‑6 prophage; putative GTP‑binding protein
RA 263,871 G→A 9.9% V267V (GTC→GTT) ‡ ykfA ← CP4‑6 prophage; putative GTP‑binding protein
RA 263,873 C→T 10.0% V267I (GTC→ATC) ‡ ykfA ← CP4‑6 prophage; putative GTP‑binding protein
RA 263,876 C→T 10.4% A266T (GCC→ACC)  ykfA ← CP4‑6 prophage; putative GTP‑binding protein
RA 274,938 T→A 9.5% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 293,932 C→A 7.0% G169V (GGC→GTC)  paoD ← moco insertion factor for PaoABC aldehyde oxidoreductase
RA 317,275 G→T 10.2% intergenic (+483/‑44) rclR → / → ykgE reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component
RA 356,558 C→A 9.6% P286T (CCG→ACG)  cynX → putative cyanate transporter
RA 372,887:1 +A 11.5% intergenic (+120/+104) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,888 C→G 10.2% intergenic (+121/+103) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 392,187 G→T 10.5% G31G (GGG→GGT sbmA → microcin B17 transporter
RA 392,604 G→T 7.8% M170I (ATG→ATT sbmA → microcin B17 transporter
RA 405,300 A→G 7.4% V59A (GTT→GCT)  rdgC ← nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function
RA 458,913 G→T 10.0% G515C (GGT→TGT)  ppiD → periplasmic folding chaperone, has an inactive PPIase domain
RA 473,912 C→A 7.8% K54N (AAG→AAT ylaB ← putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 479,431 G→T 16.0% I143I (ATC→ATA acrB ← multidrug efflux system protein
RA 488,500 G→A 17.4% R318H (CGC→CAC)  dnaX → DNA polymerase III/DNA elongation factor III, tau and gamma subunits
RA 491,877 C→A 10.5% T434T (ACC→ACA htpG → protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase
RA 511,328 G→T 10.0% intergenic (‑99/‑47) qmcA ← / → fetA PHB domain membrane‑anchored putative protease/iron exporter, ATP‑binding subunit, ABC transporter FetAB subunit; peroxide resistance protein
RA 521,092 C→T 5.8% S792S (AGC→AGT rhsD → Rhs family putative polymorphic toxin
RA 543,418 C→T 10.2% A428V (GCC→GTC)  fdrA → putative NAD(P)‑binding acyl‑CoA synthetase
RA 546,431 G→T 7.4% V179V (GTG→GTT ybcF → putative carbonate kinase
RA 569,275 T→A 14.3% D30E (GAT→GAA ylcG → uncharacterized protein, DLP12 prophage
RA 569,578 G→T 22.2% C56F (TGC→TTC)  quuD → DLP12 prophage; putative antitermination protein
RA 573,883 G→C 8.1% T107T (ACG→ACC
R34P (CGG→CCG) 
rzpD →
rzoD →
DLP12 prophage; putative murein endopeptidase
DLP12 prophage; putative lipoprotein
RA 577,301 T→C 9.0% pseudogene (186/438 nt) tfaD → pseudogene, DLP12 prophage; tail fiber assembly protein family;Phage or Prophage Related
RA 632,334 G→T 26.7% V247V (GTC→GTA ybdO ← putative DNA‑binding transcriptional regulator
RA 639,119 C→A 14.6% R102L (CGC→CTC)  rnk ← regulator of nucleoside diphosphate kinase
RA 645,155 G→A 10.5% A264A (GCC→GCT citE ← citrate lyase, citryl‑ACP lyase (beta) subunit
RA 694,863 A→T 8.5% intergenic (‑230/+167) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 694,871 A→C 12.5% intergenic (‑238/+159) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 694,872 T→G 9.3% intergenic (‑239/+158) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 703,862 A→G 9.7% M25V (ATG→GTG)  chiP → chitoporin, uptake of chitosugars
RA 726,319 G→C 5.6% A427A (GCG→GCC rhsC → Rhs family putative polymorphic toxin
RA 726,453 G→A 9.9% S472N (AGC→AAC)  rhsC → Rhs family putative polymorphic toxin
RA 727,171 T→G 6.9% N711K (AAT→AAG rhsC → Rhs family putative polymorphic toxin
RA 727,174 C→T 6.1% G712G (GGC→GGT rhsC → Rhs family putative polymorphic toxin
RA 727,989 C→A 8.0% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 732,955 G→T 8.2% pseudogene (675/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 732,984 T→C 7.3% pseudogene (704/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 733,018 A→G 5.3% pseudogene (738/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 786,409 G→T 11.7% P26Q (CCG→CAG)  galT ← galactose‑1‑phosphate uridylyltransferase
RA 792,733 C→T 13.9% H242Y (CAT→TAT)  modC → molybdate transporter subunit
RA 832,600 C→A 12.1% P265H (CCC→CAC)  ybiC → putative dehydrogenase
RA 847,414 G→A 10.8% P214S (CCG→TCG)  ybiP ← putative EptAB family phosphoethanolamine transferase, inner membrane protein
RA 851,338 G→T 9.3% intergenic (‑138/‑81) ldtB ← / → ybiT L,D‑transpeptidase linking Lpp to murein/putative transporter subunit of ABC superfamily: ATP‑binding component
RA 865,055 C→T 12.9% F63F (TTC→TTT gsiB → glutathione periplasmic binding protein, ABC superfamily transporter
RA 868,177 G→T 10.0% A278S (GCT→TCT)  gsiD → glutathione transporter, permease component, ABC superfamily
RA 913,047 G→T 9.9% P373P (CCG→CCT ybjD → putative OLD family ATP‑dependent endonuclease; DUF2813 family protein
RA 928,185 C→T 7.8% C45C (TGC→TGT lrp → leucine‑responsive global transcriptional regulator
RA 948,642 G→A 5.4% C123C (TGC→TGT pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 952,720 A→C 14.2% K168T (AAG→ACG)  ycaP → UPF0702 family putative inner membrane protein
RA 1,031,095 G→A 10.5% G192S (GGC→AGC)  hyaC → hydrogenase 1, b‑type cytochrome subunit
RA 1,040,257 C→A 15.4% E335* (GAA→TAA)  gfcE ← putative O‑antigen capsule outer membrane auxillary protein export channel
RA 1,045,283 T→G 10.6% intergenic (‑83/‑6) gfcA ← / → insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
RA 1,066,607 G→T 10.8% C205* (TGC→TGA rutD ← putative aminoacrylate hydrolase, reactive intermediate detoxification
RA 1,095,815 G→T 5.4% A22S (GCT→TCT)  ycdZ → DUF1097 family inner membrane protein
RA 1,112,507 A→T 37.1% Q82L (CAG→CTG)  yceA → putative rhodanese‑related sulfurtransferase
RA 1,252,353 C→T 11.1% G306S (GGC→AGC)  ychF ← catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated
RA 1,282,249 A→G 15.6% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,421 A→G 8.5% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,430 C→T 6.8% intergenic (+448/+92) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,439 A→G 8.2% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,599 G→A 5.9% noncoding (94/171 nt)
P12S (CCA→TCA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein
RA 1,284,941 G→T 16.0% G81C (GGT→TGT)  rssA → putative patatin‑like family phospholipase
RA 1,331,482 C→A 9.5% V465V (GTC→GTA acnA → aconitate hydratase 1
RA 1,374,549 C→A 17.4% R49S (CGC→AGC)  ycjU → beta‑phosphoglucomutase
RA 1,422,060 G→C 6.6% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,132 C→T 7.7% pseudogene (852/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,150 C→T 5.2% pseudogene (834/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,252 A→G 6.8% pseudogene (732/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 5.2% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,744 G→A 7.9% pseudogene (240/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,756 G→C 5.0% pseudogene (228/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,759 A→G 5.0% pseudogene (225/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,762 G→A 5.0% pseudogene (222/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,957 A→G 6.1% pseudogene (27/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,426,820 G→A 22.2% P51P (CCG→CCA tfaR → Rac prophage; putative tail fiber assembly protein
RA 1,450,389 C→A 9.5% R70S (CGT→AGT)  paaD → ring 1,2‑phenylacetyl‑CoA epoxidase subunit
RA 1,460,689 A→G 9.2% pseudogene (1041/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,497,218 G→A 11.8% E169K (GAG→AAG)  ydcL → lipoprotein
RA 1,526,201 C→T 9.1% G43G (GGC→GGT ydcC → H repeat‑associated putative transposase
RA 1,558,535 C→A 11.8% D486Y (GAT→TAT)  dosP ← oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
RA 1,584,947 G→T 9.4% intergenic (‑154/+164) yneL ← / ← hipA pseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase
RA 1,593,201 C→A 11.4% S422S (TCG→TCT lsrK ← autoinducer‑2 (AI‑2) kinase
RA 1,607,385 C→A 10.8% G42W (GGG→TGG)  glsB ← glutaminase 2
RA 1,610,565 G→A 10.9% S182S (TCG→TCA yneK → uncharacterized protein
RA 1,628,897 G→C 10.2% G82G (GGC→GGG tfaQ ← Qin prophage; putative tail fiber assembly protein
RA 1,628,900 G→T 10.2% S81S (TCC→TCA tfaQ ← Qin prophage; putative tail fiber assembly protein
RA 1,628,903 A→C 10.0% A80A (GCT→GCG tfaQ ← Qin prophage; putative tail fiber assembly protein
RA 1,628,906 C→T 10.1% V79V (GTG→GTA tfaQ ← Qin prophage; putative tail fiber assembly protein
RA 1,643,508 G→T 22.7% intergenic (+210/‑131) ydfC → / → dicF uncharacterized protein, Qin prophage/sRNA antisense regulator, inhibits ftsZ, Hfq‑dependent; Qin prophage
RA 1,675,131 G→T 12.2% E440* (GAA→TAA)  ydgI → putative arginine/ornithine antiporter transporter
RA 1,703,236 C→T 13.8% V687V (GTC→GTT rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,242 G→A 14.7% A689A (GCG→GCA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,248 T→C 15.0% I691I (ATT→ATC rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,251 A→C 15.6% A692A (GCA→GCC rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,263 A→G 14.4% A696A (GCA→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,724,769 G→T 14.3% A476S (GCA→TCA)  lhr → putative ATP‑dependent helicase
RA 1,735,109 C→A 11.8% Y314* (TAC→TAA ydhC → putative arabinose efflux transporter
RA 1,736,203 G→T 23.5% W178C (TGG→TGT cfa → cyclopropane fatty acyl phospholipid synthase (unsaturated‑phospholipid methyltransferase)
RA 1,748,408 G→T 10.0% G109G (GGC→GGA ydhY ← putative 4Fe‑4S ferridoxin‑type protein; FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
RA 1,749,771 C→T 11.9% intergenic (‑373/‑184) ydhZ ← / → pykF uncharacterized protein/pyruvate kinase I
RA 1,754,778 C→A 18.3% M1M (ATG→ATT) †
*424L (TGA→TTA) 
sufS ←
sufD ←
cysteine desulfurase, stimulated by SufE; selenocysteine lyase, PLP‑dependent
component of SufBCD Fe‑S cluster assembly scaffold
RA 1,775,148 C→A 10.0% T164K (ACA→AAA)  ydiR → putative electron transfer flavoprotein, FAD‑binding subunit
RA 1,794,482 C→T 12.2% Q138Q (CAG→CAA infC ← translation initiation factor IF‑3
RA 1,810,480 C→T 12.2% intergenic (+95/+163) katE → / ← chbG catalase HPII, heme d‑containing/chito‑oligosaccharide deacetylase
RA 1,810,481 T→C 11.2% intergenic (+96/+162) katE → / ← chbG catalase HPII, heme d‑containing/chito‑oligosaccharide deacetylase
RA 1,810,486 A→T 10.8% intergenic (+101/+157) katE → / ← chbG catalase HPII, heme d‑containing/chito‑oligosaccharide deacetylase
RA 1,810,496 T→G 12.2% intergenic (+111/+147) katE → / ← chbG catalase HPII, heme d‑containing/chito‑oligosaccharide deacetylase
RA 1,861,011 T→C 11.8% intergenic (‑282/‑154) mipA ← / → yeaG scaffolding protein for murein synthesizing machinery/protein kinase, endogenous substrate unidentified; autokinase
RA 1,909,075 C→T 14.3% intergenic (‑2/+18) prc ← / ← proQ carboxy‑terminal protease for penicillin‑binding protein 3/RNA chaperone, putative ProP translation regulator
RA 1,910,770 G→T 27.2% E86* (GAA→TAA)  yebS → inner membrane protein
RA 1,947,767 G→T 33.3% L23L (CTG→CTT cmoB → tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent
RA 1,979,001 C→A 12.1% P203P (CCG→CCT araF ← L‑arabinose transporter subunit
RA 2,014,379 G→T 12.1% R271L (CGC→CTC)  fliM → flagellar motor switching and energizing component
RA 2,041,148 Δ1 bp 6.0% coding (2730/7077 nt) yeeJ → putative adhesin
RA 2,041,529 G→T 19.1% A1037A (GCG→GCT yeeJ → putative adhesin
RA 2,066,701 C→A 14.8% A561D (GCC→GAC)  flu → CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,085,564 C→A 15.4% I329I (ATC→ATA hisD → bifunctional histidinal dehydrogenase/ histidinol dehydrogenase
RA 2,142,070 G→T 8.9% G306C (GGT→TGT)  yegD → Hsp70 chaperone family protein
RA 2,201,725:1 +C 7.8% coding (280/1137 nt) yehP → VMA domain putative YehL ATPase stimulator
RA 2,201,725:2 +A 7.8% coding (280/1137 nt) yehP → VMA domain putative YehL ATPase stimulator
RA 2,216,086 G→T 9.1% V141V (GTG→GTT dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent
RA 2,226,252 C→A 12.1% intergenic (+45/‑105) cdd → / → sanA cytidine/deoxycytidine deaminase/vancomycin high temperature exclusion protein, DUF218 superfamily protein
RA 2,274,636 G→T 14.8% A176S (GCC→TCC)  yejH → putative ATP‑dependent DNA or RNA helicase
RA 2,328,265 C→T 5.9% L58L (CTG→CTA yfaT ← DUF1175 family protein, putative host defense protein
RA 2,375,355 C→A 10.3% T90K (ACG→AAG)  rbn → RNase BN, tRNA processing enzyme
RA 2,405,447 G→T 10.3% A41D (GCT→GAT)  yfbT ← sugar phosphatas
RA 2,409,887 C→A 11.5% D554E (GAC→GAA pta → phosphate acetyltransferase
RA 2,433,444 C→A 10.5% M52I (ATG→ATT yfcJ ← putative arabinose efflux transporter
RA 2,458,412 C→T 7.4% Q25Q (CAG→CAA mlaA ← ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
RA 2,504,418 T→C 10.6% intergenic (+53/‑62) yfeO → / → ypeC putative ion channel protein/DUF2502 family putative periplasmic protein
RA 2,504,420 C→G 10.3% intergenic (+55/‑60) yfeO → / → ypeC putative ion channel protein/DUF2502 family putative periplasmic protein
RA 2,504,422 T→G 10.5% intergenic (+57/‑58) yfeO → / → ypeC putative ion channel protein/DUF2502 family putative periplasmic protein
RA 2,510,465 G→A 7.4% Q283* (CAA→TAA)  yfeA ← putative diguanylate cyclase
RA 2,515,234 C→A 8.5% M201I (ATG→ATT xapR ← transcriptional activator of xapAB
RA 2,516,885 G→T 15.4% H154N (CAT→AAT)  xapB ← xanthosine transporter
RA 2,525,552 G→T 12.1% C244F (TGC→TTC)  cysZ → EI24 family inner membrane protein; putative sulfate transporter
RA 2,591,752 C→T 12.5% R158H (CGT→CAT)  bamC ← BamABCDE complex OM biogenesis lipoprotein
RA 2,619,717 C→A 14.8% A415E (GCG→GAG)  ppx → exopolyphosphatase
RA 2,625,889 C→A 8.2% T2T (ACG→ACT guaA ← GMP synthetase (glutamine aminotransferase)
RA 2,636,690 G→A 7.1% P318L (CCG→CTG)  rlmN ← dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent
RA 2,638,476 G→T 17.4% R737S (CGC→AGC)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain
RA 2,649,844 G→A 25.0% intergenic (‑127/+51) pepB ← / ← iscX aminopeptidase B/Iron binding protein associated with IscS; putative molecular adaptor of IscS function
RA 2,650,675 C→A 14.9% L540L (CTG→CTT hscA ← DnaK‑like molecular chaperone specific for IscU
RA 2,691,032 G→T 8.7% A62D (GCC→GAC)  tadA ← tRNA‑specific adenosine deaminase
RA 2,692,360 C→A 10.8% P81P (CCC→CCA yfhH → putative DNA‑binding transcriptional regulator
RA 2,707,696 G→A 6.9% intergenic (+107/+102) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,707,700 G→A 7.0% intergenic (+111/+98) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,707,703 C→T 8.8% intergenic (+114/+95) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,719,536 G→T 26.4% noncoding (12/120 nt) rrfG ← 5S ribosomal RNA of rrnG operon
RA 2,725,220 A→G 17.4% I771I (ATT→ATC clpB ← protein disaggregation chaperone
RA 2,761,401 A→G 13.6% Q111Q (CAA→CAG yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,761,407 C→T 12.5% P113P (CCC→CCT yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,761,410 G→C 12.5% R114R (CGG→CGC yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,809,576 C→T 15.1% D75N (GAT→AAT)  gshA ← glutamate‑cysteine ligase
RA 2,855,112 G→T 6.2% A108A (GCG→GCT ygbJ → putative dehydrogenase
RA 2,878,397 G→T 13.6% R728R (CGG→AGG)  ygcB ← R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity
RA 2,892,047 C→T 5.7% G244D (GGC→GAC)  ygcU ← putative FAD‑containing dehydrogenase
RA 2,941,193 G→T 15.9% Q393K (CAG→AAG)  amiC ← N‑acetylmuramoyl‑L‑alanine amidase
RA 2,955,920 G→A 11.3% N68N (AAC→AAT) ‡ ppdC ← putative prepilin peptidase‑dependent protein
RA 2,955,921 T→C 11.3% N68S (AAC→AGC) ‡ ppdC ← putative prepilin peptidase‑dependent protein
RA 2,955,926:1 +G 11.3% coding (198/324 nt) ppdC ← putative prepilin peptidase‑dependent protein
RA 2,964,345 G→T 16.7% A189A (GCG→GCT ygdQ → UPF0053 family inner membrane protein
RA 2,992,511 C→A 5.9% S247I (AGC→ATC)  ygeR ← LysM domain‑containing M23 family putative peptidase; septation lipoprotein
RA 3,012,924 G→T 12.5% S135S (TCG→TCT ssnA → putative chlorohydrolase/aminohydrolase
RA 3,013,960 A→G 6.6% Y21C (TAC→TGC)  ygfM → putative oxidoreductase
RA 3,017,494 C→A 20.6% R940R (CGC→CGA xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
RA 3,083,407 G→T 8.3% E98D (GAG→GAT yggI → Zn‑dependent metalloprotease‑related protein
RA 3,091,681 C→A 19.3% T352T (ACC→ACA yggW → HemN family putative oxidoreductase
RA 3,096,521 C→A 8.2% P50P (CCC→CCA mutY → adenine DNA glycosylase
RA 3,102,112 C→A 6.9% G135* (GGA→TGA)  speC ← ornithine decarboxylase, constitutive
RA 3,126,947 C→A 11.5% D124Y (GAC→TAC)  yghS ← putative ATP‑binding protein
RA 3,128,932 C→T 9.7% V267I (GTT→ATT)  pitB ← phosphate transporter
RA 3,136,579 G→A 6.7% F315F (TTC→TTT hybB ← putative hydrogenase 2 cytochrome b type component
RA 3,173,666 G→A 9.1% P188P (CCG→CCA ygiB → DUF1190 family protein
RA 3,184,204 G→T 7.9% G72C (GGC→TGC)  yqiI → fimbrial protein
RA 3,187,945 G→A 14.3% intergenic (+61/+137) yqiK → / ← sibD PHB family membrane protein, function unknown/sRNA antisense regulator of toxic IbsD protein
RA 3,236,263 C→A 8.7% A137A (GCG→GCT uxaA ← altronate hydrolase
RA 3,267,151 A→G 11.1% S372S (TCT→TCC garP ← putative (D)‑galactarate transporter
RA 3,290,110 C→A 12.1% Q115K (CAG→AAG)  yraP → outer membrane lipoprotein
RA 3,368,659 C→A 22.2% T144T (ACC→ACA dcuD → putative transporter
RA 3,408,568 T→C 9.1% D59D (GAT→GAC acrF → multidrug efflux system protein
RA 3,408,577 G→A 11.8% T62T (ACG→ACA acrF → multidrug efflux system protein
RA 3,416,899 A→G 14.8% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,420,206 G→A 11.9% intergenic (‑64/+111) rrlD ← / ← alaU 23S ribosomal RNA of rrnD operon/tRNA‑Ala
RA 3,421,849 T→A 11.0% noncoding (273/1542 nt) rrsD ← 16S ribosomal RNA of rrnD operon
RA 3,464,590 G→A 7.4% T33T (ACC→ACT tufA ← translation elongation factor EF‑Tu 1
RA 3,474,724 C→A 9.1% P26H (CCT→CAT)  yheS → putative transporter subunit of ABC superfamily: ATP‑binding component
RA 3,480,804 C→T 13.3% H219Y (CAT→TAT)  yhfK → putative transporter, FUSC superfamily inner membrane protein, tandem domains
RA 3,543,152 G→T 10.8% T93K (ACG→AAG)  malQ ← 4‑alpha‑glucanotransferase (amylomaltase)
RA 3,554,484 G→T 12.1% A110D (GCC→GAC)  glpG ← rhomboid intramembrane serine protease
RA 3,581,907 C→A 13.6% V212F (GTT→TTT)  ugpC ← glycerol‑3‑phosphate transporter subunit
RA 3,612,677 A→G 17.4% G42G (GGA→GGG rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,617,887 T→C 7.7% F50F (TTT→TTC yhhI → putative transposase
RA 3,628,084 C→T 28.6% intergenic (‑266/‑117) yhiL ← / → yhiM pseudogene, DUF4049 family protein/acid resistance protein, inner membrane
RA 3,632,609 A→C 11.8% intergenic (+108/+136) pitA → / ← uspB phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B
RA 3,633,367 G→T 12.5% intergenic (‑287/‑104) uspB ← / → uspA universal stress (ethanol tolerance) protein B/universal stress global response regulator
RA 3,686,493 C→A 8.9% E702* (GAG→TAG)  bcsA ← cellulose synthase, catalytic subunit
RA 3,799,878 C→A 6.5% E184* (GAA→TAA)  waaG ← glucosyltransferase I
RA 3,881,049 C→A 15.4% R288S (CGC→AGC)  mnmE → tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
RA 3,903,416 C→T 9.7% A115T (GCC→ACC)  pstC ← phosphate transporter subunit
RA 3,914,452 C→A 9.3% G33V (GGT→GTT)  atpE ← F0 sector of membrane‑bound ATP synthase, subunit c
RA 3,956,966 G→T 10.1% C208* (TGC→TGA gpp ← guanosine pentaphosphatase/exopolyphosphatase
RA 3,970,239 C→A 10.6% P201Q (CCG→CAG)  wzxE → O‑antigen translocase
RA 3,997,182 A→G 17.0% I119I (ATT→ATC) ‡ rarD ← putative chloramphenical resistance permease
RA 3,997,183 A→T 17.4% I119N (ATT→AAT) ‡ rarD ← putative chloramphenical resistance permease
RA 3,997,184 T→G 20.7% I119L (ATT→CTT) ‡ rarD ← putative chloramphenical resistance permease
RA 4,021,107 A→G 7.7% G342G (GGT→GGC fadA ← 3‑ketoacyl‑CoA thiolase (thiolase I)
RA 4,043,994 G→T 14.5% N44K (AAC→AAA yihA ← cell division GTP‑binding protein
RA 4,044,698 C→A 10.2% intergenic (+58/‑9) csrC → / → yihI CsrC sRNA sequesters CsrA, a carbon flux regulator/activator of Der GTPase
RA 4,053,474 G→T 6.6% E570* (GAG→TAG)  typA → GTP‑binding protein
RA 4,062,048 C→A 9.9% V197F (GTT→TTT)  yihQ ← alpha‑glucosidase
RA 4,091,237 T→C 8.7% L167P (CTG→CCG)  sodA → superoxide dismutase, Mn
RA 4,103,974 C→T 9.7% A143T (GCA→ACA)  fpr ← ferredoxin‑NADP reductase
RA 4,105,207 G→T 7.2% L101M (CTG→ATG)  glpX ← fructose 1,6‑bisphosphatase II
RA 4,120,246 G→T 5.6% A162S (GCC→TCC)  metL → Bifunctional aspartokinase/homoserine dehydrogenase 2
RA 4,151,990 C→A 6.9% L98I (CTC→ATC)  yijD → DUF1422 family inner membrane protein
RA 4,165,449 C→T 21.3% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 21.3% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,178,500 C→T 54.9% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,184,351 C→T 12.9% P559P (CCG→CCA thiC ← phosphomethylpyrimidine synthase
RA 4,216,666 Δ1 bp 11.1% coding (2911/3684 nt) metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
RA 4,216,669 G→A 10.2% E972K (GAG→AAG) ‡ metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
RA 4,216,670 A→C 11.8% E972A (GAG→GCG) ‡ metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
RA 4,249,947 G→T 15.2% intergenic (‑16/‑302) zur ← / → yjbL transcriptional repressor, Zn(II)‑binding/uncharacterized protein
RA 4,257,404 A→G 8.4% E121E (GAA→GAG tyrB → tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
RA 4,285,970 C→T 30.4% intergenic (+248/+283) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,316,156 T→G 18.4% intergenic (‑598/+60) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,316,157 C→T 16.9% intergenic (‑599/+59) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,324,099 T→C 8.0% N437D (AAT→GAT)  eptA ← lipid A phosphoethanolamine transferase
RA 4,332,586 C→A 11.4% R287S (CGT→AGT)  melA → alpha‑galactosidase, NAD(P)‑binding
RA 4,336,205 G→A 9.4% G313G (GGC→GGT fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,238 A→G 13.3% R302R (CGT→CGC fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,241 G→A 13.8% I301I (ATC→ATT fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,244 A→C 13.5% V300V (GTT→GTG fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,250 A→G 14.8% I298I (ATT→ATC fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,259 C→A 9.6% A295A (GCG→GCT fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,274 G→A 11.9% G290G (GGC→GGT fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,391,049 G→A 9.3% Q187Q (CAG→CAA hflX → GTPase, stimulated by 50S subunit binding
RA 4,434,547 C→T 10.3% P207S (CCG→TCG)  tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,436,184 C→A 8.2% I752I (ATC→ATA tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,498,944 G→A 10.9% pseudogene (119/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,498,951 C→A 6.4% pseudogene (126/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,501,840 T→A 7.3% K131M (AAG→ATG)  fecD ← iron‑dicitrate transporter subunit
RA 4,508,819 G→C 7.2% pseudogene (282/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,826 G→A 6.2% pseudogene (289/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,830 A→G 6.2% pseudogene (293/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,885 T→A 5.7% pseudogene (348/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,513,196 G→A 5.8% A239V (GCC→GTC)  yjhG ← putative dehydratase
RA 4,541,803 C→T 6.7% Y117Y (TAC→TAT uxuA → mannonate hydrolase
RA 4,575,004 Δ1 bp 8.3% pseudogene (1629/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,575,005 Δ1 bp 8.3% pseudogene (1628/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,575,006 Δ1 bp 8.3% pseudogene (1627/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,575,007 Δ1 bp 8.3% pseudogene (1626/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,575,008 Δ1 bp 8.3% pseudogene (1625/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,575,009 Δ1 bp 8.3% pseudogene (1624/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,575,010 Δ1 bp 8.3% pseudogene (1623/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,608,351 C→A 8.7% V102V (GTC→GTA deoA → thymidine phosphorylase
RA 4,608,500 C→T 10.0% A152V (GCG→GTG)  deoA → thymidine phosphorylase
RA 4,621,024 G→A 7.1% G159S (GGC→AGC)  slt → lytic murein transglycosylase, soluble

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 1310589 1310710 122 3 [1] [2] 3 [trpA] [trpA]
* * ÷ CP009273 1540360 1540420 61 3 [2] [2] 3 yddL/yddG putative lipoprotein;putative membrane; Not classified; putative outer membrane porin protein/aromatic amino acid exporter
* * ÷ CP009273 2050918 2050956 39 3 [1] [0] 3 yeeN UPF0082 family protein
* * ÷ CP009273 2463627 2463744 118 6 [2] [1] 4 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 3448799 3448836 38 4 [0] [0] 4 gspA/gspC general secretory pathway component, cryptic/general secretory pathway component, cryptic
* * ÷ CP009273 3745306 3745464 159 3 [1] [2] 3 [yiaU] [yiaU]
* * ÷ CP009273 3763048 3763143 96 3 [2] [1] 4 yibV pseudogene

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =11 (0.340)23 (0.710) 16/248 NT 67.6% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 12032619 (0.280)17 (0.610) 9/216 NT 81.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 0 (0.000)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related