Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	742,653	G→A	intergenic (‑97/‑174)	dtpD ← / → ybgI	dipeptide:H(+) symporter DtpD/radiation resistance protein YbgI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	742,653	0	G	A	100.0%	92.0 / NA	25	intergenic (‑97/‑174)	dtpD/ybgI	dipeptide:H(+) symporter DtpD/radiation resistance protein YbgI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/12);  total (13/12)

GCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCG  >  NC_000913/742576‑742729                                                                              |                                                                             gCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACaa                                                                           <  2:142437/81‑1 (MQ=255)    aCGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAatat                                                                        <  2:142438/81‑1 (MQ=255)    aCGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAatat                                                                        <  2:142439/81‑1 (MQ=255)      gTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGc                                                                      >  2:142458/1‑81 (MQ=255)          cGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTc                                                                  >  2:142463/1‑81 (MQ=255)           gTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCt                                                                 <  2:142440/81‑1 (MQ=255)              aTAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGAt                                                              >  1:142459/1‑81 (MQ=255)              aTAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGAt                                                              <  2:142441/81‑1 (MQ=255)                         gcgTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAatat                                                   <  2:142442/81‑1 (MQ=255)                            tGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACa                                                <  2:142443/81‑1 (MQ=255)                                   gtTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCt                                          >  2:142462/1‑80 (MQ=255)                                     ttCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTg                                         >  1:142456/1‑79 (MQ=255)                                           aaaGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGtga                                 >  2:142466/1‑81 (MQ=255)                                           aaaGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGtga                                 >  1:142470/1‑81 (MQ=255)                                           aaaGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGt                                   <  1:142444/79‑1 (MQ=255)                                            aaGTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGtgat                                <  1:142445/81‑1 (MQ=255)                                              gTTAAAACTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGtgatga                              >  1:142460/1‑81 (MQ=255)                                                   aaCTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGAtata                          >  2:142454/1‑80 (MQ=255)                                                     cTTTGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGATATaaa                        >  1:142457/1‑80 (MQ=255)                                                       ttGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACaa                     >  2:142467/1‑81 (MQ=255)                                                        tGATACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAAt                    >  1:142465/1‑81 (MQ=255)                                                          aTACAGATCTGATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAAttt                  <  1:142446/81‑1 (MQ=255)                                                                    gATTATTTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTg        >  1:142464/1‑81 (MQ=255)                                                                     attattTATACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTgg       <  1:142447/81‑1 (MQ=255)                                                                         tttatACAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTc   <  2:142448/81‑1 (MQ=255)                                                                           tataCAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCg  <  1:142449/80‑1 (MQ=255)                                                                              |                                                                             GCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCG  >  NC_000913/742576‑742729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑