Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	922,079	T→G	L578R (CTG→CGG)	macB →	ABC‑type tripartite efflux pump ATP binding/membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	922,079	0	T	G	100.0%	119.1 / NA	33	L578R (CTG→CGG)	macB	ABC‑type tripartite efflux pump ATP binding/membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/16);  total (17/16)

GCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTACCC  >  NC_000913/922002‑922155                                                                              |                                                                             gcgGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGt                                                                           >  2:175327/1‑81 (MQ=255)  cgGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGtt                                                                          >  2:175332/1‑81 (MQ=255)   gggAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGttt                                                                         <  1:175288/81‑1 (MQ=255)    ggAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTg                                                                        <  2:175289/81‑1 (MQ=255)                     tGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGt                                                       <  1:175290/81‑1 (MQ=255)                       gCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTg                                                     <  2:175292/81‑1 (MQ=255)                       gCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTg                                                     <  2:175291/81‑1 (MQ=255)                          gTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTggg                                                   >  2:175326/1‑80 (MQ=255)                           tAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTggg                                                   <  1:175293/79‑1 (MQ=255)                                gcgcGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAaca                                            >  2:175330/1‑81 (MQ=255)                                 cgcgAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAacac                                           >  2:175338/1‑81 (MQ=255)                                 cgcgAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAacac                                           <  2:175294/81‑1 (MQ=255)                                  gcgAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcact                                          >  1:175321/1‑81 (MQ=255)                                      gCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtca                                      >  1:175333/1‑81 (MQ=255)                                            gATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGtt                                 >  1:175324/1‑80 (MQ=255)                                            gATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGtt                                 >  1:175339/1‑80 (MQ=255)                                            gATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGtt                                 >  2:175331/1‑80 (MQ=255)                                             aTGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTaa                               <  1:175295/81‑1 (MQ=255)                                                ttttGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAAtt                             <  2:175296/80‑1 (MQ=255)                                                ttttGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTg                            <  1:175297/81‑1 (MQ=255)                                                    gCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCttt                        >  2:175329/1‑81 (MQ=255)                                                    gCAACAGTTCCTGATCGAAGCCGTACGGGTATGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCtt                         <  2:175298/80‑1 (MQ=255)                                                     cAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCttt                        >  2:175337/1‑80 (MQ=255)                                                      aaCAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCa                      <  2:175299/81‑1 (MQ=255)                                                       aCAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCAc                     <  1:175300/81‑1 (MQ=255)                                                         aGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCt                   >  1:175357/1‑81 (MQ=255)                                                         aGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCt                   >  2:175343/1‑81 (MQ=255)                                                           ttCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTg                 >  2:175328/1‑81 (MQ=255)                                                                    gAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCtttt         <  2:175301/80‑1 (MQ=255)                                                                    gAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTc        >  2:175336/1‑81 (MQ=255)                                                                    gAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTc        >  2:175340/1‑81 (MQ=255)                                                                        ccGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTAc    <  1:175302/81‑1 (MQ=255)                                                                          gTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTAccc  <  2:175303/81‑1 (MQ=255)                                                                              |                                                                             GCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTACCC  >  NC_000913/922002‑922155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑