Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	538,029	A→G	I133V (ATT→GTT)	ybbW →	putative allantoin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	538,029	0	A	G	100.0%	93.7 / NA	27	I133V (ATT→GTT)	ybbW	putative allantoin transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/15);  total (12/15)

CGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTA  >  NC_000913/537953‑538100                                                                             |                                                                        cGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCt                                                                     >  2:105221/1‑81 (MQ=255)  ggATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTg                                                                    <  1:105192/81‑1 (MQ=255)        ttaAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTgg                                                               >  1:105212/1‑80 (MQ=255)        ttaAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGc                                                              <  1:105193/81‑1 (MQ=255)            ggggCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCaaaa                                                          >  1:105218/1‑81 (MQ=255)             gggCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAAt                                                         <  1:105194/81‑1 (MQ=255)               gCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCt                                                       <  2:105195/81‑1 (MQ=255)                cGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTg                                                      >  2:105238/1‑81 (MQ=255)                  gAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGc                                                    <  2:105196/81‑1 (MQ=255)                   aaTTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGcc                                                   <  1:105198/81‑1 (MQ=255)                   aaTTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGcc                                                   <  2:105197/81‑1 (MQ=255)                        ccgccATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAt                                              >  1:105219/1‑81 (MQ=255)                        ccgccATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAt                                              <  1:105199/81‑1 (MQ=255)                         cgccATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAtt                                             <  2:105200/81‑1 (MQ=255)                          gccATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAttt                                            >  1:105229/1‑81 (MQ=255)                                atGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAAct                                      >  2:105223/1‑81 (MQ=255)                                  gtgGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAActct                                    >  2:105220/1‑81 (MQ=255)                                    ggtttggtttGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCg                                  >  2:105233/1‑81 (MQ=255)                                         ggtttgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtgg                              <  1:105201/80‑1 (MQ=255)                                          gtttgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtggt                             <  2:105202/80‑1 (MQ=255)                                             tgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGAtt                         >  1:105234/1‑81 (MQ=255)                                              gCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGAtt                         <  2:105203/80‑1 (MQ=255)                                                         cgGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAg             >  1:105232/1‑81 (MQ=255)                                                           gggTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGc           <  2:105205/81‑1 (MQ=255)                                                           gggTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGc           <  1:105204/81‑1 (MQ=255)                                                            ggTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGcc          <  1:105206/81‑1 (MQ=255)                                                                    gCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTa  >  2:105236/1‑81 (MQ=255)                                                                             |                                                                        CGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTA  >  NC_000913/537953‑538100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑