Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	797,736	T→C	E232E (GAA→GAG)	ybhA ←	pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	797,736	0	T	C	100.0%	108.7 / NA	30	E232E (GAA→GAG)	ybhA	pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/14);  total (16/14)

TTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACC  >  NC_000913/797659‑797811                                                                              |                                                                            ttATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTc                                                                           >  1:152780/1‑80 (MQ=255)        cAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATAc                                                                   <  1:152734/81‑1 (MQ=255)         aaTCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACt                                                                  <  2:152735/81‑1 (MQ=255)            cacaATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGa                                                                >  2:152785/1‑80 (MQ=255)            cacaATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGa                                                                >  1:152792/1‑80 (MQ=255)              caATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGatat                                                             <  2:152738/81‑1 (MQ=255)              caATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGatat                                                             <  2:152737/81‑1 (MQ=255)              caATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGatat                                                             <  2:152736/81‑1 (MQ=255)                 tGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCAt                                                          <  2:152739/81‑1 (MQ=255)                  gTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCAt                                                          >  2:152788/1‑80 (MQ=255)                   ttGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCAtt                                                         <  1:152740/80‑1 (MQ=255)                           cgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATc                                                <  1:152741/81‑1 (MQ=255)                                ttACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGcc                                             <  1:152742/79‑1 (MQ=255)                                ttACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCg                                            <  1:152743/80‑1 (MQ=255)                                   ccGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATg                                        >  2:152777/1‑81 (MQ=255)                                      cgTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCa                                     >  2:152799/1‑81 (MQ=255)                                            cGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGt                               >  2:152798/1‑81 (MQ=255)                                             ggCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGtt                              <  1:152744/81‑1 (MQ=255)                                              gCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGttt                             <  1:152745/81‑1 (MQ=255)                                                 ttGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTc                           >  1:152790/1‑80 (MQ=255)                                                   gCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCAt                        >  1:152787/1‑81 (MQ=255)                                                               gCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTg            >  1:152789/1‑81 (MQ=255)                                                                 cTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTgcg          <  1:152746/81‑1 (MQ=255)                                                                    tACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCt       >  1:152807/1‑81 (MQ=255)                                                                    tACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCt       <  1:152747/81‑1 (MQ=255)                                                                     aCCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCt       >  1:152782/1‑80 (MQ=255)                                                                     aCCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCt       >  2:152795/1‑80 (MQ=255)                                                                       cAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCaa    >  1:152797/1‑81 (MQ=255)                                                                        aGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAAc   >  2:152794/1‑81 (MQ=255)                                                                          cGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAAcc  >  2:152802/1‑80 (MQ=255)                                                                              |                                                                            TTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACC  >  NC_000913/797659‑797811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑