Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	698,541	G→T	R213S (CGT→AGT)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	698,541	0	G	T	100.0%	79.3 / NA	22	R213S (CGT→AGT)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/9);  total (13/9)

CAGACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACG  >  NC_000913/698464‑698613                                                                              |                                                                         cAGACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCa                                                                        <  1:134502/80‑1 (MQ=255)  aGACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGc                                                                      >  2:134527/1‑81 (MQ=255)   gACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCt                                                                     <  1:134503/81‑1 (MQ=255)       accaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGtt                                                                 <  2:134504/81‑1 (MQ=255)                 agcaCACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCTGt                                                        >  2:134530/1‑80 (MQ=255)                 agcaCACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGt                                                        >  2:134532/1‑80 (MQ=255)                   cacaCCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCGGTGCCTGACTCAGCTCGTTTTTGTCGGTCAc                                                     <  2:134505/81‑1 (MQ=255)                        cGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTAt                                                <  2:134506/81‑1 (MQ=255)                              gCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCa                                          >  2:134556/1‑81 (MQ=255)                                acatcagacatcagATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCAcc                                        >  1:134549/1‑81 (MQ=255)                                  atcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCg                                       >  1:134553/1‑80 (MQ=255)                                        catcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCg                                  >  2:134539/1‑79 (MQ=255)                                        catcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTa                                <  1:134507/81‑1 (MQ=255)                                         atcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAg                               >  1:134533/1‑81 (MQ=255)                                          tcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGt                              <  1:134508/81‑1 (MQ=255)                                                 gCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAAcc                        <  2:134509/80‑1 (MQ=255)                                                     ttAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGt                     <  1:134510/79‑1 (MQ=255)                                                        aCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCga                >  2:134538/1‑81 (MQ=255)                                                              tCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAg          >  1:134544/1‑81 (MQ=255)                                                              tCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAg          >  2:134555/1‑81 (MQ=255)                                                                     gTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAAc   >  2:134545/1‑81 (MQ=255)                                                                       gCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACg  >  1:134537/1‑80 (MQ=255)                                                                              |                                                                         CAGACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACG  >  NC_000913/698464‑698613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑