Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	919,229	A→G	intergenic (‑109/‑6)	ybjX ← / → macA	DUF535 domain‑containing protein YbjX/ABC‑type tripartite efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	919,229	0	A	G	100.0%	100.7 / NA	27	intergenic (‑109/‑6)	ybjX/macA	DUF535 domain‑containing protein YbjX/ABC‑type tripartite efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/8);  total (19/8)

TTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTAC  >  NC_000913/919152‑919305                                                                              |                                                                             ttACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAAt                                                                           >  1:174779/1‑81 (MQ=255)          tAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGaaaa                                                                  <  1:174754/81‑1 (MQ=255)                ttAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCgg                                                             >  2:174773/1‑80 (MQ=255)                  aGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGaa                                                           >  1:174782/1‑80 (MQ=255)                      ttttGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCg                                                      >  1:174787/1‑81 (MQ=255)                        ttgttgATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGt                                                     <  2:174755/80‑1 (MQ=255)                          gttgATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTga                                                   <  1:174756/80‑1 (MQ=255)                          gttgATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTg                                                    >  1:174788/1‑79 (MQ=255)                           ttgATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTg                                                    >  2:174792/1‑78 (MQ=255)                           ttgATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTg                                                    >  1:174783/1‑78 (MQ=255)                             gATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTgaag                                                 >  1:174766/1‑79 (MQ=255)                               tatTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGc                                             <  2:174757/81‑1 (MQ=255)                                  ttCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGcgt                                           >  1:174795/1‑80 (MQ=255)                                   tCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGcgtt                                          <  2:174758/80‑1 (MQ=255)                                            ttAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTgc                                >  1:174797/1‑81 (MQ=255)                                            ttAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTgc                                <  2:174759/81‑1 (MQ=255)                                             tAATGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTgcg                               >  2:174781/1‑81 (MQ=255)                                              aaTGACCCGGATTGGCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTgcgc                              >  2:174780/1‑81 (MQ=255)                                                           ggCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCAt                 >  1:174790/1‑81 (MQ=255)                                                           ggCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCAt                 >  1:174801/1‑81 (MQ=255)                                                           ggCATATGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCAt                 >  1:174784/1‑81 (MQ=255)                                                               tatGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGcc             <  1:174760/81‑1 (MQ=255)                                                                atGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCg            >  1:174778/1‑81 (MQ=255)                                                                atGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCg            >  1:174794/1‑81 (MQ=255)                                                                 tGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCgg           <  2:174761/81‑1 (MQ=255)                                                                 tGGAGTATTCAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCg            >  1:174764/1‑80 (MQ=255)                                                                          cAGAGAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTAc  >  1:174793/1‑81 (MQ=255)                                                                              |                                                                             TTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTAC  >  NC_000913/919152‑919305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑