Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	304,754	C→T	R151Q (CGA→CAA)	ecpE ←	putative fimbrial chaperone EcpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	304,754	0	C	T	100.0%	110.6 / NA	29	R151Q (CGA→CAA)	ecpE	putative fimbrial chaperone EcpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/15);  total (14/15)

AACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAT  >  NC_000913/304679‑304829                                                                            |                                                                            aaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtact                                                                        <  1:60150/81‑1 (MQ=255) aaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtac                                                                         >  2:60182/1‑80 (MQ=255)  aCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtacta                                                                       <  1:60151/81‑1 (MQ=255)   ccATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtactac                                                                      >  2:60198/1‑81 (MQ=255)          ttGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATg                                                               >  2:60179/1‑81 (MQ=255)            gCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATgg                                                              >  2:60194/1‑80 (MQ=255)                   ttacttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCAt                                                       <  2:60152/80‑1 (MQ=255)                        tacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcaccac                                                 >  2:60196/1‑81 (MQ=255)                           cGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcaccaccac                                              >  2:60197/1‑81 (MQ=255)                             ttCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCg                                            <  2:60153/81‑1 (MQ=255)                               ccTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCgg                                           <  2:60154/80‑1 (MQ=255)                               ccTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGc                                          >  2:60184/1‑81 (MQ=255)                               ccTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGc                                          >  2:60180/1‑81 (MQ=255)                                  gTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTcc                                       >  2:60190/1‑81 (MQ=255)                                     aCCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGt                                     <  2:60155/80‑1 (MQ=255)                                          gTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGAc                               >  2:60200/1‑81 (MQ=255)                                             gAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAc                            <  2:60156/81‑1 (MQ=255)                                             gAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAc                            >  1:60187/1‑81 (MQ=255)                                              aaGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcg                           >  1:60210/1‑81 (MQ=255)                                               aGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgc                          <  1:60157/81‑1 (MQ=255)                                                ggACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgcc                         <  1:60158/81‑1 (MQ=255)                                                 gACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgccg                        <  2:60159/81‑1 (MQ=255)                                                        tAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTg                 >  1:60206/1‑81 (MQ=255)                                                           aCTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGc              <  1:60160/81‑1 (MQ=255)                                                                aCCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGt         <  2:60161/81‑1 (MQ=255)                                                                aCCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGt         <  2:60162/81‑1 (MQ=255)                                                                     aCGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTg     >  1:60207/1‑80 (MQ=255)                                                                     aCGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGt    <  1:60163/81‑1 (MQ=255)                                                                       gTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAt  <  2:60164/81‑1 (MQ=255)                                                                            |                                                                            AACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAT  >  NC_000913/304679‑304829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑