Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	311,801	G→A	intergenic (‑465/‑311)	ecpR ← / → ykgL	DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	311,801	0	G	A	100.0%	102.2 / NA	27	intergenic (‑465/‑311)	ecpR/ykgL	DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/10);  total (17/10)

TAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCG  >  NC_000913/311727‑311877                                                                           |                                                                             tAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGcac                                                                        <  2:61522/81‑1 (MQ=255) tAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGcac                                                                        <  2:61521/81‑1 (MQ=255)       ataAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGCACAAAATg                                                                  >  2:61541/1‑81 (MQ=255)           aaCTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTa                                                              >  1:61540/1‑81 (MQ=255)             cTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTa                                                              >  1:61543/1‑79 (MQ=255)                 gatgaGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTACAGgtg                                                        >  1:61554/1‑81 (MQ=255)                          tCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTcc                                                >  2:61542/1‑80 (MQ=255)                           cATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCaa                                              >  1:61544/1‑81 (MQ=255)                            aTTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAAt                                             <  2:61523/81‑1 (MQ=255)                               gAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAg                                          >  2:61547/1‑81 (MQ=255)                                    tgatTTGCCAATCAGTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATg                                     <  1:61524/81‑1 (MQ=255)                                             aaTCAGTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTg                             >  2:61553/1‑80 (MQ=255)                                                 aGTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACg                         <  1:61525/80‑1 (MQ=255)                                                  gTAAGATATTTTAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACggg                       >  1:61546/1‑81 (MQ=255)                                                    aaGATATTTTAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCg                     >  1:61565/1‑81 (MQ=255)                                                            ttAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTAc              <  2:61526/80‑1 (MQ=255)                                                            ttAATGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACg             >  1:61545/1‑81 (MQ=255)                                                                tGGATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCAt         >  1:61564/1‑81 (MQ=255)                                                                  gATTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATc        <  2:61527/80‑1 (MQ=255)                                                                   aTTAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTg      <  2:61528/81‑1 (MQ=255)                                                                    ttAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGt     >  1:61549/1‑81 (MQ=255)                                                                    ttAACATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGt     >  2:61555/1‑81 (MQ=255)                                                                      aaCATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGtt    <  2:61529/80‑1 (MQ=255)                                                                      aaCATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTc   >  1:61551/1‑81 (MQ=255)                                                                       aCATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTc   <  1:61530/80‑1 (MQ=255)                                                                       aCATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCg  >  1:61548/1‑81 (MQ=255)                                                                       aCATACCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCg  >  2:61550/1‑81 (MQ=255)                                                                           |                                                                             TAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCG  >  NC_000913/311727‑311877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑