Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,064,853	A→T	D273V (GAT→GTT)	yccE →	uncharacterized protein YccE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,064,853	0	A	T	100.0%	46.8 / NA	14	D273V (GAT→GTT)	yccE	uncharacterized protein YccE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/2);  total (12/2)

AGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTAAAA  >  NC_000913/1064790‑1064921                                                                |                                                                     aGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAAc                                                      >  2:200788/1‑80 (MQ=255)         gttgATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAg                                             >  1:200806/1‑81 (MQ=255)            gATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAgggg                                          >  2:200805/1‑81 (MQ=255)             aTACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAgggg                                          >  1:200811/1‑80 (MQ=255)             aTACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAgggg                                          >  1:200814/1‑80 (MQ=255)                    aTTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGagtag                                  >  2:200817/1‑81 (MQ=255)                       cTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCt                               >  2:200812/1‑81 (MQ=255)                          taATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTTAGGGGCAGAGTAGTCTTAt                            >  1:200815/1‑81 (MQ=255)                          taATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTAt                            <  2:200784/81‑1 (MQ=255)                           aaTCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATg                           >  2:200818/1‑81 (MQ=255)                                       gATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCttt               <  1:200785/81‑1 (MQ=255)                                       gATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCttt               >  1:200810/1‑81 (MQ=255)                                                aCGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGttttt       >  2:200807/1‑80 (MQ=255)                                                     aTTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTaaaa  >  2:200813/1‑80 (MQ=255)                                                                |                                                                     AGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTAAAA  >  NC_000913/1064790‑1064921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑