Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	334,105	T→G	H110P (CAC→CCC)	yahB ←	putative LysR‑type DNA‑binding transcriptional regulator YahB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	334,105	0	T	G	100.0%	85.1 / NA	24	H110P (CAC→CCC)	yahB	putative LysR‑type DNA‑binding transcriptional regulator YahB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/15);  total (9/15)

TACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCT  >  NC_000913/334033‑334181                                                                         |                                                                             taCACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGc                                                                      <  2:65226/81‑1 (MQ=255)  acacTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCg                                                                     >  2:65274/1‑81 (MQ=255)   cacTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGt                                                                    <  1:65227/81‑1 (MQ=255)     cTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGtat                                                                  <  2:65229/81‑1 (MQ=255)     cTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGtat                                                                  <  2:65228/81‑1 (MQ=255)     cTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGta                                                                   >  1:65266/1‑80 (MQ=255)          gtggtgACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAag                                                              >  2:65261/1‑80 (MQ=255)              tgACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCt                                                         <  2:65230/81‑1 (MQ=255)                    tAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTaa                                                   <  1:65231/81‑1 (MQ=255)                      aTCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATa                                                 >  1:65257/1‑81 (MQ=255)                      aTCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATa                                                 >  2:65258/1‑81 (MQ=255)                            cAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATg                                           >  2:65282/1‑81 (MQ=255)                                   gAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCacac                                    >  1:65265/1‑81 (MQ=255)                                   gAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCacac                                    <  2:65232/81‑1 (MQ=255)                                     aaCTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACAcgc                                  <  2:65233/81‑1 (MQ=255)                                          cTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTc                             <  2:65234/81‑1 (MQ=255)                                             tttCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTAt                          >  1:65280/1‑81 (MQ=255)                                                  gCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCt                     <  1:65236/81‑1 (MQ=255)                                                  gCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCt                     <  1:65235/81‑1 (MQ=255)                                                   cACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTg                    <  1:65237/81‑1 (MQ=255)                                                      cTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGa                 <  2:65238/81‑1 (MQ=255)                                                               gCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATc        <  1:65239/81‑1 (MQ=255)                                                                 gTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGa      >  2:65270/1‑81 (MQ=255)                                                                     ggTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCt  <  1:65240/81‑1 (MQ=255)                                                                         |                                                                             TACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCT  >  NC_000913/334033‑334181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑