Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,150,632	Δ1 bp	coding (354/1947 nt)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,150,629	0	A	.	100.0%	87.8 / NA	20	coding (357/1947 nt)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/10);  total (10/10)

TACGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGAAAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTA  >  NC_000913/2150554‑2150701                                                                            |                                                                         tACGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGaaatcc                                                                     >  1:383634/1‑78 (MQ=255) tACGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGaaatc                                                                      <  1:383610/80‑3 (MQ=255)       aCCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGca                                                              >  2:383635/1‑81 (MQ=255)            cgacgTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATg                                                         <  1:383611/81‑1 (MQ=255)                      gTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCTCACGCACAATGAGGGTAActc                                               >  2:383645/1‑81 (MQ=255)                      gTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAActc                                               >  1:383636/1‑81 (MQ=255)                              gAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATTAGGGTAACTCTGGcgac                                        >  1:383644/1‑80 (MQ=255)                                            gATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCtat                         <  2:383612/81‑1 (MQ=255)                                              tGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAg                       <  2:383613/81‑1 (MQ=255)                                                aaGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCta                          >  2:383643/1‑76 (MQ=255)                                                     atatTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCatat                >  2:383631/1‑81 (MQ=255)                                                      tatTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCatat                >  1:383647/1‑80 (MQ=255)                                                       atTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATg               >  1:383648/1‑80 (MQ=255)                                                        ttGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGaa             <  2:383614/81‑1 (MQ=255)                                                        ttGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGaa             >  2:383641/1‑81 (MQ=255)                                                          gcgcgcAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAAt            <  2:383615/80‑1 (MQ=255)                                                           cgcgcAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATc           <  1:383617/80‑1 (MQ=255)                                                           cgcgcAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCg          <  2:383616/81‑1 (MQ=255)                                                              gcAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGtt       >  2:383632/1‑81 (MQ=255)                                                                aaCATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCt     <  2:383619/81‑1 (MQ=255)                                                                aaCATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCt     <  1:383618/81‑1 (MQ=255)                                                                   aTAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTa  <  1:383620/81‑1 (MQ=255)                                                                            |                                                                         TACGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGAAAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTA  >  NC_000913/2150554‑2150701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑