Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,949,783	(T)7→6	coding (187/1521 nt)	yifB ←	putative magnesium chelatase YifB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,949,777	0	T	.	100.0%	103.3 / NA	23	coding (193/1521 nt)	yifB	putative magnesium chelatase YifB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/11);  total (12/11)

GCCAGCAACGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGATTTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTACCGT  >  NC_000913/3949698‑3949849                                                                                |                                                                         gccAGCAACGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGAtt                                                                         >  1:732531/1‑81 (MQ=255)    aGCAACGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGAttttt                                                                      <  1:732489/81‑1 (MQ=255)      cAACGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGAttttttc                                                                    <  2:732490/81‑2 (MQ=255)         cGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGAttttttcgcc                                                                 <  2:732491/81‑5 (MQ=255)             aTGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGat                                                            <  2:732492/81‑1 (MQ=255)               ggCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGatat                                                          >  1:732524/1‑81 (MQ=255)                   aTAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCat                                                      <  2:732493/81‑1 (MQ=255)                      ggTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCatat                                                    <  1:732494/80‑1 (MQ=255)                      ggTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATc                                                   >  2:732517/1‑81 (MQ=255)                       gTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATcc                                                  <  1:732495/81‑1 (MQ=255)                          aaTCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCt                                               <  1:732496/81‑1 (MQ=255)                              atatCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTg                                           <  1:732497/81‑1 (MQ=255)                                     cccccTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATgg                                    >  2:732520/1‑81 (MQ=255)                                           tcttTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTgc                              >  1:732530/1‑81 (MQ=255)                                                tGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCAc                          <  2:732498/80‑1 (MQ=255)                                                  gCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCAcgc                        <  2:732499/80‑1 (MQ=255)                                                     gATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATc                    >  1:732540/1‑81 (MQ=255)                                                     gATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATc                    <  2:732500/81‑1 (MQ=255)                                                      aTCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATcg                   >  2:732538/1‑81 (MQ=255)                                                         aGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATcgc                  >  1:732543/1‑79 (MQ=255)                                                         aGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATcgc                  >  1:732529/1‑79 (MQ=255)                                                           cTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGc              >  2:732535/1‑81 (MQ=255)                                                             ggAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGctt            >  2:732522/1‑81 (MQ=255)                                                              gAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGcttc           >  1:732523/1‑81 (MQ=255)                                                                   aGGTTGATGGAGA‑TTTTCTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGAGAGCTTCTTTTa      >  2:732526/1‑81 (MQ=255)                                                                    ggTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTAc     <  1:732501/81‑1 (MQ=255)                                                                        gATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTACCgt  <  1:732502/80‑1 (MQ=255)                                                                                |                                                                         GCCAGCAACGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGATTTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTACCGT  >  NC_000913/3949698‑3949849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑