Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,177	(T)6→5	coding (358/699 nt)	proQ ←	RNA chaperone ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,172	0	T	.	100.0%	197.6 / NA	43	coding (363/699 nt)	proQ	RNA chaperone ProQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (21/22);  total (21/22)

CGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTA  >  NC_000913/1915092‑1915248                                                                                 |                                                                             cggcgTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGt                                                                              <  1:342845/81‑1 (MQ=255)  ggcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtt                                                                             >  2:342889/1‑81 (MQ=255)     gtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttttt                                                                          >  1:342878/1‑81 (MQ=255)      tggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttc                                                                         <  1:342846/81‑2 (MQ=255)        gcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttcg                                                                        <  2:342847/80‑3 (MQ=255)          gtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCtt                                                                    >  1:342910/1‑81 (MQ=255)           tAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTg                                                                   <  2:342848/81‑1 (MQ=255)             gTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCt                                                                 <  1:342849/81‑1 (MQ=255)               cGGACGTGGCTTGCGTTCGCGGCGCTGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGt                                                               >  1:342898/1‑81 (MQ=255)                 gACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTc                                                             <  1:342850/81‑1 (MQ=255)                  aCGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCa                                                            <  2:342851/81‑1 (MQ=255)                   cGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAg                                                           <  1:342852/81‑1 (MQ=255)                      ggCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCAc                                                        >  2:342900/1‑81 (MQ=255)                       gCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCAc                                                        <  1:342853/80‑1 (MQ=255)                       gCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCAc                                                        <  2:342854/80‑1 (MQ=255)                          tGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCtg                                                    >  2:342894/1‑81 (MQ=255)                          tGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCtg                                                    >  1:342888/1‑81 (MQ=255)                          tGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCtg                                                    >  1:342906/1‑81 (MQ=255)                           gCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCt                                                     <  2:342855/79‑1 (MQ=255)                           gCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCt                                                     <  1:342856/79‑1 (MQ=255)                                 gcggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                                              >  1:342902/1‑80 (MQ=255)                                 gcggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                                              <  1:342857/80‑1 (MQ=255)                                   ggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGaa                                            >  2:342899/1‑80 (MQ=255)                                   ggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGaa                                            >  1:342915/1‑80 (MQ=255)                                      gcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcg                                        >  1:342901/1‑81 (MQ=255)                                        ggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcgcg                                      <  1:342858/81‑1 (MQ=255)                                         gTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACgcgc                                     >  1:342914/1‑81 (MQ=255)                                          tGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCt                                    >  2:342922/1‑81 (MQ=255)                                           gCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCtt                                   >  1:342904/1‑81 (MQ=255)                                            cGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCttt                                  >  1:342893/1‑81 (MQ=255)                                               cttcttTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGc                               >  1:342908/1‑81 (MQ=255)                                                  ctttctCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGctt                             <  2:342860/80‑1 (MQ=255)                                                  ctttctCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGct                              <  1:342859/79‑1 (MQ=255)                                                  ctttctCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGct                              <  2:342861/79‑1 (MQ=255)                                                      ctcACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCttcttc                         >  1:342917/1‑80 (MQ=255)                                                         aCCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCa                        <  2:342862/78‑1 (MQ=255)                                                         aCCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgc                     <  1:342863/81‑1 (MQ=255)                                                             gcagTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgctgct                 <  1:342864/81‑1 (MQ=255)                                                              cagTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCtt                <  2:342865/81‑1 (MQ=255)                                                              cagTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCtt                >  1:342931/1‑81 (MQ=255)                                                                gTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGc              <  2:342866/81‑1 (MQ=255)                                                                  tGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGc              >  2:342920/1‑79 (MQ=255)                                                                   gCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGa            <  2:342867/80‑1 (MQ=255)                                                                   gCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGa            <  2:342868/80‑1 (MQ=255)                                                                    cGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAg           <  2:342869/80‑1 (MQ=255)                                                                        aGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGc      >  1:342903/1‑81 (MQ=255)                                                                          cTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGctc    >  1:342925/1‑81 (MQ=255)                                                                            tCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTa  >  1:342911/1‑81 (MQ=255)                                                                                 |                                                                             CGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTA  >  NC_000913/1915092‑1915248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑