Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	314,734	Δ1 bp	pseudogene (378/888 nt)	eaeH →	putative porin domain‑containing protein EaeH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	314,732	0	A	.	100.0%	108.9 / NA	24	pseudogene (376/888 nt)	eaeH	putative porin domain‑containing protein EaeH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/15);  total (9/15)

ACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCA  >  NC_000913/314657‑314807                                                                            |                                                                            acaGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATaagatt                                                                        >  2:61929/1‑77 (MQ=255)    gctaaagctaaCCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTtct                                                                    <  1:61897/81‑1 (MQ=255)        aagctaaCCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCg                                                                  <  1:61898/79‑1 (MQ=255)                   gAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGAttctt                                                     <  2:61899/81‑1 (MQ=255)                        aCAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTg                                                >  1:61933/1‑81 (MQ=255)                         cAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTgg                                               <  1:61900/81‑1 (MQ=255)                             aGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAAt                                           >  2:61934/1‑81 (MQ=255)                                  cTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCttt                                      <  2:61901/81‑1 (MQ=255)                                     gggAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTAt                                    <  1:61902/80‑1 (MQ=255)                                        aaaTATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGa                                >  2:61949/1‑81 (MQ=255)                                          atatGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGAtt                              <  2:61903/81‑1 (MQ=255)                                           tatGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGAttt                             <  2:61905/81‑1 (MQ=255)                                           tatGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGAttt                             <  1:61904/81‑1 (MQ=255)                                            atGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTa                            >  2:61941/1‑81 (MQ=255)                                             tGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTAt                           <  1:61906/81‑1 (MQ=255)                                             tGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTAt                           >  2:61935/1‑81 (MQ=255)                                               gTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATg                          <  2:61907/80‑1 (MQ=255)                                                tACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGAt                        <  2:61909/81‑1 (MQ=255)                                                tACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGAt                        <  1:61908/81‑1 (MQ=255)                                                     cgcgcgTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGc                    <  2:61910/80‑1 (MQ=255)                                                         cgTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGAc                >  2:61939/1‑80 (MQ=255)                                                             aaaCTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAat            <  2:61911/80‑1 (MQ=255)                                                                     tGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATgttgtt    >  1:61946/1‑80 (MQ=255)                                                                      gTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGATATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCa  >  2:61960/1‑81 (MQ=255)                                                                      gTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCa  >  1:61938/1‑81 (MQ=255)                                                                            |                                                                            ACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCA  >  NC_000913/314657‑314807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑