Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,104,203	A→G	T85A (ACA→GCA)	csgB →	curlin, minor subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,104,203	0	A	G	100.0%	81.6 / NA	23	T85A (ACA→GCA)	csgB	curlin, minor subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/13);  total (10/13)

AGTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGG  >  NC_000913/1104126‑1104279                                                                              |                                                                             aGTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAg                                                                           >  2:207430/1‑81 (MQ=255)     aCGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAg                                                                           >  1:207429/1‑77 (MQ=255)       ggCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGAtt                                                                     <  1:207393/81‑1 (MQ=255)       ggCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGAt                                                                      >  1:207420/1‑80 (MQ=255)           gggAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATaa                                                                 <  1:207394/81‑1 (MQ=255)                  tCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCa                                                          >  1:207422/1‑81 (MQ=255)                    aaaaCTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCata                                                        >  1:207426/1‑81 (MQ=255)                    aaaaCTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCat                                                         <  1:207395/80‑1 (MQ=255)                     aaaCTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCatat                                                       <  2:207396/81‑1 (MQ=255)                     aaaCTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCata                                                        >  2:207424/1‑80 (MQ=255)                        cTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATAtt                                                    <  2:207397/81‑1 (MQ=255)                             ggCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGAt                                                 >  2:207428/1‑79 (MQ=255)                              gCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAg                                              <  2:207398/81‑1 (MQ=255)                                    gttgCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCggg                                         >  2:207433/1‑80 (MQ=255)                                          cAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGcc                                  <  2:207399/81‑1 (MQ=255)                                                ggTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGAt                            <  2:207400/81‑1 (MQ=255)                                                    gtagCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGcc                         <  1:207401/80‑1 (MQ=255)                                                          aCCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTAttt                  >  2:207435/1‑81 (MQ=255)                                                           ccGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTc                 <  1:207402/81‑1 (MQ=255)                                                                  aaGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGt          >  2:207436/1‑81 (MQ=255)                                                                    gATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGc        <  2:207403/81‑1 (MQ=255)                                                                         aCCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTAt    <  2:207404/80‑1 (MQ=255)                                                                          ccAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATgg  <  2:207405/81‑1 (MQ=255)                                                                              |                                                                             AGTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGG  >  NC_000913/1104126‑1104279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑