Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,096,234	C→G	P131R (CCG→CGG)	ycdU →	uncharacterized protein YcdU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,096,234	0	C	G	100.0%	89.4 / NA	25	P131R (CCG→CGG)	ycdU	uncharacterized protein YcdU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (15/10);  total (15/10)

TTGAGTGCAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCCGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGAAAAAAG  >  NC_000913/1096158‑1096311                                                                             |                                                                              ttGAGTGCAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTg                                                                           >  1:206242/1‑81 (MQ=255) ttGAGTGCAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTg                                                                           >  2:206235/1‑81 (MQ=255)               ttGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTctgct                                                             <  2:206204/81‑1 (MQ=255)               ttGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTctgct                                                             >  2:206233/1‑81 (MQ=255)                tGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTctgctg                                                            >  1:206240/1‑81 (MQ=255)                    ggCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTc                                                        >  1:206238/1‑81 (MQ=255)                    ggCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTc                                                        <  2:206205/81‑1 (MQ=255)                       tataCCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGga                                                     >  1:206234/1‑81 (MQ=255)                         taCCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGgat                                                    <  2:206206/80‑1 (MQ=255)                            cAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATc                                                <  1:206207/81‑1 (MQ=255)                             aGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATcc                                               >  2:206236/1‑81 (MQ=255)                             aGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATcc                                               >  1:206264/1‑81 (MQ=255)                             aGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATcc                                               <  2:206208/81‑1 (MQ=255)                                 tCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCAc                                           >  2:206230/1‑81 (MQ=255)                                         cGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATg                                   >  2:206246/1‑81 (MQ=255)                                          gTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGt                                  <  1:206209/81‑1 (MQ=255)                                             atatGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTc                                 >  1:206252/1‑79 (MQ=255)                                               atGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAg                              >  1:206244/1‑80 (MQ=255)                                                   gcTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGt                         >  2:206243/1‑81 (MQ=255)                                                                   ttACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGc          >  2:206239/1‑80 (MQ=255)                                                                   ttACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCg         <  2:370421/81‑1 (MQ=255)                                                                     aCTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGa        <  2:206211/80‑1 (MQ=255)                                                                     aCTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGa        <  2:206210/80‑1 (MQ=255)                                                                     aCTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGa        >  2:206248/1‑80 (MQ=255)                                                                          ggCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGAAAAAAg  <  1:206212/81‑1 (MQ=255)                                                                             |                                                                              TTGAGTGCAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCCGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGAAAAAAG  >  NC_000913/1096158‑1096311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑