Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	79,341	C→A	G38V (GGT→GTT)	leuD ←	3‑isopropylmalate dehydratase subunit LeuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	79,341	0	C	A	100.0%	112.6 / NA	31	G38V (GGT→GTT)	leuD	3‑isopropylmalate dehydratase subunit LeuD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/20);  total (11/20)

CAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAACCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGG  >  NC_000913/79265‑79414                                                                             |                                                                          aagaaCGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTAGTTAAACAGATGCGCGCAAAAAACCGt                                                                       <  2:16166/77‑1 (MQ=255)       gAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGt                                                                 >  1:16202/1‑81 (MQ=255)        aaGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTc                                                                <  1:16167/81‑1 (MQ=255)         aGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCa                                                               >  2:16211/1‑81 (MQ=255)             cGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACttt                                                           <  1:16168/81‑1 (MQ=255)               ggTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCt                                                         <  1:16169/81‑1 (MQ=255)               ggTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCt                                                         >  1:16201/1‑81 (MQ=255)                    ggCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCaa                                                     >  1:16196/1‑80 (MQ=255)                     gCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCaaa                                                    <  2:16170/80‑1 (MQ=255)                      cTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCaaaaa                                                  >  2:16203/1‑81 (MQ=255)                        gTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACt                                                >  2:16210/1‑81 (MQ=255)                         ttGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAAc                                                 <  1:16171/79‑1 (MQ=255)                           ggCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGt                                              <  2:16172/80‑1 (MQ=255)                             ccTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTc                                           <  1:16173/81‑1 (MQ=255)                                 tttCATCCAGAAAGCACCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGAGTCACTTTCTGCAAAAACTGTTTCggg                                        <  2:16174/80‑1 (MQ=255)                                 tttCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCggg                                        <  2:16175/80‑1 (MQ=255)                                 tttCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGga                                       >  2:16204/1‑81 (MQ=255)                                   tCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgat                                      <  2:16176/80‑1 (MQ=255)                                   tCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgat                                      >  1:16212/1‑80 (MQ=255)                                      tCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGAtt                                  >  1:16208/1‑81 (MQ=255)                                        cAGAAAACGCCAGTCGTTAAACAGGTGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGc                                <  1:16177/81‑1 (MQ=255)                                          gAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCAt                              >  2:16214/1‑81 (MQ=255)                                             aaCGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCgg                           <  1:16178/81‑1 (MQ=255)                                              aCGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCgg                           >  2:16207/1‑80 (MQ=255)                                                   aGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGa                     <  2:16179/81‑1 (MQ=255)                                                        ttAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTg                <  1:16181/81‑1 (MQ=255)                                                        ttAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTg                <  1:16180/81‑1 (MQ=255)                                                                 tGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATcc       <  2:16182/81‑1 (MQ=255)                                                                   cgcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCa      <  2:16183/80‑1 (MQ=255)                                                                     cgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCg   <  2:16184/81‑1 (MQ=255)                                                                      gcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCgg  <  1:16185/81‑1 (MQ=255)                                                                             |                                                                          CAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAACCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGG  >  NC_000913/79265‑79414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑