Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,797,159	(A)6→5	coding (212/1260 nt)	waaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,797,154	0	A	.	100.0%	12.2 / NA	4	coding (207/1260 nt)	waaL	O‑antigen ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (3/1);  total (3/1)

TAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAA  >  NC_000913/3797088‑3797206                                                                   |                                                     taataTCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTAttctt                                       >  2:701919/1‑81 (MQ=255)   atatGACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTAttcttc                                      <  1:701910/80‑1 (MQ=255)                        tATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAgg                >  2:701923/1‑81 (MQ=255)                                      ttACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTaa  >  1:701926/1‑81 (MQ=255)                                                                   |                                                     TAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAA  >  NC_000913/3797088‑3797206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑